Development of cryopreservation for Loxocarya cinerea: An endemic Australian plant species important for post-mining restoration
We report the development of a cryopreservation protocol for the endemic Western Australian plant species Loxocarya cinerea (Restionaceae). Shoot tips from two genotypes, SXH404 and SXH804, were cryopreserved using the droplet-vitrification technique. Control explants, which were cryoprotected, but...
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| Format: | Journal Article |
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CryoLetters
2013
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| Online Access: | http://ingentaconnect.com/content/cryo/cryo/2013/00000034/00000005;jsessionid=12rzbousixoak.alice http://hdl.handle.net/20.500.11937/6256 |
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| author | Kaczmarczyk, Anja Funnekotter, Bryn Turner, S. Bunn, E. Bryant, G. Hunt, T. Mancera, Ricardo |
| author_facet | Kaczmarczyk, Anja Funnekotter, Bryn Turner, S. Bunn, E. Bryant, G. Hunt, T. Mancera, Ricardo |
| author_sort | Kaczmarczyk, Anja |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | We report the development of a cryopreservation protocol for the endemic Western Australian plant species Loxocarya cinerea (Restionaceae). Shoot tips from two genotypes, SXH404 and SXH804, were cryopreserved using the droplet-vitrification technique. Control explants, which were cryoprotected, but not cooled, showed regeneration for both genotypes (SXH404, 22.1 ± 5.9%; SXH804, 67.7 ± 9.6%). Extension of incubation in PVS2 from 30 to 60 min did not lead to survival after cryopreservation. Thermal analysis using differential scanning calorimetry confirmed the beneficial effect of a loading phase but also revealed no or very little ice formation after cryoprotection of shoot tips in other treatments. Regeneration following cryopreservation was obtained for genotype SXH804 (4.3 ± 2.1%) but not for SXH404. Regenerated explants of L. cinerea SXH804 were morphologically identical to tissue-cultured plants. As an alternative to shoot tips, callus tissues of clone SXH404 were successfully cryopreserved (>66.7% post LN survival) using the same protocol. |
| first_indexed | 2025-11-14T06:10:46Z |
| format | Journal Article |
| id | curtin-20.500.11937-6256 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:10:46Z |
| publishDate | 2013 |
| publisher | CryoLetters |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-62562017-03-08T13:31:50Z Development of cryopreservation for Loxocarya cinerea: An endemic Australian plant species important for post-mining restoration Kaczmarczyk, Anja Funnekotter, Bryn Turner, S. Bunn, E. Bryant, G. Hunt, T. Mancera, Ricardo cryopreservation differential scanning calorimetry droplet vitrification tissue culture Loxocarya cinerea We report the development of a cryopreservation protocol for the endemic Western Australian plant species Loxocarya cinerea (Restionaceae). Shoot tips from two genotypes, SXH404 and SXH804, were cryopreserved using the droplet-vitrification technique. Control explants, which were cryoprotected, but not cooled, showed regeneration for both genotypes (SXH404, 22.1 ± 5.9%; SXH804, 67.7 ± 9.6%). Extension of incubation in PVS2 from 30 to 60 min did not lead to survival after cryopreservation. Thermal analysis using differential scanning calorimetry confirmed the beneficial effect of a loading phase but also revealed no or very little ice formation after cryoprotection of shoot tips in other treatments. Regeneration following cryopreservation was obtained for genotype SXH804 (4.3 ± 2.1%) but not for SXH404. Regenerated explants of L. cinerea SXH804 were morphologically identical to tissue-cultured plants. As an alternative to shoot tips, callus tissues of clone SXH404 were successfully cryopreserved (>66.7% post LN survival) using the same protocol. 2013 Journal Article http://hdl.handle.net/20.500.11937/6256 http://ingentaconnect.com/content/cryo/cryo/2013/00000034/00000005;jsessionid=12rzbousixoak.alice CryoLetters fulltext |
| spellingShingle | cryopreservation differential scanning calorimetry droplet vitrification tissue culture Loxocarya cinerea Kaczmarczyk, Anja Funnekotter, Bryn Turner, S. Bunn, E. Bryant, G. Hunt, T. Mancera, Ricardo Development of cryopreservation for Loxocarya cinerea: An endemic Australian plant species important for post-mining restoration |
| title | Development of cryopreservation for Loxocarya cinerea: An endemic Australian plant species important for post-mining restoration |
| title_full | Development of cryopreservation for Loxocarya cinerea: An endemic Australian plant species important for post-mining restoration |
| title_fullStr | Development of cryopreservation for Loxocarya cinerea: An endemic Australian plant species important for post-mining restoration |
| title_full_unstemmed | Development of cryopreservation for Loxocarya cinerea: An endemic Australian plant species important for post-mining restoration |
| title_short | Development of cryopreservation for Loxocarya cinerea: An endemic Australian plant species important for post-mining restoration |
| title_sort | development of cryopreservation for loxocarya cinerea: an endemic australian plant species important for post-mining restoration |
| topic | cryopreservation differential scanning calorimetry droplet vitrification tissue culture Loxocarya cinerea |
| url | http://ingentaconnect.com/content/cryo/cryo/2013/00000034/00000005;jsessionid=12rzbousixoak.alice http://hdl.handle.net/20.500.11937/6256 |