Stability of apomorphine in solutions containing selected antioxidant agents
Apomorphine in solution undergoes rapid autoxidation, producing greenish colored solutions, making it difficult to formulate as a stable pharmaceutical solution. To identify the optimum antioxidant agent/combination for apomorphine solution, a high performance liquid chromatography assay was used to...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Published: |
Dove Medical Press Ltd.
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/58960 |
| _version_ | 1848760387662512128 |
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| author | Ang, Zen Yang Boddy, Michael Liu, Yandi Sunderland, Bruce |
| author_facet | Ang, Zen Yang Boddy, Michael Liu, Yandi Sunderland, Bruce |
| author_sort | Ang, Zen Yang |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Apomorphine in solution undergoes rapid autoxidation, producing greenish colored solutions, making it difficult to formulate as a stable pharmaceutical solution. To identify the optimum antioxidant agent/combination for apomorphine solution, a high performance liquid chromatography assay was used to study the stability of 50 µg/mL apomorphine HCI in 0.1% L-ascorbic acid (AA), 0.1% sodium metabisulfite (SMB), 0.1% EDTA, and in selected combinations at 25°C, 32°C, and 37°C over a period of 14 days. The stability of apomorphine HCl (10 mg/mL) in 0.1% AA solution and in 0.1% EDTA solution at 25°C and 37°C was also evaluated. Apomorphine HCI solution (50 µg/mL) in 0.1% AA plus 0.1% SMB solution retained 99.7% (at 25°C) and 95.9% (at 37°C) of the initial concentration, as 0.1% AA plus SMB solution minimized the reactive oxygen content in solution which, in turn, reduced the oxidation rate of apomorphine HCl, and there was no green coloration perceptible. Conversely, apomorphine HCl solution (50 µg/mL) in 0.1% SMB solution was unstable as only 0.53% (at 25°C) and 0.06% (at 37°C) of the initial concentration was retained after 14 days. All 10 mg/mL apomorphine HCl samples were stable in both studies. The initial concentration of apomorphine HCl solution markedly affected its rate of oxidation and discoloration. The addition of 0.1% AA to a current formulation of apomorphine HCl injection (Apomine(®)), which contains SMB as an antioxidant, was recommended as providing the most stable solution. |
| first_indexed | 2025-11-14T10:14:58Z |
| format | Journal Article |
| id | curtin-20.500.11937-58960 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:14:58Z |
| publishDate | 2016 |
| publisher | Dove Medical Press Ltd. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-589602018-01-11T04:08:04Z Stability of apomorphine in solutions containing selected antioxidant agents Ang, Zen Yang Boddy, Michael Liu, Yandi Sunderland, Bruce Apomorphine in solution undergoes rapid autoxidation, producing greenish colored solutions, making it difficult to formulate as a stable pharmaceutical solution. To identify the optimum antioxidant agent/combination for apomorphine solution, a high performance liquid chromatography assay was used to study the stability of 50 µg/mL apomorphine HCI in 0.1% L-ascorbic acid (AA), 0.1% sodium metabisulfite (SMB), 0.1% EDTA, and in selected combinations at 25°C, 32°C, and 37°C over a period of 14 days. The stability of apomorphine HCl (10 mg/mL) in 0.1% AA solution and in 0.1% EDTA solution at 25°C and 37°C was also evaluated. Apomorphine HCI solution (50 µg/mL) in 0.1% AA plus 0.1% SMB solution retained 99.7% (at 25°C) and 95.9% (at 37°C) of the initial concentration, as 0.1% AA plus SMB solution minimized the reactive oxygen content in solution which, in turn, reduced the oxidation rate of apomorphine HCl, and there was no green coloration perceptible. Conversely, apomorphine HCl solution (50 µg/mL) in 0.1% SMB solution was unstable as only 0.53% (at 25°C) and 0.06% (at 37°C) of the initial concentration was retained after 14 days. All 10 mg/mL apomorphine HCl samples were stable in both studies. The initial concentration of apomorphine HCl solution markedly affected its rate of oxidation and discoloration. The addition of 0.1% AA to a current formulation of apomorphine HCl injection (Apomine(®)), which contains SMB as an antioxidant, was recommended as providing the most stable solution. 2016 Journal Article http://hdl.handle.net/20.500.11937/58960 10.2147/DDDT.S116848 https://creativecommons.org/licenses/by-nc/3.0/ Dove Medical Press Ltd. fulltext |
| spellingShingle | Ang, Zen Yang Boddy, Michael Liu, Yandi Sunderland, Bruce Stability of apomorphine in solutions containing selected antioxidant agents |
| title | Stability of apomorphine in solutions containing selected antioxidant agents |
| title_full | Stability of apomorphine in solutions containing selected antioxidant agents |
| title_fullStr | Stability of apomorphine in solutions containing selected antioxidant agents |
| title_full_unstemmed | Stability of apomorphine in solutions containing selected antioxidant agents |
| title_short | Stability of apomorphine in solutions containing selected antioxidant agents |
| title_sort | stability of apomorphine in solutions containing selected antioxidant agents |
| url | http://hdl.handle.net/20.500.11937/58960 |