A Neurogenic Perspective of Sarcopenia: Time Course Study of Sciatic Nerves From Aging Mice
To elucidate the neural basis for age-related sarcopenia, we quantified morphologic and molecular changes within sciatic nerves of aging male and female C57BL/6J mice aged between 3 and 27 months using immunoblotting, immunohistochemistry, and electron microscopy. Protein analyses by immunoblotting...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
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Academic Press
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/63282 |
| _version_ | 1848761044607959040 |
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| author | Krishnan, V. White, Z. McMahon, C. Hodgetts, S. Fitzgerald, Melinda Shavlakadze, T. Harvey, A. Grounds, M. |
| author_facet | Krishnan, V. White, Z. McMahon, C. Hodgetts, S. Fitzgerald, Melinda Shavlakadze, T. Harvey, A. Grounds, M. |
| author_sort | Krishnan, V. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | To elucidate the neural basis for age-related sarcopenia, we quantified morphologic and molecular changes within sciatic nerves of aging male and female C57BL/6J mice aged between 3 and 27 months using immunoblotting, immunohistochemistry, and electron microscopy. Protein analyses by immunoblotting of nerves of male mice aged 4, 15, 18, 22, and 24 months showed increased levels of heavy chain SMI-32-positive neurofilaments, vimentin, tau5, choline acetyltransferase (ChAT), and p62 by 18–22 months. Similar protein increases were seen in 26-month-old compared with 3-month-old female mice. Immunostaining of longitudinal sections of old (27-month-old) male sciatic nerves revealed intense staining for tau5 and p62 that was increased compared with that at 3 months, but there were decreased numbers of axon profiles stained for ChAT or isolectin B4 (motor and sensory axons, respectively). Ultrastructural analysis revealed electron-dense aggregates within axons in peripheral nerves of old male mice; the proportion of axons that contained aggregates more than doubled between 15 and 27 months. Overall, the observed age-related accumulation of many proteins from about 18 months of age onward suggests impaired mechanisms for axonal transport and protein turnover. These peripheral nerve changes may contribute to the morphological and functional muscle deficits associated with sarcopenia. |
| first_indexed | 2025-11-14T10:25:25Z |
| format | Journal Article |
| id | curtin-20.500.11937-63282 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:25:25Z |
| publishDate | 2016 |
| publisher | Academic Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-632822018-04-17T08:41:40Z A Neurogenic Perspective of Sarcopenia: Time Course Study of Sciatic Nerves From Aging Mice Krishnan, V. White, Z. McMahon, C. Hodgetts, S. Fitzgerald, Melinda Shavlakadze, T. Harvey, A. Grounds, M. To elucidate the neural basis for age-related sarcopenia, we quantified morphologic and molecular changes within sciatic nerves of aging male and female C57BL/6J mice aged between 3 and 27 months using immunoblotting, immunohistochemistry, and electron microscopy. Protein analyses by immunoblotting of nerves of male mice aged 4, 15, 18, 22, and 24 months showed increased levels of heavy chain SMI-32-positive neurofilaments, vimentin, tau5, choline acetyltransferase (ChAT), and p62 by 18–22 months. Similar protein increases were seen in 26-month-old compared with 3-month-old female mice. Immunostaining of longitudinal sections of old (27-month-old) male sciatic nerves revealed intense staining for tau5 and p62 that was increased compared with that at 3 months, but there were decreased numbers of axon profiles stained for ChAT or isolectin B4 (motor and sensory axons, respectively). Ultrastructural analysis revealed electron-dense aggregates within axons in peripheral nerves of old male mice; the proportion of axons that contained aggregates more than doubled between 15 and 27 months. Overall, the observed age-related accumulation of many proteins from about 18 months of age onward suggests impaired mechanisms for axonal transport and protein turnover. These peripheral nerve changes may contribute to the morphological and functional muscle deficits associated with sarcopenia. 2016 Journal Article http://hdl.handle.net/20.500.11937/63282 10.1093/jnen/nlw019 Academic Press unknown |
| spellingShingle | Krishnan, V. White, Z. McMahon, C. Hodgetts, S. Fitzgerald, Melinda Shavlakadze, T. Harvey, A. Grounds, M. A Neurogenic Perspective of Sarcopenia: Time Course Study of Sciatic Nerves From Aging Mice |
| title | A Neurogenic Perspective of Sarcopenia: Time Course Study of Sciatic Nerves From Aging Mice |
| title_full | A Neurogenic Perspective of Sarcopenia: Time Course Study of Sciatic Nerves From Aging Mice |
| title_fullStr | A Neurogenic Perspective of Sarcopenia: Time Course Study of Sciatic Nerves From Aging Mice |
| title_full_unstemmed | A Neurogenic Perspective of Sarcopenia: Time Course Study of Sciatic Nerves From Aging Mice |
| title_short | A Neurogenic Perspective of Sarcopenia: Time Course Study of Sciatic Nerves From Aging Mice |
| title_sort | neurogenic perspective of sarcopenia: time course study of sciatic nerves from aging mice |
| url | http://hdl.handle.net/20.500.11937/63282 |