Characterization of Ag nanoparticles produced by white-rot fungi and its in vitro antimicrobial activities
Biosynthesis of Ag nanoparticles (AgNPs) with diameter ranging 50 to 80 nm is achieved using the white-rot fungi, Schizophyllum commune and Pycnoporus sanguineus. AgNPs were formed when the fungal mycelia and the supernatant reacted with AgNO 3 after 5 days of incubation period. The synthesized nan...
| Main Authors: | , |
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| Format: | Journal Article |
| Published: |
2012
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| Online Access: | http://hdl.handle.net/20.500.11937/62545 |
| _version_ | 1848760871211237376 |
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| author | Chan, Stephanie Mat Don, M. |
| author_facet | Chan, Stephanie Mat Don, M. |
| author_sort | Chan, Stephanie |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Biosynthesis of Ag nanoparticles (AgNPs) with diameter ranging 50 to 80 nm is achieved using the white-rot fungi, Schizophyllum commune and Pycnoporus sanguineus. AgNPs were formed when the fungal mycelia and the supernatant reacted with AgNO 3 after 5 days of incubation period. The synthesized nanopar-ticles were determined using analytical tools such as UV-vis spectrophotometer, and transmission electron microscopy. Results indicated that the UV-visible spectrum of the aqueous medium for S. commune and P. sanguineus showed a peak at 420 nm, which corresponded to the plasmon absorbance band of AgNPs. The antimicrobial properties of the synthesized AgNPs against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Aspergillus niger and Candida albicans were also investigated using disc diffusion assay. Minimum inhibition concentration, minimum bacterial concentration and minimum fungicidal concentration are also identifed using 96-well microtitre plate. It was found that AgNPs synthesized by the Malaysian white-rot fungi has the ability to act as an effective antibacterial agent. © Under License of Creative Commons Attribution 3.0 License. |
| first_indexed | 2025-11-14T10:22:39Z |
| format | Journal Article |
| id | curtin-20.500.11937-62545 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:22:39Z |
| publishDate | 2012 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-625452018-02-01T05:57:48Z Characterization of Ag nanoparticles produced by white-rot fungi and its in vitro antimicrobial activities Chan, Stephanie Mat Don, M. Biosynthesis of Ag nanoparticles (AgNPs) with diameter ranging 50 to 80 nm is achieved using the white-rot fungi, Schizophyllum commune and Pycnoporus sanguineus. AgNPs were formed when the fungal mycelia and the supernatant reacted with AgNO 3 after 5 days of incubation period. The synthesized nanopar-ticles were determined using analytical tools such as UV-vis spectrophotometer, and transmission electron microscopy. Results indicated that the UV-visible spectrum of the aqueous medium for S. commune and P. sanguineus showed a peak at 420 nm, which corresponded to the plasmon absorbance band of AgNPs. The antimicrobial properties of the synthesized AgNPs against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Aspergillus niger and Candida albicans were also investigated using disc diffusion assay. Minimum inhibition concentration, minimum bacterial concentration and minimum fungicidal concentration are also identifed using 96-well microtitre plate. It was found that AgNPs synthesized by the Malaysian white-rot fungi has the ability to act as an effective antibacterial agent. © Under License of Creative Commons Attribution 3.0 License. 2012 Journal Article http://hdl.handle.net/20.500.11937/62545 10.3823/717 restricted |
| spellingShingle | Chan, Stephanie Mat Don, M. Characterization of Ag nanoparticles produced by white-rot fungi and its in vitro antimicrobial activities |
| title | Characterization of Ag nanoparticles produced by white-rot fungi and its in vitro antimicrobial activities |
| title_full | Characterization of Ag nanoparticles produced by white-rot fungi and its in vitro antimicrobial activities |
| title_fullStr | Characterization of Ag nanoparticles produced by white-rot fungi and its in vitro antimicrobial activities |
| title_full_unstemmed | Characterization of Ag nanoparticles produced by white-rot fungi and its in vitro antimicrobial activities |
| title_short | Characterization of Ag nanoparticles produced by white-rot fungi and its in vitro antimicrobial activities |
| title_sort | characterization of ag nanoparticles produced by white-rot fungi and its in vitro antimicrobial activities |
| url | http://hdl.handle.net/20.500.11937/62545 |