Determination of selected neurotransmitter metabolites using monolithic column chromatography coupled with chemiluminescence detection
The oxidation of selected clinically important neurotransmitter metabolites with acidic potassium permanganate in the presence of polyphosphates evokes chemiluminescence of sufficient intensity to enable the sensitive determination of these species. Limits of detection for 5-hydroxyindole-3-acetic a...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Published: |
Elsevier
2005
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| Online Access: | http://hdl.handle.net/20.500.11937/5812 |
| _version_ | 1848744900716134400 |
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| author | Adcock, J. Barnett, N. Costin, J. Francis, P. Lewis, Simon |
| author_facet | Adcock, J. Barnett, N. Costin, J. Francis, P. Lewis, Simon |
| author_sort | Adcock, J. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The oxidation of selected clinically important neurotransmitter metabolites with acidic potassium permanganate in the presence of polyphosphates evokes chemiluminescence of sufficient intensity to enable the sensitive determination of these species. Limits of detection for 5-hydroxyindole-3-acetic acid (5-HIAA), vanilmandelic acid (VMA; ,4-dihydroxy-3-methoxybenzeneacetic acid), 4-hydroxy-3-methoxyphenylglycol (MHPG), homovanillic acid (HVA, 4-hydroxy-3-methoxyphenylacetic acid) and 3,4-dihydroxyphenylacetic acid (DOPAC) were between 5×10-9 and 4×10-8 M, using flow-injection analysis methodology. In addition, we demonstrate the rapid determination of homovanillic acid and 5-hydroxyindole-3-acetic acid in human urine – without the need for extraction procedures – using monolithic column chromatography with chemiluminescence detection. |
| first_indexed | 2025-11-14T06:08:49Z |
| format | Journal Article |
| id | curtin-20.500.11937-5812 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:08:49Z |
| publishDate | 2005 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-58122017-09-13T14:45:48Z Determination of selected neurotransmitter metabolites using monolithic column chromatography coupled with chemiluminescence detection Adcock, J. Barnett, N. Costin, J. Francis, P. Lewis, Simon Neurotransmitter metabolites Chemiluminescence detection Monolithic column chromatography The oxidation of selected clinically important neurotransmitter metabolites with acidic potassium permanganate in the presence of polyphosphates evokes chemiluminescence of sufficient intensity to enable the sensitive determination of these species. Limits of detection for 5-hydroxyindole-3-acetic acid (5-HIAA), vanilmandelic acid (VMA; ,4-dihydroxy-3-methoxybenzeneacetic acid), 4-hydroxy-3-methoxyphenylglycol (MHPG), homovanillic acid (HVA, 4-hydroxy-3-methoxyphenylacetic acid) and 3,4-dihydroxyphenylacetic acid (DOPAC) were between 5×10-9 and 4×10-8 M, using flow-injection analysis methodology. In addition, we demonstrate the rapid determination of homovanillic acid and 5-hydroxyindole-3-acetic acid in human urine – without the need for extraction procedures – using monolithic column chromatography with chemiluminescence detection. 2005 Journal Article http://hdl.handle.net/20.500.11937/5812 10.1016/j.talanta.2005.03.007 Elsevier restricted |
| spellingShingle | Neurotransmitter metabolites Chemiluminescence detection Monolithic column chromatography Adcock, J. Barnett, N. Costin, J. Francis, P. Lewis, Simon Determination of selected neurotransmitter metabolites using monolithic column chromatography coupled with chemiluminescence detection |
| title | Determination of selected neurotransmitter metabolites using monolithic column chromatography coupled with chemiluminescence detection |
| title_full | Determination of selected neurotransmitter metabolites using monolithic column chromatography coupled with chemiluminescence detection |
| title_fullStr | Determination of selected neurotransmitter metabolites using monolithic column chromatography coupled with chemiluminescence detection |
| title_full_unstemmed | Determination of selected neurotransmitter metabolites using monolithic column chromatography coupled with chemiluminescence detection |
| title_short | Determination of selected neurotransmitter metabolites using monolithic column chromatography coupled with chemiluminescence detection |
| title_sort | determination of selected neurotransmitter metabolites using monolithic column chromatography coupled with chemiluminescence detection |
| topic | Neurotransmitter metabolites Chemiluminescence detection Monolithic column chromatography |
| url | http://hdl.handle.net/20.500.11937/5812 |