Determination of the new anthelmintic monepantel and its sulfone metabolite in milk and muscle using a UHPLC-MS/MS and QuEChERS method
This is the first paper to report a method for the detection of the new anthelmintic monepantel and its sulfone metabolite in goat's milk and ovine muscle. Samples were extracted and purified using a modified QuEChERS method. A concentration step was included when analyzing in the low μgkg -1...
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| Format: | Journal Article |
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Elsevier BV
2011
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| Online Access: | http://hdl.handle.net/20.500.11937/61682 |
| _version_ | 1848760707421569024 |
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| author | Kinsella, Brian Byrne, P. Cantwell, H. McCormack, M. Furey, A. Danaher, M. |
| author_facet | Kinsella, Brian Byrne, P. Cantwell, H. McCormack, M. Furey, A. Danaher, M. |
| author_sort | Kinsella, Brian |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | This is the first paper to report a method for the detection of the new anthelmintic monepantel and its sulfone metabolite in goat's milk and ovine muscle. Samples were extracted and purified using a modified QuEChERS method. A concentration step was included when analyzing in the low μgkg -1 range. Analysis was carried out by ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) in a 13min run time using atmospheric pressure electrospray ionisation in the negative mode (ESI - ) and multiple reaction monitoring (MRM) scanning. Monepantel (m/z 472) and monepantel-sulfone (m/z 504) both had product ions at m/z 186 and m/z 166. The method has been single-laboratory validated according to the 2002/657/EC guidelines. The mean recovery in milk was 108 and 106% for monepantel and monepantel-sulfone, respectively. The mean recovery in muscle was 109 and 108% for monepantel and monepantel-sulfone, respectively. The coefficients of variation for the within laboratory repeatability and reproducibility were ≤6.4% in milk and ≤14.2% in muscle. The decision limits (CCα) in milk were 2.20 and 2.08μgkg -1 for monepantel and monepantel-sulfone, respectively. The decision limits (CCα) in muscle were 771 and 746μgkg -1 for monepantel and monepantel-sulfone, respectively. © 2011 Elsevier B.V. |
| first_indexed | 2025-11-14T10:20:03Z |
| format | Journal Article |
| id | curtin-20.500.11937-61682 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:20:03Z |
| publishDate | 2011 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-616822018-02-01T05:57:06Z Determination of the new anthelmintic monepantel and its sulfone metabolite in milk and muscle using a UHPLC-MS/MS and QuEChERS method Kinsella, Brian Byrne, P. Cantwell, H. McCormack, M. Furey, A. Danaher, M. This is the first paper to report a method for the detection of the new anthelmintic monepantel and its sulfone metabolite in goat's milk and ovine muscle. Samples were extracted and purified using a modified QuEChERS method. A concentration step was included when analyzing in the low μgkg -1 range. Analysis was carried out by ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) in a 13min run time using atmospheric pressure electrospray ionisation in the negative mode (ESI - ) and multiple reaction monitoring (MRM) scanning. Monepantel (m/z 472) and monepantel-sulfone (m/z 504) both had product ions at m/z 186 and m/z 166. The method has been single-laboratory validated according to the 2002/657/EC guidelines. The mean recovery in milk was 108 and 106% for monepantel and monepantel-sulfone, respectively. The mean recovery in muscle was 109 and 108% for monepantel and monepantel-sulfone, respectively. The coefficients of variation for the within laboratory repeatability and reproducibility were ≤6.4% in milk and ≤14.2% in muscle. The decision limits (CCα) in milk were 2.20 and 2.08μgkg -1 for monepantel and monepantel-sulfone, respectively. The decision limits (CCα) in muscle were 771 and 746μgkg -1 for monepantel and monepantel-sulfone, respectively. © 2011 Elsevier B.V. 2011 Journal Article http://hdl.handle.net/20.500.11937/61682 10.1016/j.jchromb.2011.10.011 Elsevier BV restricted |
| spellingShingle | Kinsella, Brian Byrne, P. Cantwell, H. McCormack, M. Furey, A. Danaher, M. Determination of the new anthelmintic monepantel and its sulfone metabolite in milk and muscle using a UHPLC-MS/MS and QuEChERS method |
| title | Determination of the new anthelmintic monepantel and its sulfone metabolite in milk and muscle using a UHPLC-MS/MS and QuEChERS method |
| title_full | Determination of the new anthelmintic monepantel and its sulfone metabolite in milk and muscle using a UHPLC-MS/MS and QuEChERS method |
| title_fullStr | Determination of the new anthelmintic monepantel and its sulfone metabolite in milk and muscle using a UHPLC-MS/MS and QuEChERS method |
| title_full_unstemmed | Determination of the new anthelmintic monepantel and its sulfone metabolite in milk and muscle using a UHPLC-MS/MS and QuEChERS method |
| title_short | Determination of the new anthelmintic monepantel and its sulfone metabolite in milk and muscle using a UHPLC-MS/MS and QuEChERS method |
| title_sort | determination of the new anthelmintic monepantel and its sulfone metabolite in milk and muscle using a uhplc-ms/ms and quechers method |
| url | http://hdl.handle.net/20.500.11937/61682 |