Rhinovirus exacerbates house-dust-mite induced lung disease in adult mice
Human rhinovirus is a key viral trigger for asthma exacerbations. To date, murine studies investigating rhinovirus-induced exacerbation of allergic airways disease have employed systemic sensitisation/intranasal challenge with ovalbumin. In this study, we combined human-rhinovirus infection with a c...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
Public Library of Science
2014
|
| Online Access: | http://hdl.handle.net/20.500.11937/63312 |
| _version_ | 1848761053434871808 |
|---|---|
| author | Phan, J. Kicic, Anthony Berry, L. Fernandes, L. Zosky, G. Sly, P. Larcombe, A. |
| author_facet | Phan, J. Kicic, Anthony Berry, L. Fernandes, L. Zosky, G. Sly, P. Larcombe, A. |
| author_sort | Phan, J. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Human rhinovirus is a key viral trigger for asthma exacerbations. To date, murine studies investigating rhinovirus-induced exacerbation of allergic airways disease have employed systemic sensitisation/intranasal challenge with ovalbumin. In this study, we combined human-rhinovirus infection with a clinically relevant mouse model of aero-allergen exposure using house-dust-mite in an attempt to more accurately understand the links between human-rhinovirus infection and exacerbations of asthma. Adult BALB/c mice were intranasally exposed to low-dose house-dust-mite (or vehicle) daily for 10 days. On day 9, mice were inoculated with human-rhinovirus-1B (or UV-inactivated human-rhinovirus-1B). Forty-eight hours after inoculation, we assessed bronchoalveolar cellular inflammation, levels of relevant cytokines/serum antibodies, lung function and responsiveness/sensitivity to methacholine. House-dust-mite exposure did not result in a classical TH 2 -driven response, but was more representative of noneosinophilic asthma. However, there were significant effects of house-dustmite exposure on most of the parameters measured including increased cellular inflammation (primarily macrophages and neutrophils), increased total IgE and house-dust-mite-specific IgG 1 and increased responsiveness/sensitivity to methacholine. There were limited effects of human-rhinovirus-1B infection alone, and the combination of the two insults resulted in additive increases in neutrophil levels and lung parenchymal responses to methacholine (tissue elastance). We conclude that acute rhinovirus infection exacerbates house-dust-mite-induced lung disease in adult mice. The similarity of our results using the naturally occurring allergen house-dust-mite, to previous studies using ovalbumin, suggests that the exacerbation of allergic airways disease by rhinovirus infection could act via multiple or conserved mechanisms. © 2014 Phan et al. |
| first_indexed | 2025-11-14T10:25:33Z |
| format | Journal Article |
| id | curtin-20.500.11937-63312 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:25:33Z |
| publishDate | 2014 |
| publisher | Public Library of Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-633122018-02-06T06:24:17Z Rhinovirus exacerbates house-dust-mite induced lung disease in adult mice Phan, J. Kicic, Anthony Berry, L. Fernandes, L. Zosky, G. Sly, P. Larcombe, A. Human rhinovirus is a key viral trigger for asthma exacerbations. To date, murine studies investigating rhinovirus-induced exacerbation of allergic airways disease have employed systemic sensitisation/intranasal challenge with ovalbumin. In this study, we combined human-rhinovirus infection with a clinically relevant mouse model of aero-allergen exposure using house-dust-mite in an attempt to more accurately understand the links between human-rhinovirus infection and exacerbations of asthma. Adult BALB/c mice were intranasally exposed to low-dose house-dust-mite (or vehicle) daily for 10 days. On day 9, mice were inoculated with human-rhinovirus-1B (or UV-inactivated human-rhinovirus-1B). Forty-eight hours after inoculation, we assessed bronchoalveolar cellular inflammation, levels of relevant cytokines/serum antibodies, lung function and responsiveness/sensitivity to methacholine. House-dust-mite exposure did not result in a classical TH 2 -driven response, but was more representative of noneosinophilic asthma. However, there were significant effects of house-dustmite exposure on most of the parameters measured including increased cellular inflammation (primarily macrophages and neutrophils), increased total IgE and house-dust-mite-specific IgG 1 and increased responsiveness/sensitivity to methacholine. There were limited effects of human-rhinovirus-1B infection alone, and the combination of the two insults resulted in additive increases in neutrophil levels and lung parenchymal responses to methacholine (tissue elastance). We conclude that acute rhinovirus infection exacerbates house-dust-mite-induced lung disease in adult mice. The similarity of our results using the naturally occurring allergen house-dust-mite, to previous studies using ovalbumin, suggests that the exacerbation of allergic airways disease by rhinovirus infection could act via multiple or conserved mechanisms. © 2014 Phan et al. 2014 Journal Article http://hdl.handle.net/20.500.11937/63312 10.1371/journal.pone.0092163 Public Library of Science unknown |
| spellingShingle | Phan, J. Kicic, Anthony Berry, L. Fernandes, L. Zosky, G. Sly, P. Larcombe, A. Rhinovirus exacerbates house-dust-mite induced lung disease in adult mice |
| title | Rhinovirus exacerbates house-dust-mite induced lung disease in adult mice |
| title_full | Rhinovirus exacerbates house-dust-mite induced lung disease in adult mice |
| title_fullStr | Rhinovirus exacerbates house-dust-mite induced lung disease in adult mice |
| title_full_unstemmed | Rhinovirus exacerbates house-dust-mite induced lung disease in adult mice |
| title_short | Rhinovirus exacerbates house-dust-mite induced lung disease in adult mice |
| title_sort | rhinovirus exacerbates house-dust-mite induced lung disease in adult mice |
| url | http://hdl.handle.net/20.500.11937/63312 |