Novel and divergent viruses associated with Australian orchid-fungus symbioses
© 2017 Elsevier B.V. Terrestrial orchids represent a symbiotic union between plants and mycorrhizal fungi. This study describes the occurrence and nature of viruses associated with one population of wild Pterostylis sanguinea orchids, including their fungal symbionts, over two consecutive years. A g...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
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Elsevier Science BV
2018
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| Online Access: | http://hdl.handle.net/20.500.11937/66281 |
| _version_ | 1848761283493494784 |
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| author | Ong, J. Li, H. Sivasithamparam, K. Dixon, Kingsley Jones, M. Wylie, S. |
| author_facet | Ong, J. Li, H. Sivasithamparam, K. Dixon, Kingsley Jones, M. Wylie, S. |
| author_sort | Ong, J. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2017 Elsevier B.V. Terrestrial orchids represent a symbiotic union between plants and mycorrhizal fungi. This study describes the occurrence and nature of viruses associated with one population of wild Pterostylis sanguinea orchids, including their fungal symbionts, over two consecutive years. A generic sequencing approach, which combined dsRNA-enrichment from plant and mycelial tissues, random amplification and high throughput shotgun sequencing was used to identify novel viruses. The majority of the virus-like sequences represent partial genomes, and their identification is based solely on de novo assembly of sequencing data. In orchid leaf tissues we found three isolates of a novel totivirus and an unclassified virus; both resemble fungus-infecting viruses. Two isolates of Ceratobasidium sp that were isolated from orchid underground stems contained at least 20 viruses, 16 of which were previously described as alphapartitiviruses and betapartitiviruses. A novel hypovirus and a mitovirus were genetically distant from existing members of the genera and did not readily fit into recognised subgroups. |
| first_indexed | 2025-11-14T10:29:13Z |
| format | Journal Article |
| id | curtin-20.500.11937-66281 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:29:13Z |
| publishDate | 2018 |
| publisher | Elsevier Science BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-662812018-04-30T02:48:46Z Novel and divergent viruses associated with Australian orchid-fungus symbioses Ong, J. Li, H. Sivasithamparam, K. Dixon, Kingsley Jones, M. Wylie, S. © 2017 Elsevier B.V. Terrestrial orchids represent a symbiotic union between plants and mycorrhizal fungi. This study describes the occurrence and nature of viruses associated with one population of wild Pterostylis sanguinea orchids, including their fungal symbionts, over two consecutive years. A generic sequencing approach, which combined dsRNA-enrichment from plant and mycelial tissues, random amplification and high throughput shotgun sequencing was used to identify novel viruses. The majority of the virus-like sequences represent partial genomes, and their identification is based solely on de novo assembly of sequencing data. In orchid leaf tissues we found three isolates of a novel totivirus and an unclassified virus; both resemble fungus-infecting viruses. Two isolates of Ceratobasidium sp that were isolated from orchid underground stems contained at least 20 viruses, 16 of which were previously described as alphapartitiviruses and betapartitiviruses. A novel hypovirus and a mitovirus were genetically distant from existing members of the genera and did not readily fit into recognised subgroups. 2018 Journal Article http://hdl.handle.net/20.500.11937/66281 10.1016/j.virusres.2017.11.026 Elsevier Science BV restricted |
| spellingShingle | Ong, J. Li, H. Sivasithamparam, K. Dixon, Kingsley Jones, M. Wylie, S. Novel and divergent viruses associated with Australian orchid-fungus symbioses |
| title | Novel and divergent viruses associated with Australian orchid-fungus symbioses |
| title_full | Novel and divergent viruses associated with Australian orchid-fungus symbioses |
| title_fullStr | Novel and divergent viruses associated with Australian orchid-fungus symbioses |
| title_full_unstemmed | Novel and divergent viruses associated with Australian orchid-fungus symbioses |
| title_short | Novel and divergent viruses associated with Australian orchid-fungus symbioses |
| title_sort | novel and divergent viruses associated with australian orchid-fungus symbioses |
| url | http://hdl.handle.net/20.500.11937/66281 |