Improved biosynthesis of silver nanoparticles using keratinase from Stenotrophomonas maltophilia R13: reaction optimization, structural characterization, and biomedical activity
© 2017 Springer-Verlag GmbH Germany, part of Springer Nature In the present study, keratinase from Stenotrophomonas maltophilia R13 was used for the first time as a reducing agent for the eco-friendly synthesis of AgNPs. The keratinase produced by strain R13 was responsible for the reduction of silv...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11937/66369 |
| _version_ | 1848761305827115008 |
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| author | Jang, E. Son, Y. Park, S. Yoo, J. Cho, Y. Jeong, S. Liu, Shaomin Son, H. |
| author_facet | Jang, E. Son, Y. Park, S. Yoo, J. Cho, Y. Jeong, S. Liu, Shaomin Son, H. |
| author_sort | Jang, E. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2017 Springer-Verlag GmbH Germany, part of Springer Nature In the present study, keratinase from Stenotrophomonas maltophilia R13 was used for the first time as a reducing agent for the eco-friendly synthesis of AgNPs. The keratinase produced by strain R13 was responsible for the reduction of silver ions and the subsequent formation of AgNPs. Maximum AgNP synthesis was achieved using 2 mM AgNO 3 at pH 9 and 40 °C. Electron microscopy and dynamic light scattering analysis showed AgNPs were spherical and of average diameter ~ 8.4 nm. X-ray diffraction revealed that AgNPs were crystalline. FTIR indicated AgNPs were stabilized by proteins present in the crude enzyme solution of strain R13. AgNPs exhibited a broad antimicrobial spectrum against several pathogenic microorganisms, and the antimicrobial mechanism appeared to involve structural deformation of cells resulting in membrane leakage and subsequent lysis. AgNPs also displayed 1,1-diphenyl-2-picrylhydrazyl (IC 50 = 0.0112 mg/ml), 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonate radical scavenging (IC 50 = 0.0243 mg/ml), and anti-collagenase (IC 50 = 23.5 mg/ml) activities. |
| first_indexed | 2025-11-14T10:29:34Z |
| format | Journal Article |
| id | curtin-20.500.11937-66369 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:29:34Z |
| publishDate | 2017 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-663692018-04-30T02:48:45Z Improved biosynthesis of silver nanoparticles using keratinase from Stenotrophomonas maltophilia R13: reaction optimization, structural characterization, and biomedical activity Jang, E. Son, Y. Park, S. Yoo, J. Cho, Y. Jeong, S. Liu, Shaomin Son, H. © 2017 Springer-Verlag GmbH Germany, part of Springer Nature In the present study, keratinase from Stenotrophomonas maltophilia R13 was used for the first time as a reducing agent for the eco-friendly synthesis of AgNPs. The keratinase produced by strain R13 was responsible for the reduction of silver ions and the subsequent formation of AgNPs. Maximum AgNP synthesis was achieved using 2 mM AgNO 3 at pH 9 and 40 °C. Electron microscopy and dynamic light scattering analysis showed AgNPs were spherical and of average diameter ~ 8.4 nm. X-ray diffraction revealed that AgNPs were crystalline. FTIR indicated AgNPs were stabilized by proteins present in the crude enzyme solution of strain R13. AgNPs exhibited a broad antimicrobial spectrum against several pathogenic microorganisms, and the antimicrobial mechanism appeared to involve structural deformation of cells resulting in membrane leakage and subsequent lysis. AgNPs also displayed 1,1-diphenyl-2-picrylhydrazyl (IC 50 = 0.0112 mg/ml), 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonate radical scavenging (IC 50 = 0.0243 mg/ml), and anti-collagenase (IC 50 = 23.5 mg/ml) activities. 2017 Journal Article http://hdl.handle.net/20.500.11937/66369 10.1007/s00449-017-1873-0 restricted |
| spellingShingle | Jang, E. Son, Y. Park, S. Yoo, J. Cho, Y. Jeong, S. Liu, Shaomin Son, H. Improved biosynthesis of silver nanoparticles using keratinase from Stenotrophomonas maltophilia R13: reaction optimization, structural characterization, and biomedical activity |
| title | Improved biosynthesis of silver nanoparticles using keratinase from Stenotrophomonas maltophilia R13: reaction optimization, structural characterization, and biomedical activity |
| title_full | Improved biosynthesis of silver nanoparticles using keratinase from Stenotrophomonas maltophilia R13: reaction optimization, structural characterization, and biomedical activity |
| title_fullStr | Improved biosynthesis of silver nanoparticles using keratinase from Stenotrophomonas maltophilia R13: reaction optimization, structural characterization, and biomedical activity |
| title_full_unstemmed | Improved biosynthesis of silver nanoparticles using keratinase from Stenotrophomonas maltophilia R13: reaction optimization, structural characterization, and biomedical activity |
| title_short | Improved biosynthesis of silver nanoparticles using keratinase from Stenotrophomonas maltophilia R13: reaction optimization, structural characterization, and biomedical activity |
| title_sort | improved biosynthesis of silver nanoparticles using keratinase from stenotrophomonas maltophilia r13: reaction optimization, structural characterization, and biomedical activity |
| url | http://hdl.handle.net/20.500.11937/66369 |