Identification of QTLs associated with resistance to Phomopsis pod blight (Diaporthe toxica) in Lupinus albus
Phomopsis blight in Lupinus albus is caused by a fungal pathogen, Diaporthe toxica. It can invade all plant parts, leading to plant material becoming toxic to grazing animals, and potentially resulting in lupinosis. Identifying sources of resistance and breeding for resistance remains the best strat...
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| Format: | Journal Article |
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Nihon Ikushu Gakkai
2014
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| Online Access: | https://www.jstage.jst.go.jp/article/jsbbs/64/1/64_83/_article http://hdl.handle.net/20.500.11937/6365 |
| _version_ | 1848745055436668928 |
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| author | Cowley, R. Luckett, D. Ash, G. Harper, J. Vipin, C. Raman, H. Ellwood, Simon |
| author_facet | Cowley, R. Luckett, D. Ash, G. Harper, J. Vipin, C. Raman, H. Ellwood, Simon |
| author_sort | Cowley, R. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Phomopsis blight in Lupinus albus is caused by a fungal pathogen, Diaporthe toxica. It can invade all plant parts, leading to plant material becoming toxic to grazing animals, and potentially resulting in lupinosis. Identifying sources of resistance and breeding for resistance remains the best strategy for controlling Phomopsis and reducing lupinosis risks. However, loci associated with resistance to Phomopsis blight have not yet been identified. In this study, quantitative trait locus (QTL) analysis identified genomic regions associated with resistance to Phomopsis pod blight (PPB) using a linkage map of L. albus constructed previously from an F8 recombinant inbred line population derived from a cross between Kiev-Mutant (susceptible to PPB) and P27174 (resistant to PPB). Phenotyping was undertaken using a detached pod assay. In total, we identified eight QTLs for resistance to PPB on linkage group (LG) 3, LG6, LG10, LG12, LG17 and LG27 from different phenotyping environments. However, at least one QTL, QTL-5 on LG10 was consistently detected in both phenotyping environments and accounted for up to 28.2% of the total phenotypic variance. The results of this study showed that the QTL-2 on LG3 interacts epistatically with QTL-5 and QTL-6, which map on LG10 and LG12, respectively. |
| first_indexed | 2025-11-14T06:11:16Z |
| format | Journal Article |
| id | curtin-20.500.11937-6365 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:11:16Z |
| publishDate | 2014 |
| publisher | Nihon Ikushu Gakkai |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-63652017-09-13T14:40:29Z Identification of QTLs associated with resistance to Phomopsis pod blight (Diaporthe toxica) in Lupinus albus Cowley, R. Luckett, D. Ash, G. Harper, J. Vipin, C. Raman, H. Ellwood, Simon broad-leaf lupin Rqtl multi-phase experiments anamorph Phomopsis leptostromiformis DArT Phomopsis blight in Lupinus albus is caused by a fungal pathogen, Diaporthe toxica. It can invade all plant parts, leading to plant material becoming toxic to grazing animals, and potentially resulting in lupinosis. Identifying sources of resistance and breeding for resistance remains the best strategy for controlling Phomopsis and reducing lupinosis risks. However, loci associated with resistance to Phomopsis blight have not yet been identified. In this study, quantitative trait locus (QTL) analysis identified genomic regions associated with resistance to Phomopsis pod blight (PPB) using a linkage map of L. albus constructed previously from an F8 recombinant inbred line population derived from a cross between Kiev-Mutant (susceptible to PPB) and P27174 (resistant to PPB). Phenotyping was undertaken using a detached pod assay. In total, we identified eight QTLs for resistance to PPB on linkage group (LG) 3, LG6, LG10, LG12, LG17 and LG27 from different phenotyping environments. However, at least one QTL, QTL-5 on LG10 was consistently detected in both phenotyping environments and accounted for up to 28.2% of the total phenotypic variance. The results of this study showed that the QTL-2 on LG3 interacts epistatically with QTL-5 and QTL-6, which map on LG10 and LG12, respectively. 2014 Journal Article http://hdl.handle.net/20.500.11937/6365 10.1270/jsbbs.64.83 https://www.jstage.jst.go.jp/article/jsbbs/64/1/64_83/_article Nihon Ikushu Gakkai fulltext |
| spellingShingle | broad-leaf lupin Rqtl multi-phase experiments anamorph Phomopsis leptostromiformis DArT Cowley, R. Luckett, D. Ash, G. Harper, J. Vipin, C. Raman, H. Ellwood, Simon Identification of QTLs associated with resistance to Phomopsis pod blight (Diaporthe toxica) in Lupinus albus |
| title | Identification of QTLs associated with resistance to Phomopsis pod blight (Diaporthe toxica) in Lupinus albus |
| title_full | Identification of QTLs associated with resistance to Phomopsis pod blight (Diaporthe toxica) in Lupinus albus |
| title_fullStr | Identification of QTLs associated with resistance to Phomopsis pod blight (Diaporthe toxica) in Lupinus albus |
| title_full_unstemmed | Identification of QTLs associated with resistance to Phomopsis pod blight (Diaporthe toxica) in Lupinus albus |
| title_short | Identification of QTLs associated with resistance to Phomopsis pod blight (Diaporthe toxica) in Lupinus albus |
| title_sort | identification of qtls associated with resistance to phomopsis pod blight (diaporthe toxica) in lupinus albus |
| topic | broad-leaf lupin Rqtl multi-phase experiments anamorph Phomopsis leptostromiformis DArT |
| url | https://www.jstage.jst.go.jp/article/jsbbs/64/1/64_83/_article http://hdl.handle.net/20.500.11937/6365 |