Identification of the biosynthetic gene clusters for the lipopeptides fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum
© 2014 The American Chemical Society and American Society of Pharmacognosy. The closely related species Fusarium graminearum and Fusarium pseudograminearum differ in that each contains a gene cluster with a polyketide synthase (PKS) and a nonribosomal peptide synthetase (NRPS) that is not present in...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
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American Chemical Society and American Society of Pharmacognosy
2014
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| Online Access: | http://hdl.handle.net/20.500.11937/67722 |
| _version_ | 1848761640953053184 |
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| author | Sørensen, J. Sondergaard, T. Covarelli, Lorenzo Fuertes, P. Hansen, F. Frandsen, R. Saei, W. Lukassen, M. Wimmer, R. Nielsen, K. Gardiner, D. Giese, H. |
| author_facet | Sørensen, J. Sondergaard, T. Covarelli, Lorenzo Fuertes, P. Hansen, F. Frandsen, R. Saei, W. Lukassen, M. Wimmer, R. Nielsen, K. Gardiner, D. Giese, H. |
| author_sort | Sørensen, J. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2014 The American Chemical Society and American Society of Pharmacognosy. The closely related species Fusarium graminearum and Fusarium pseudograminearum differ in that each contains a gene cluster with a polyketide synthase (PKS) and a nonribosomal peptide synthetase (NRPS) that is not present in the other species. To identify their products, we deleted PKS6 and NRPS7 in F. graminearum and NRPS32 in F. pseudograminearum. By comparing the secondary metabolite profiles of the strains we identified the resulting product in F. graminearum as fusaristatin A, and as W493 A and B in F. pseudograminearum. These lipopeptides have previously been isolated from unidentified Fusarium species. On the basis of genes in the putative gene clusters we propose a model for biosynthesis where the polyketide product is shuttled to the NPRS via a CoA ligase and a thioesterase in F. pseudograminearum. In F. graminearum the polyketide is proposed to be directly assimilated by the NRPS. |
| first_indexed | 2025-11-14T10:34:54Z |
| format | Journal Article |
| id | curtin-20.500.11937-67722 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:34:54Z |
| publishDate | 2014 |
| publisher | American Chemical Society and American Society of Pharmacognosy |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-677222018-05-18T08:06:23Z Identification of the biosynthetic gene clusters for the lipopeptides fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum Sørensen, J. Sondergaard, T. Covarelli, Lorenzo Fuertes, P. Hansen, F. Frandsen, R. Saei, W. Lukassen, M. Wimmer, R. Nielsen, K. Gardiner, D. Giese, H. © 2014 The American Chemical Society and American Society of Pharmacognosy. The closely related species Fusarium graminearum and Fusarium pseudograminearum differ in that each contains a gene cluster with a polyketide synthase (PKS) and a nonribosomal peptide synthetase (NRPS) that is not present in the other species. To identify their products, we deleted PKS6 and NRPS7 in F. graminearum and NRPS32 in F. pseudograminearum. By comparing the secondary metabolite profiles of the strains we identified the resulting product in F. graminearum as fusaristatin A, and as W493 A and B in F. pseudograminearum. These lipopeptides have previously been isolated from unidentified Fusarium species. On the basis of genes in the putative gene clusters we propose a model for biosynthesis where the polyketide product is shuttled to the NPRS via a CoA ligase and a thioesterase in F. pseudograminearum. In F. graminearum the polyketide is proposed to be directly assimilated by the NRPS. 2014 Journal Article http://hdl.handle.net/20.500.11937/67722 10.1021/np500436r American Chemical Society and American Society of Pharmacognosy restricted |
| spellingShingle | Sørensen, J. Sondergaard, T. Covarelli, Lorenzo Fuertes, P. Hansen, F. Frandsen, R. Saei, W. Lukassen, M. Wimmer, R. Nielsen, K. Gardiner, D. Giese, H. Identification of the biosynthetic gene clusters for the lipopeptides fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum |
| title | Identification of the biosynthetic gene clusters for the lipopeptides fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum |
| title_full | Identification of the biosynthetic gene clusters for the lipopeptides fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum |
| title_fullStr | Identification of the biosynthetic gene clusters for the lipopeptides fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum |
| title_full_unstemmed | Identification of the biosynthetic gene clusters for the lipopeptides fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum |
| title_short | Identification of the biosynthetic gene clusters for the lipopeptides fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum |
| title_sort | identification of the biosynthetic gene clusters for the lipopeptides fusaristatin a and w493 b in fusarium graminearum and f. pseudograminearum |
| url | http://hdl.handle.net/20.500.11937/67722 |