Short-term training alters the control of mitochondrial respiration rate before maximal oxidative ATP synthesis

Aim: Short-term exercise training may induce metabolic and performance adaptations before any changes in mitochondrial enzyme potential. However, there has not been a study that has directly assessed changes in mitochondrial oxidative capacity or metabolic control as a consequence of such training i...

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Main Authors: Layec, G., Haseler, Luke, Hoff, J., Hart, C., Liu, X., Le Fur, Y., Jeong, E., Richardson, R.
Format: Journal Article
Published: 2013
Online Access:http://hdl.handle.net/20.500.11937/58360
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author Layec, G.
Haseler, Luke
Hoff, J.
Hart, C.
Liu, X.
Le Fur, Y.
Jeong, E.
Richardson, R.
author_facet Layec, G.
Haseler, Luke
Hoff, J.
Hart, C.
Liu, X.
Le Fur, Y.
Jeong, E.
Richardson, R.
author_sort Layec, G.
building Curtin Institutional Repository
collection Online Access
description Aim: Short-term exercise training may induce metabolic and performance adaptations before any changes in mitochondrial enzyme potential. However, there has not been a study that has directly assessed changes in mitochondrial oxidative capacity or metabolic control as a consequence of such training in vivo. Therefore, we used 31 P-magnetic resonance spectroscopy ( 31 P-MRS) to examine the effect of short-term plantar flexion exercise training on phosphocreatine (PCr) recovery kinetics and the control of respiration rate. Method: To this aim, we investigated 12 healthy men, experienced with this exercise modality (TRA), and 7 time-control subjects (TC). Results: After 5 days of training, maximum work rate during incremental plantar flexion exercise was significantly improved (P < 0.01). During the recovery period, the maximal rate of oxidative adenosine triphosphate synthesis (PRE: 28 ± 13 mm min -1 ; POST: 26 ± 15 mm min -1 ) and the PCr recovery time constant (PRE: 31 ± 19 s; POST: 29 ± 16) were not significantly altered. In contrast, the Hill coefficient (n H ) describing the co-operativity between respiration rate and ADP was significantly increased in TRA (PRE: n H = 2.7 ± 1.4; POST: n H = 3.4 ± 1.9, P < 0.05). Meanwhile, there were no systematic variations in any of these variables in TC. Conclusion: This study reveals that 5 days of training induces rapid adaptation in the allosteric control of respiration rate by ADP before any substantial improvement in muscle oxidative capacity occurs. © 2013 Scandinavian Physiological Society.
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spelling curtin-20.500.11937-583602018-03-29T09:08:39Z Short-term training alters the control of mitochondrial respiration rate before maximal oxidative ATP synthesis Layec, G. Haseler, Luke Hoff, J. Hart, C. Liu, X. Le Fur, Y. Jeong, E. Richardson, R. Aim: Short-term exercise training may induce metabolic and performance adaptations before any changes in mitochondrial enzyme potential. However, there has not been a study that has directly assessed changes in mitochondrial oxidative capacity or metabolic control as a consequence of such training in vivo. Therefore, we used 31 P-magnetic resonance spectroscopy ( 31 P-MRS) to examine the effect of short-term plantar flexion exercise training on phosphocreatine (PCr) recovery kinetics and the control of respiration rate. Method: To this aim, we investigated 12 healthy men, experienced with this exercise modality (TRA), and 7 time-control subjects (TC). Results: After 5 days of training, maximum work rate during incremental plantar flexion exercise was significantly improved (P < 0.01). During the recovery period, the maximal rate of oxidative adenosine triphosphate synthesis (PRE: 28 ± 13 mm min -1 ; POST: 26 ± 15 mm min -1 ) and the PCr recovery time constant (PRE: 31 ± 19 s; POST: 29 ± 16) were not significantly altered. In contrast, the Hill coefficient (n H ) describing the co-operativity between respiration rate and ADP was significantly increased in TRA (PRE: n H = 2.7 ± 1.4; POST: n H = 3.4 ± 1.9, P < 0.05). Meanwhile, there were no systematic variations in any of these variables in TC. Conclusion: This study reveals that 5 days of training induces rapid adaptation in the allosteric control of respiration rate by ADP before any substantial improvement in muscle oxidative capacity occurs. © 2013 Scandinavian Physiological Society. 2013 Journal Article http://hdl.handle.net/20.500.11937/58360 10.1111/apha.12103 restricted
spellingShingle Layec, G.
Haseler, Luke
Hoff, J.
Hart, C.
Liu, X.
Le Fur, Y.
Jeong, E.
Richardson, R.
Short-term training alters the control of mitochondrial respiration rate before maximal oxidative ATP synthesis
title Short-term training alters the control of mitochondrial respiration rate before maximal oxidative ATP synthesis
title_full Short-term training alters the control of mitochondrial respiration rate before maximal oxidative ATP synthesis
title_fullStr Short-term training alters the control of mitochondrial respiration rate before maximal oxidative ATP synthesis
title_full_unstemmed Short-term training alters the control of mitochondrial respiration rate before maximal oxidative ATP synthesis
title_short Short-term training alters the control of mitochondrial respiration rate before maximal oxidative ATP synthesis
title_sort short-term training alters the control of mitochondrial respiration rate before maximal oxidative atp synthesis
url http://hdl.handle.net/20.500.11937/58360