Characterization of mineral phosphate solubilization traits from a barley rhizosphere soil functional metagenome
Mineral phosphate solubilization (MPS) microorganisms are important for their provision of orthophosphate anions for plant growth promotion activity in soil. In this study, we applied a functional metagenomic approach to identify this trait directly from the microbiome in barley rhizosphere soil tha...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
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John Wiley & Sons LTD
2013
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/6682 |
| _version_ | 1848745147887517696 |
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| author | Chhabra, Sagar Brazil, Dina Morrissey, John Burke, James O'Gara, Fergal Dowling, David |
| author_facet | Chhabra, Sagar Brazil, Dina Morrissey, John Burke, James O'Gara, Fergal Dowling, David |
| author_sort | Chhabra, Sagar |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Mineral phosphate solubilization (MPS) microorganisms are important for their provision of orthophosphate anions for plant growth promotion activity in soil. In this study, we applied a functional metagenomic approach to identify this trait directly from the microbiome in barley rhizosphere soil that had not received P fertilizer over a 15-year period. A fosmid system was used to clone the metagenome of which 18,000 clones (~666 Mb of DNA) was screened for MPS. Functional assays and High Performance Liquid Chromatography analysis recognized gluconic acid production and MPS activity in the range 24.8–77.1 mmol/L and 27.6–38.16 µg/mL, respectively, when screened in an Escherichia coli host (at frequency of one MPS-positive clone hit per 114 Mb DNA tested). The MPS clones (with average insert size of ~37 kb) were analysed by 454 Roche sequencing and annotated. A number of genes/operons with homology to Phosphorous (P) uptake, regulatory and solubilization mechanisms were identified, linking the MPS function to the uncultivated microbiome present in barley rhizosphere soil. |
| first_indexed | 2025-11-14T06:12:45Z |
| format | Journal Article |
| id | curtin-20.500.11937-6682 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:12:45Z |
| publishDate | 2013 |
| publisher | John Wiley & Sons LTD |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-66822017-09-13T14:36:24Z Characterization of mineral phosphate solubilization traits from a barley rhizosphere soil functional metagenome Chhabra, Sagar Brazil, Dina Morrissey, John Burke, James O'Gara, Fergal Dowling, David MPS pyrroloquinoline quinone Glucose dehydrogenase soil metagenome Mineral phosphate solubilization (MPS) microorganisms are important for their provision of orthophosphate anions for plant growth promotion activity in soil. In this study, we applied a functional metagenomic approach to identify this trait directly from the microbiome in barley rhizosphere soil that had not received P fertilizer over a 15-year period. A fosmid system was used to clone the metagenome of which 18,000 clones (~666 Mb of DNA) was screened for MPS. Functional assays and High Performance Liquid Chromatography analysis recognized gluconic acid production and MPS activity in the range 24.8–77.1 mmol/L and 27.6–38.16 µg/mL, respectively, when screened in an Escherichia coli host (at frequency of one MPS-positive clone hit per 114 Mb DNA tested). The MPS clones (with average insert size of ~37 kb) were analysed by 454 Roche sequencing and annotated. A number of genes/operons with homology to Phosphorous (P) uptake, regulatory and solubilization mechanisms were identified, linking the MPS function to the uncultivated microbiome present in barley rhizosphere soil. 2013 Journal Article http://hdl.handle.net/20.500.11937/6682 10.1002/mbo3.110 John Wiley & Sons LTD unknown |
| spellingShingle | MPS pyrroloquinoline quinone Glucose dehydrogenase soil metagenome Chhabra, Sagar Brazil, Dina Morrissey, John Burke, James O'Gara, Fergal Dowling, David Characterization of mineral phosphate solubilization traits from a barley rhizosphere soil functional metagenome |
| title | Characterization of mineral phosphate solubilization traits from a barley rhizosphere soil functional metagenome |
| title_full | Characterization of mineral phosphate solubilization traits from a barley rhizosphere soil functional metagenome |
| title_fullStr | Characterization of mineral phosphate solubilization traits from a barley rhizosphere soil functional metagenome |
| title_full_unstemmed | Characterization of mineral phosphate solubilization traits from a barley rhizosphere soil functional metagenome |
| title_short | Characterization of mineral phosphate solubilization traits from a barley rhizosphere soil functional metagenome |
| title_sort | characterization of mineral phosphate solubilization traits from a barley rhizosphere soil functional metagenome |
| topic | MPS pyrroloquinoline quinone Glucose dehydrogenase soil metagenome |
| url | http://hdl.handle.net/20.500.11937/6682 |