Development of genetic SSR markers in Blumeria graminis f. sp. hordei and application to isolates from Australia
The barley powdery mildew pathogen, Blumeria graminis f. sp. hordei (Bgh), exists in numerous haplotypes and displays significant differences in fungicide sensitivity. It causes considerable yield losses throughout the world. Microsatellite SSRs are useful tools to study the population level and bio...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
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Wiley-Blackwell
2015
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| Online Access: | http://hdl.handle.net/20.500.11937/6764 |
| _version_ | 1848745171427000320 |
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| author | Tucker, Madeline Moffat, Caroline Ellwood, Simon Tan, Kar-Chun Jayasena, K. Oliver, Richard |
| author_facet | Tucker, Madeline Moffat, Caroline Ellwood, Simon Tan, Kar-Chun Jayasena, K. Oliver, Richard |
| author_sort | Tucker, Madeline |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The barley powdery mildew pathogen, Blumeria graminis f. sp. hordei (Bgh), exists in numerous haplotypes and displays significant differences in fungicide sensitivity. It causes considerable yield losses throughout the world. Microsatellite SSRs are useful tools to study the population level and biogeographic aspects of intraspecific diversity, but so far none have been defined for Bgh. Here, eight polymorphic microsatellite loci were identified and characterized. Primer pairs amplifying the loci were then applied to 111 isolates of Bgh from Australia. The number of alleles per locus ranged from 4 to 13, and Nei's genetic diversity ranged from 0·25 to 0·76. The microsatellite primers detected several clones among the isolates and defined 97 unique haplotypes. There was little evidence for regional genotypic subdivision, suggesting that gene flow may not be restricted among geographic regions. All data was consistent with high levels of genetic diversity, potentially resulting from random mating and spread within each region. |
| first_indexed | 2025-11-14T06:13:07Z |
| format | Journal Article |
| id | curtin-20.500.11937-6764 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:13:07Z |
| publishDate | 2015 |
| publisher | Wiley-Blackwell |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-67642017-09-13T14:36:24Z Development of genetic SSR markers in Blumeria graminis f. sp. hordei and application to isolates from Australia Tucker, Madeline Moffat, Caroline Ellwood, Simon Tan, Kar-Chun Jayasena, K. Oliver, Richard microsatellite Barley powdery mildew population The barley powdery mildew pathogen, Blumeria graminis f. sp. hordei (Bgh), exists in numerous haplotypes and displays significant differences in fungicide sensitivity. It causes considerable yield losses throughout the world. Microsatellite SSRs are useful tools to study the population level and biogeographic aspects of intraspecific diversity, but so far none have been defined for Bgh. Here, eight polymorphic microsatellite loci were identified and characterized. Primer pairs amplifying the loci were then applied to 111 isolates of Bgh from Australia. The number of alleles per locus ranged from 4 to 13, and Nei's genetic diversity ranged from 0·25 to 0·76. The microsatellite primers detected several clones among the isolates and defined 97 unique haplotypes. There was little evidence for regional genotypic subdivision, suggesting that gene flow may not be restricted among geographic regions. All data was consistent with high levels of genetic diversity, potentially resulting from random mating and spread within each region. 2015 Journal Article http://hdl.handle.net/20.500.11937/6764 10.1111/ppa.12258 Wiley-Blackwell restricted |
| spellingShingle | microsatellite Barley powdery mildew population Tucker, Madeline Moffat, Caroline Ellwood, Simon Tan, Kar-Chun Jayasena, K. Oliver, Richard Development of genetic SSR markers in Blumeria graminis f. sp. hordei and application to isolates from Australia |
| title | Development of genetic SSR markers in Blumeria graminis f. sp. hordei and application to isolates from Australia |
| title_full | Development of genetic SSR markers in Blumeria graminis f. sp. hordei and application to isolates from Australia |
| title_fullStr | Development of genetic SSR markers in Blumeria graminis f. sp. hordei and application to isolates from Australia |
| title_full_unstemmed | Development of genetic SSR markers in Blumeria graminis f. sp. hordei and application to isolates from Australia |
| title_short | Development of genetic SSR markers in Blumeria graminis f. sp. hordei and application to isolates from Australia |
| title_sort | development of genetic ssr markers in blumeria graminis f. sp. hordei and application to isolates from australia |
| topic | microsatellite Barley powdery mildew population |
| url | http://hdl.handle.net/20.500.11937/6764 |