Determinants of culture success in an airway epithelium sampling program of young children with cystic fibrosis
Copyright © 2014 Informa Healthcare USA, Inc. Aim of the study: The bronchial brushing technique presents an opportunity to establish a gold standard in vitro model of Cystic Fibrosis (CF) airway disease. However, unique obstacles exist when establishing CF airway epithelial cells (pAEC < inf...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
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Taylor & Francis
2014
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| Online Access: | http://hdl.handle.net/20.500.11937/57236 |
| _version_ | 1848760029467901952 |
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| author | Garratt, L. Sutanto, E. Foo, C. Ling, K. Looi, K. Kicic-Starcevich, E. Iosifidis, T. Martinovich, K. Lannigan, F. Stick, S. Kicic, Anthony |
| author_facet | Garratt, L. Sutanto, E. Foo, C. Ling, K. Looi, K. Kicic-Starcevich, E. Iosifidis, T. Martinovich, K. Lannigan, F. Stick, S. Kicic, Anthony |
| author_sort | Garratt, L. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Copyright © 2014 Informa Healthcare USA, Inc. Aim of the study: The bronchial brushing technique presents an opportunity to establish a gold standard in vitro model of Cystic Fibrosis (CF) airway disease. However, unique obstacles exist when establishing CF airway epithelial cells (pAEC < inf > CF < /inf > ). We aimed to identify determinants of culture success through retrospective analysis of a program of routinely brushing children with CF. Materials and methods: Anaesthetised children (CF and non-CF) had airway samples taken which were immediately processed for cell culture. Airway data for the CF cohort was obtained from clinical records and the AREST CF database. Results: Of 260 brushings processed for culture, 114 (43.8%) pAEC < inf > CF < /inf > successfully cultured to passage one (P1) and 63 (24.2% of total) progressed to passage two (P2). However, > 80% of non-CF specimens (pAEC < inf > non-CF < /inf > ) cultured to P2 from similar cell numbers. Within the CF cohort, specimens successfully cultured to P2 had a higher initial cell count and lower proportion of severe CF mutation phenotype than those that did not proliferate beyond initial seeding. Elevated airway IL-8 concentration was also negatively associated with culture establishment. Contamination by opportunistic pathogens was observed in 81 (31.2% of total) cultures and brushings from children with lower respiratory tract infections were more likely to co-culture contaminating flora. Conclusions: Lower passage rates of pAEC < inf > CF < /inf > cultures uniquely contrasts with pAEC < inf > non-CF < /inf > despite similar cell numbers. An equivalent establishment rate of CF nasal epithelium reported elsewhere, significant associations to CFTR mutation phenotype, elevated airway IL-8 and opportunistic pathogens all suggest this is likely related to the CF disease milieu. |
| first_indexed | 2025-11-14T10:09:17Z |
| format | Journal Article |
| id | curtin-20.500.11937-57236 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:09:17Z |
| publishDate | 2014 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-572362017-10-30T08:35:13Z Determinants of culture success in an airway epithelium sampling program of young children with cystic fibrosis Garratt, L. Sutanto, E. Foo, C. Ling, K. Looi, K. Kicic-Starcevich, E. Iosifidis, T. Martinovich, K. Lannigan, F. Stick, S. Kicic, Anthony Copyright © 2014 Informa Healthcare USA, Inc. Aim of the study: The bronchial brushing technique presents an opportunity to establish a gold standard in vitro model of Cystic Fibrosis (CF) airway disease. However, unique obstacles exist when establishing CF airway epithelial cells (pAEC < inf > CF < /inf > ). We aimed to identify determinants of culture success through retrospective analysis of a program of routinely brushing children with CF. Materials and methods: Anaesthetised children (CF and non-CF) had airway samples taken which were immediately processed for cell culture. Airway data for the CF cohort was obtained from clinical records and the AREST CF database. Results: Of 260 brushings processed for culture, 114 (43.8%) pAEC < inf > CF < /inf > successfully cultured to passage one (P1) and 63 (24.2% of total) progressed to passage two (P2). However, > 80% of non-CF specimens (pAEC < inf > non-CF < /inf > ) cultured to P2 from similar cell numbers. Within the CF cohort, specimens successfully cultured to P2 had a higher initial cell count and lower proportion of severe CF mutation phenotype than those that did not proliferate beyond initial seeding. Elevated airway IL-8 concentration was also negatively associated with culture establishment. Contamination by opportunistic pathogens was observed in 81 (31.2% of total) cultures and brushings from children with lower respiratory tract infections were more likely to co-culture contaminating flora. Conclusions: Lower passage rates of pAEC < inf > CF < /inf > cultures uniquely contrasts with pAEC < inf > non-CF < /inf > despite similar cell numbers. An equivalent establishment rate of CF nasal epithelium reported elsewhere, significant associations to CFTR mutation phenotype, elevated airway IL-8 and opportunistic pathogens all suggest this is likely related to the CF disease milieu. 2014 Journal Article http://hdl.handle.net/20.500.11937/57236 10.3109/01902148.2014.946631 Taylor & Francis restricted |
| spellingShingle | Garratt, L. Sutanto, E. Foo, C. Ling, K. Looi, K. Kicic-Starcevich, E. Iosifidis, T. Martinovich, K. Lannigan, F. Stick, S. Kicic, Anthony Determinants of culture success in an airway epithelium sampling program of young children with cystic fibrosis |
| title | Determinants of culture success in an airway epithelium sampling program of young children with cystic fibrosis |
| title_full | Determinants of culture success in an airway epithelium sampling program of young children with cystic fibrosis |
| title_fullStr | Determinants of culture success in an airway epithelium sampling program of young children with cystic fibrosis |
| title_full_unstemmed | Determinants of culture success in an airway epithelium sampling program of young children with cystic fibrosis |
| title_short | Determinants of culture success in an airway epithelium sampling program of young children with cystic fibrosis |
| title_sort | determinants of culture success in an airway epithelium sampling program of young children with cystic fibrosis |
| url | http://hdl.handle.net/20.500.11937/57236 |