On the contributions of photorespiration and compartmentation to the contrasting intramolecular2H profiles of C3and C4plant sugars
© 2017 Elsevier Ltd Compartmentation of C 4 photosynthetic biochemistry into bundle sheath (BS) and mesophyll (M) cells, and photorespiration in C 3 plants is predicted to have hydrogen isotopic consequences for metabolites at both molecular and site-specific levels. Molecular-level evidence was rec...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
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Pergamon
2018
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| Online Access: | http://purl.org/au-research/grants/arc/DP130100577 http://hdl.handle.net/20.500.11937/63197 |
| _version_ | 1848761020325036032 |
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| author | Zhou, Y. Zhang, B. Stuart-Williams, H. Grice, Kliti Hocart, C. Gessler, A. Kayler, Z. Farquhar, G. |
| author_facet | Zhou, Y. Zhang, B. Stuart-Williams, H. Grice, Kliti Hocart, C. Gessler, A. Kayler, Z. Farquhar, G. |
| author_sort | Zhou, Y. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2017 Elsevier Ltd Compartmentation of C 4 photosynthetic biochemistry into bundle sheath (BS) and mesophyll (M) cells, and photorespiration in C 3 plants is predicted to have hydrogen isotopic consequences for metabolites at both molecular and site-specific levels. Molecular-level evidence was recently reported (Zhou et al., 2016), but evidence at the site-specific level is still lacking. We propose that such evidence exists in the contrasting 2 H distribution profiles of glucose samples from naturally grown C 3 , C 4 and CAM plants: photorespiration contributes to the relative 2 H enrichment in H 5 and relative 2 H depletion in H 1 & H 6 (the average of the two pro-chiral Hs and in particular H 6, pro-R ) in C 3 glucose, while 2 H-enriched C 3 mesophyll cellular (chloroplastic) water most likely contributes to the enrichment at H 4 ; export of (transferable hydrogen atoms of) NADPH from C 4 mesophyll cells to bundle sheath cells (via the malate shuttle) and incorporation of 2 H-relatively unenriched BS cellular water contribute to the relative depletion of H 4 & H 5 respectively; shuttling of triose-phosphates (PGA: phosphoglycerate dand DHAP: dihydroacetone phosphate) between C 4 bundle sheath and mesophyll cells contributes to the relative enrichment in H 1 & H 6 (in particular H 6, pro-R ) in C 4 glucose. |
| first_indexed | 2025-11-14T10:25:02Z |
| format | Journal Article |
| id | curtin-20.500.11937-63197 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:25:02Z |
| publishDate | 2018 |
| publisher | Pergamon |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-631972023-08-02T06:39:10Z On the contributions of photorespiration and compartmentation to the contrasting intramolecular2H profiles of C3and C4plant sugars Zhou, Y. Zhang, B. Stuart-Williams, H. Grice, Kliti Hocart, C. Gessler, A. Kayler, Z. Farquhar, G. © 2017 Elsevier Ltd Compartmentation of C 4 photosynthetic biochemistry into bundle sheath (BS) and mesophyll (M) cells, and photorespiration in C 3 plants is predicted to have hydrogen isotopic consequences for metabolites at both molecular and site-specific levels. Molecular-level evidence was recently reported (Zhou et al., 2016), but evidence at the site-specific level is still lacking. We propose that such evidence exists in the contrasting 2 H distribution profiles of glucose samples from naturally grown C 3 , C 4 and CAM plants: photorespiration contributes to the relative 2 H enrichment in H 5 and relative 2 H depletion in H 1 & H 6 (the average of the two pro-chiral Hs and in particular H 6, pro-R ) in C 3 glucose, while 2 H-enriched C 3 mesophyll cellular (chloroplastic) water most likely contributes to the enrichment at H 4 ; export of (transferable hydrogen atoms of) NADPH from C 4 mesophyll cells to bundle sheath cells (via the malate shuttle) and incorporation of 2 H-relatively unenriched BS cellular water contribute to the relative depletion of H 4 & H 5 respectively; shuttling of triose-phosphates (PGA: phosphoglycerate dand DHAP: dihydroacetone phosphate) between C 4 bundle sheath and mesophyll cells contributes to the relative enrichment in H 1 & H 6 (in particular H 6, pro-R ) in C 4 glucose. 2018 Journal Article http://hdl.handle.net/20.500.11937/63197 10.1016/j.phytochem.2017.11.004 http://purl.org/au-research/grants/arc/DP130100577 Pergamon restricted |
| spellingShingle | Zhou, Y. Zhang, B. Stuart-Williams, H. Grice, Kliti Hocart, C. Gessler, A. Kayler, Z. Farquhar, G. On the contributions of photorespiration and compartmentation to the contrasting intramolecular2H profiles of C3and C4plant sugars |
| title | On the contributions of photorespiration and compartmentation to the contrasting intramolecular2H profiles of C3and C4plant sugars |
| title_full | On the contributions of photorespiration and compartmentation to the contrasting intramolecular2H profiles of C3and C4plant sugars |
| title_fullStr | On the contributions of photorespiration and compartmentation to the contrasting intramolecular2H profiles of C3and C4plant sugars |
| title_full_unstemmed | On the contributions of photorespiration and compartmentation to the contrasting intramolecular2H profiles of C3and C4plant sugars |
| title_short | On the contributions of photorespiration and compartmentation to the contrasting intramolecular2H profiles of C3and C4plant sugars |
| title_sort | on the contributions of photorespiration and compartmentation to the contrasting intramolecular2h profiles of c3and c4plant sugars |
| url | http://purl.org/au-research/grants/arc/DP130100577 http://hdl.handle.net/20.500.11937/63197 |