Measuring metabolic rates of small terrestrial organisms by fluorescence-based closed-system respirometry.
We explore a recent innovative variation of closed-system respirometry for terrestrial organisms, whereby pO2is repeatedly measured fluorometrically in a constant-volume chamber over multiple time points. We outline a protocol that aligns this technology with the broader literature on aerial respiro...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Published: |
The Company of Biologists Ltd.
2018
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| Online Access: | http://purl.org/au-research/grants/arc/IC150100041 http://hdl.handle.net/20.500.11937/66518 |
| _version_ | 1848761341028859904 |
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| author | Tomlinson, Sean Dalziell, Emma Withers, P. Lewandrowski, W. Dixon, Kingsley Merritt, D. |
| author_facet | Tomlinson, Sean Dalziell, Emma Withers, P. Lewandrowski, W. Dixon, Kingsley Merritt, D. |
| author_sort | Tomlinson, Sean |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | We explore a recent innovative variation of closed-system respirometry for terrestrial organisms, whereby pO2is repeatedly measured fluorometrically in a constant-volume chamber over multiple time points. We outline a protocol that aligns this technology with the broader literature on aerial respirometry, including the calculations required to accurately convert O2depletion to metabolic rate (MR). We identify a series of assumptions, and sources of error associated with this technique, including thresholds where O2depletion becomes limiting, that impart errors to the calculation and interpretation of MR. Using these adjusted calculations, we found that the resting MR of five species of angiosperm seeds ranged from 0.011 to 0.640 mL.g-1h-1, consistent with published seed MR. This innovative methodology greatly expands the lower size limit of terrestrial organisms that can be measured, and offers the potential for measuring MR changes over time as a result of physiological processes of the organism. |
| first_indexed | 2025-11-14T10:30:08Z |
| format | Journal Article |
| id | curtin-20.500.11937-66518 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:30:08Z |
| publishDate | 2018 |
| publisher | The Company of Biologists Ltd. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-665182023-04-26T07:08:23Z Measuring metabolic rates of small terrestrial organisms by fluorescence-based closed-system respirometry. Tomlinson, Sean Dalziell, Emma Withers, P. Lewandrowski, W. Dixon, Kingsley Merritt, D. We explore a recent innovative variation of closed-system respirometry for terrestrial organisms, whereby pO2is repeatedly measured fluorometrically in a constant-volume chamber over multiple time points. We outline a protocol that aligns this technology with the broader literature on aerial respirometry, including the calculations required to accurately convert O2depletion to metabolic rate (MR). We identify a series of assumptions, and sources of error associated with this technique, including thresholds where O2depletion becomes limiting, that impart errors to the calculation and interpretation of MR. Using these adjusted calculations, we found that the resting MR of five species of angiosperm seeds ranged from 0.011 to 0.640 mL.g-1h-1, consistent with published seed MR. This innovative methodology greatly expands the lower size limit of terrestrial organisms that can be measured, and offers the potential for measuring MR changes over time as a result of physiological processes of the organism. 2018 Journal Article http://hdl.handle.net/20.500.11937/66518 10.1242/jeb.172874 http://purl.org/au-research/grants/arc/IC150100041 http://purl.org/au-research/grants/arc/LP160100381 The Company of Biologists Ltd. fulltext |
| spellingShingle | Tomlinson, Sean Dalziell, Emma Withers, P. Lewandrowski, W. Dixon, Kingsley Merritt, D. Measuring metabolic rates of small terrestrial organisms by fluorescence-based closed-system respirometry. |
| title | Measuring metabolic rates of small terrestrial organisms by fluorescence-based closed-system respirometry. |
| title_full | Measuring metabolic rates of small terrestrial organisms by fluorescence-based closed-system respirometry. |
| title_fullStr | Measuring metabolic rates of small terrestrial organisms by fluorescence-based closed-system respirometry. |
| title_full_unstemmed | Measuring metabolic rates of small terrestrial organisms by fluorescence-based closed-system respirometry. |
| title_short | Measuring metabolic rates of small terrestrial organisms by fluorescence-based closed-system respirometry. |
| title_sort | measuring metabolic rates of small terrestrial organisms by fluorescence-based closed-system respirometry. |
| url | http://purl.org/au-research/grants/arc/IC150100041 http://purl.org/au-research/grants/arc/IC150100041 http://hdl.handle.net/20.500.11937/66518 |