Decay of enteric microorganisms in biosolids-amended soil under wheat (Triticum aestivum) cultivation
There is a growing need for better assessment of health risks associated with land-applied biosolids. This study investigated in-situ decay of seeded human adenovirus (HAdV), Salmonella enterica, Escherichia coli, and bacteriophage (MS2) in biosolids-amended soil under wheat cultivation. The biosoli...
| Main Authors: | , , , , |
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| Format: | Journal Article |
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IWA Publishing
2014
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| Online Access: | http://hdl.handle.net/20.500.11937/5918 |
| _version_ | 1848744930270248960 |
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| author | Schwarz, Karen Sidhu, J. Pritchard, Deborah Li, Y. Toze, S. |
| author_facet | Schwarz, Karen Sidhu, J. Pritchard, Deborah Li, Y. Toze, S. |
| author_sort | Schwarz, Karen |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | There is a growing need for better assessment of health risks associated with land-applied biosolids. This study investigated in-situ decay of seeded human adenovirus (HAdV), Salmonella enterica, Escherichia coli, and bacteriophage (MS2) in biosolids-amended soil under wheat cultivation. The biosolids seeded with microorganisms were placed in decay chambers which were then placed in the topsoil (10 cm depth) at three different sites. Sites were selected in arid wheat-growing regions of Australia with loamy-sand soil type (Western Australia) and sandy soil (South Australia). Seeded E. coli and S. enterica had a relatively short decay time (T90 = 4–56 days) in biosolids-amended soil compared to un-amended soil (T90 = 8–83 days). The decreasing soil moisture over the wheat-growing season significantly (P < 0.05) influenced survival time of both bacteria and MS2 at Western Australia (Moora) and South Australia (Mt Compass) sites, particularly in the un-amended soils. Increasing soil temperature also significantly (P < 0.05) influenced the decay of MS2 at these sites. In this study, no notable decline in HAdV numbers (PCR detectable units) was observed in both biosolids-amended and the un-amended soils at all three sites. The HAdV decay time (T90 ≥ 180 days) in biosolids-amended and un-amended soils was significantly higher than MS2 (T90 = 22–108 days). The results of this study suggest that adenovirus could survive for a longer period of time (>180 days) during the winter in biosolids-amended soil. The stability of adenovirus suggests that consideration towards biosolids amendment frequency, time, rates and appropriate withholding periods are necessary for risk mitigation. |
| first_indexed | 2025-11-14T06:09:17Z |
| format | Journal Article |
| id | curtin-20.500.11937-5918 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:09:17Z |
| publishDate | 2014 |
| publisher | IWA Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-59182018-03-29T09:05:43Z Decay of enteric microorganisms in biosolids-amended soil under wheat (Triticum aestivum) cultivation Schwarz, Karen Sidhu, J. Pritchard, Deborah Li, Y. Toze, S. Biosolids-amended soil Salmonella enterica MS2 Pathogen decay Human adenovirus There is a growing need for better assessment of health risks associated with land-applied biosolids. This study investigated in-situ decay of seeded human adenovirus (HAdV), Salmonella enterica, Escherichia coli, and bacteriophage (MS2) in biosolids-amended soil under wheat cultivation. The biosolids seeded with microorganisms were placed in decay chambers which were then placed in the topsoil (10 cm depth) at three different sites. Sites were selected in arid wheat-growing regions of Australia with loamy-sand soil type (Western Australia) and sandy soil (South Australia). Seeded E. coli and S. enterica had a relatively short decay time (T90 = 4–56 days) in biosolids-amended soil compared to un-amended soil (T90 = 8–83 days). The decreasing soil moisture over the wheat-growing season significantly (P < 0.05) influenced survival time of both bacteria and MS2 at Western Australia (Moora) and South Australia (Mt Compass) sites, particularly in the un-amended soils. Increasing soil temperature also significantly (P < 0.05) influenced the decay of MS2 at these sites. In this study, no notable decline in HAdV numbers (PCR detectable units) was observed in both biosolids-amended and the un-amended soils at all three sites. The HAdV decay time (T90 ≥ 180 days) in biosolids-amended and un-amended soils was significantly higher than MS2 (T90 = 22–108 days). The results of this study suggest that adenovirus could survive for a longer period of time (>180 days) during the winter in biosolids-amended soil. The stability of adenovirus suggests that consideration towards biosolids amendment frequency, time, rates and appropriate withholding periods are necessary for risk mitigation. 2014 Journal Article http://hdl.handle.net/20.500.11937/5918 10.1016/j.watres.2014.03.037 IWA Publishing restricted |
| spellingShingle | Biosolids-amended soil Salmonella enterica MS2 Pathogen decay Human adenovirus Schwarz, Karen Sidhu, J. Pritchard, Deborah Li, Y. Toze, S. Decay of enteric microorganisms in biosolids-amended soil under wheat (Triticum aestivum) cultivation |
| title | Decay of enteric microorganisms in biosolids-amended soil under wheat (Triticum aestivum) cultivation |
| title_full | Decay of enteric microorganisms in biosolids-amended soil under wheat (Triticum aestivum) cultivation |
| title_fullStr | Decay of enteric microorganisms in biosolids-amended soil under wheat (Triticum aestivum) cultivation |
| title_full_unstemmed | Decay of enteric microorganisms in biosolids-amended soil under wheat (Triticum aestivum) cultivation |
| title_short | Decay of enteric microorganisms in biosolids-amended soil under wheat (Triticum aestivum) cultivation |
| title_sort | decay of enteric microorganisms in biosolids-amended soil under wheat (triticum aestivum) cultivation |
| topic | Biosolids-amended soil Salmonella enterica MS2 Pathogen decay Human adenovirus |
| url | http://hdl.handle.net/20.500.11937/5918 |