Isolation and characterization of urease-producing bacteria from tropical peat
Urease were known to catalyze the conversion of urea to ammonia and carbon dioxide. Microbial urease has demonstrated its benefits in wide biotechnological, agricultural, medicinal and engineering application. There are number of diverse microbial species contribute to urease activity in different n...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Published: |
2018
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| Online Access: | http://hdl.handle.net/20.500.11937/67201 |
| _version_ | 1848761502737104896 |
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| author | Phang, I. Chan, Stephanie Wong, K. Lau, S. |
| author_facet | Phang, I. Chan, Stephanie Wong, K. Lau, S. |
| author_sort | Phang, I. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Urease were known to catalyze the conversion of urea to ammonia and carbon dioxide. Microbial urease has demonstrated its benefits in wide biotechnological, agricultural, medicinal and engineering application. There are number of diverse microbial species contribute to urease activity in different natural habitats like soil, ocean and in various geological formation. For this study, urease bacteria were screened and isolate from acidic peat in Sarawak, Malaysia. Five distinct and diverse bacterial strains that were able to produce urease constitutively were selected to be characterized with respect to morphology, biochemical test, growth conditions and urease activity. The selected strain showed their capability to precipitate calcium carbonate (CaCO 3 ). Hence, the isolates could be potential source of acid ureases that can be use in various industrial utilizations. 16S rRNA sequencing and phylogenetic analysis found that the selected isolates belong to the genus of Bacillus. |
| first_indexed | 2025-11-14T10:32:42Z |
| format | Journal Article |
| id | curtin-20.500.11937-67201 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:32:42Z |
| publishDate | 2018 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-672012018-09-28T02:45:59Z Isolation and characterization of urease-producing bacteria from tropical peat Phang, I. Chan, Stephanie Wong, K. Lau, S. Urease were known to catalyze the conversion of urea to ammonia and carbon dioxide. Microbial urease has demonstrated its benefits in wide biotechnological, agricultural, medicinal and engineering application. There are number of diverse microbial species contribute to urease activity in different natural habitats like soil, ocean and in various geological formation. For this study, urease bacteria were screened and isolate from acidic peat in Sarawak, Malaysia. Five distinct and diverse bacterial strains that were able to produce urease constitutively were selected to be characterized with respect to morphology, biochemical test, growth conditions and urease activity. The selected strain showed their capability to precipitate calcium carbonate (CaCO 3 ). Hence, the isolates could be potential source of acid ureases that can be use in various industrial utilizations. 16S rRNA sequencing and phylogenetic analysis found that the selected isolates belong to the genus of Bacillus. 2018 Journal Article http://hdl.handle.net/20.500.11937/67201 10.1016/j.bcab.2017.12.006 restricted |
| spellingShingle | Phang, I. Chan, Stephanie Wong, K. Lau, S. Isolation and characterization of urease-producing bacteria from tropical peat |
| title | Isolation and characterization of urease-producing bacteria from tropical peat |
| title_full | Isolation and characterization of urease-producing bacteria from tropical peat |
| title_fullStr | Isolation and characterization of urease-producing bacteria from tropical peat |
| title_full_unstemmed | Isolation and characterization of urease-producing bacteria from tropical peat |
| title_short | Isolation and characterization of urease-producing bacteria from tropical peat |
| title_sort | isolation and characterization of urease-producing bacteria from tropical peat |
| url | http://hdl.handle.net/20.500.11937/67201 |