Chromatographic characterisation of aptamer-modified poly(EDMA-co-GMA) monolithic disk format for protein binding and separation
The introduction of aptameric ligands onto disk-monolithic adsorbent, representing a unique strategy for convective isolation of target molecules with high specificity and selectivity, is investigated for the first time. Experimental results showed that the disk monolith possessed a good permeabilit...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Published: |
Taylor & Francis Inc.
2018
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| Online Access: | http://hdl.handle.net/20.500.11937/66847 |
| _version_ | 1848761408126189568 |
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| author | Acquah, C. Danquah, M. Chan, Y. Loo Chin Moy, Charles Ongkudon, C. Lau, John |
| author_facet | Acquah, C. Danquah, M. Chan, Y. Loo Chin Moy, Charles Ongkudon, C. Lau, John |
| author_sort | Acquah, C. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The introduction of aptameric ligands onto disk-monolithic adsorbent, representing a unique strategy for convective isolation of target molecules with high specificity and selectivity, is investigated for the first time. Experimental results showed that the disk monolith possessed a good permeability of 1.67 ± 0.05 × 10 –14 m 2 (RSD = 3.2%). The aptameric ligand density for the aptamer-modified disk monolith was 480 pmol/uL. Chromatographic analysis of the aptamer disk-monolith efficiency showed an optimum linear velocity of 126 cm/min (˜0.25 mL/min) at room temperatures 25 ± 2°C. The theoretical number of plates corresponding to the optimum linear velocity was 128.2 with an height equivalent to the theoretical plate of 0.022 mm. The disk aptamer-immobilised monolithic system demonstrated good selectivity and isolation of thrombin from non-targets. |
| first_indexed | 2025-11-14T10:31:12Z |
| format | Journal Article |
| id | curtin-20.500.11937-66847 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:31:12Z |
| publishDate | 2018 |
| publisher | Taylor & Francis Inc. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-668472019-01-07T06:29:21Z Chromatographic characterisation of aptamer-modified poly(EDMA-co-GMA) monolithic disk format for protein binding and separation Acquah, C. Danquah, M. Chan, Y. Loo Chin Moy, Charles Ongkudon, C. Lau, John The introduction of aptameric ligands onto disk-monolithic adsorbent, representing a unique strategy for convective isolation of target molecules with high specificity and selectivity, is investigated for the first time. Experimental results showed that the disk monolith possessed a good permeability of 1.67 ± 0.05 × 10 –14 m 2 (RSD = 3.2%). The aptameric ligand density for the aptamer-modified disk monolith was 480 pmol/uL. Chromatographic analysis of the aptamer disk-monolith efficiency showed an optimum linear velocity of 126 cm/min (˜0.25 mL/min) at room temperatures 25 ± 2°C. The theoretical number of plates corresponding to the optimum linear velocity was 128.2 with an height equivalent to the theoretical plate of 0.022 mm. The disk aptamer-immobilised monolithic system demonstrated good selectivity and isolation of thrombin from non-targets. 2018 Journal Article http://hdl.handle.net/20.500.11937/66847 10.1080/01496395.2018.1443139 Taylor & Francis Inc. restricted |
| spellingShingle | Acquah, C. Danquah, M. Chan, Y. Loo Chin Moy, Charles Ongkudon, C. Lau, John Chromatographic characterisation of aptamer-modified poly(EDMA-co-GMA) monolithic disk format for protein binding and separation |
| title | Chromatographic characterisation of aptamer-modified poly(EDMA-co-GMA) monolithic disk format for protein binding and separation |
| title_full | Chromatographic characterisation of aptamer-modified poly(EDMA-co-GMA) monolithic disk format for protein binding and separation |
| title_fullStr | Chromatographic characterisation of aptamer-modified poly(EDMA-co-GMA) monolithic disk format for protein binding and separation |
| title_full_unstemmed | Chromatographic characterisation of aptamer-modified poly(EDMA-co-GMA) monolithic disk format for protein binding and separation |
| title_short | Chromatographic characterisation of aptamer-modified poly(EDMA-co-GMA) monolithic disk format for protein binding and separation |
| title_sort | chromatographic characterisation of aptamer-modified poly(edma-co-gma) monolithic disk format for protein binding and separation |
| url | http://hdl.handle.net/20.500.11937/66847 |