Polyclonal emergence of vanA vancomycin-resistant Enterococcus faecium in Australia

Objectives: To investigate the genetic context associated with the emergence of vanA VRE in Australia. Methods: The whole genomes of 18 randomly selected vanA-positive Enterococcus faecium patient isolates, collected between 2011 and 2013 from hospitals in four Australian capitals, were sequenced an...

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Main Authors: van Hal, S., Espedido, B., Coombs, Geoffrey, Howden, B., Korman, T., Nimmo, G., Gosbell, I., Jensen, S.
Format: Journal Article
Published: Oxford University Press 2017
Online Access:http://hdl.handle.net/20.500.11937/67550
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author van Hal, S.
Espedido, B.
Coombs, Geoffrey
Howden, B.
Korman, T.
Nimmo, G.
Gosbell, I.
Jensen, S.
author_facet van Hal, S.
Espedido, B.
Coombs, Geoffrey
Howden, B.
Korman, T.
Nimmo, G.
Gosbell, I.
Jensen, S.
author_sort van Hal, S.
building Curtin Institutional Repository
collection Online Access
description Objectives: To investigate the genetic context associated with the emergence of vanA VRE in Australia. Methods: The whole genomes of 18 randomly selected vanA-positive Enterococcus faecium patient isolates, collected between 2011 and 2013 from hospitals in four Australian capitals, were sequenced and analysed. Results: In silico typing and transposon/plasmid assembly revealed that the sequenced isolates represented (in most cases) different hospital-adapted STs and were associated with a variety of different Tn1546 variants and plasmid backbone structures. Conclusions: The recent emergence of vanA VRE in Australia was polyclonal and not associatedwith the dissemination of a single 'dominant' ST or vanA-encoding plasmid. Interestingly, the factors contributing to this epidemiological change are not known and future studies may need to consider investigation of potential community sources.
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institution Curtin University Malaysia
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publishDate 2017
publisher Oxford University Press
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spelling curtin-20.500.11937-675502018-09-26T01:01:33Z Polyclonal emergence of vanA vancomycin-resistant Enterococcus faecium in Australia van Hal, S. Espedido, B. Coombs, Geoffrey Howden, B. Korman, T. Nimmo, G. Gosbell, I. Jensen, S. Objectives: To investigate the genetic context associated with the emergence of vanA VRE in Australia. Methods: The whole genomes of 18 randomly selected vanA-positive Enterococcus faecium patient isolates, collected between 2011 and 2013 from hospitals in four Australian capitals, were sequenced and analysed. Results: In silico typing and transposon/plasmid assembly revealed that the sequenced isolates represented (in most cases) different hospital-adapted STs and were associated with a variety of different Tn1546 variants and plasmid backbone structures. Conclusions: The recent emergence of vanA VRE in Australia was polyclonal and not associatedwith the dissemination of a single 'dominant' ST or vanA-encoding plasmid. Interestingly, the factors contributing to this epidemiological change are not known and future studies may need to consider investigation of potential community sources. 2017 Journal Article http://hdl.handle.net/20.500.11937/67550 10.1093/jac/dkw539 Oxford University Press restricted
spellingShingle van Hal, S.
Espedido, B.
Coombs, Geoffrey
Howden, B.
Korman, T.
Nimmo, G.
Gosbell, I.
Jensen, S.
Polyclonal emergence of vanA vancomycin-resistant Enterococcus faecium in Australia
title Polyclonal emergence of vanA vancomycin-resistant Enterococcus faecium in Australia
title_full Polyclonal emergence of vanA vancomycin-resistant Enterococcus faecium in Australia
title_fullStr Polyclonal emergence of vanA vancomycin-resistant Enterococcus faecium in Australia
title_full_unstemmed Polyclonal emergence of vanA vancomycin-resistant Enterococcus faecium in Australia
title_short Polyclonal emergence of vanA vancomycin-resistant Enterococcus faecium in Australia
title_sort polyclonal emergence of vana vancomycin-resistant enterococcus faecium in australia
url http://hdl.handle.net/20.500.11937/67550