Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture
Sucrose has several advantages over glucose as a feedstock for bioprocesses, both environmentally and economically. However, most industrial Escherichia coli strains are unable to utilize sucrose. E. coli W can grow on sucrose but stops growing when sucrose concentrations become low. This is undesir...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
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Elsevier Science
2010
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| Online Access: | http://hdl.handle.net/20.500.11937/6726 |
| _version_ | 1848745160304754688 |
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| author | Arifin, Yalun Sabri, S. Sugiarto, H. Krömer, J. Vickers, C. Nielsen, L. |
| author_facet | Arifin, Yalun Sabri, S. Sugiarto, H. Krömer, J. Vickers, C. Nielsen, L. |
| author_sort | Arifin, Yalun |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Sucrose has several advantages over glucose as a feedstock for bioprocesses, both environmentally and economically. However, most industrial Escherichia coli strains are unable to utilize sucrose. E. coli W can grow on sucrose but stops growing when sucrose concentrations become low. This is undesirable in fed-batch conditions where sugar levels are low between feeding pulses. Sucrose uptake rates were improved by removal of the cscR gene, which encodes a protein that represses expression of the sucrose utilization genes at low sucrose concentrations. Poly-3-hydroxybutyrate (PHB) was used as a model compound in order to assess the effect of improved sugar utilization on bio-production. In the cscR knockout strain, production from sucrose was improved by 50%; this strain also produced 30% more PHB than the wild-type using glucose. This result demonstrates the feasibility of utilizing sucrose as an industrial feedstock for E. coli-based bioprocesses in high cell density culture. © 2011. |
| first_indexed | 2025-11-14T06:12:56Z |
| format | Journal Article |
| id | curtin-20.500.11937-6726 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:12:56Z |
| publishDate | 2010 |
| publisher | Elsevier Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-67262017-09-13T14:35:42Z Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture Arifin, Yalun Sabri, S. Sugiarto, H. Krömer, J. Vickers, C. Nielsen, L. Sucrose has several advantages over glucose as a feedstock for bioprocesses, both environmentally and economically. However, most industrial Escherichia coli strains are unable to utilize sucrose. E. coli W can grow on sucrose but stops growing when sucrose concentrations become low. This is undesirable in fed-batch conditions where sugar levels are low between feeding pulses. Sucrose uptake rates were improved by removal of the cscR gene, which encodes a protein that represses expression of the sucrose utilization genes at low sucrose concentrations. Poly-3-hydroxybutyrate (PHB) was used as a model compound in order to assess the effect of improved sugar utilization on bio-production. In the cscR knockout strain, production from sucrose was improved by 50%; this strain also produced 30% more PHB than the wild-type using glucose. This result demonstrates the feasibility of utilizing sucrose as an industrial feedstock for E. coli-based bioprocesses in high cell density culture. © 2011. 2010 Journal Article http://hdl.handle.net/20.500.11937/6726 10.1016/j.jbiotec.2011.07.003 Elsevier Science restricted |
| spellingShingle | Arifin, Yalun Sabri, S. Sugiarto, H. Krömer, J. Vickers, C. Nielsen, L. Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture |
| title | Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture |
| title_full | Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture |
| title_fullStr | Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture |
| title_full_unstemmed | Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture |
| title_short | Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture |
| title_sort | deletion of cscr in escherichia coli w improves growth and poly-3-hydroxybutyrate (phb) production from sucrose in fed batch culture |
| url | http://hdl.handle.net/20.500.11937/6726 |