Reduced transforming growth factor ß1 (TGF-ß1) in the repair of airway epithelial cells of children with asthma
© 2016 Asian Pacific Society of Respirology Background and objective: Evidence into the role of TGF-ß1 in airway epithelial repair in asthma is still controversial. This study tested the hypothesis that the reduced TGF-ß1 levels previously observed in paediatric asthmatic airway epithelial cells dir...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
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Wiley-Blackwell Publishing Asia
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/57216 |
| _version_ | 1848760024102338560 |
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| author | Ling, K. Sutanto, E. Iosifidis, T. Kicic-Starcevich, E. Looi, K. Garratt, L. Martinovich, K. Lannigan, F. Knight, D. Stick, S. Kicic, Anthony |
| author_facet | Ling, K. Sutanto, E. Iosifidis, T. Kicic-Starcevich, E. Looi, K. Garratt, L. Martinovich, K. Lannigan, F. Knight, D. Stick, S. Kicic, Anthony |
| author_sort | Ling, K. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2016 Asian Pacific Society of Respirology Background and objective: Evidence into the role of TGF-ß1 in airway epithelial repair in asthma is still controversial. This study tested the hypothesis that the reduced TGF-ß1 levels previously observed in paediatric asthmatic airway epithelial cells directly contribute to the dysregulated repair seen in these cells. Methods: Primary airway epithelial cells (pAEC) from children with asthma (n = 16) and non-asthmatic subjects (n = 20) were isolated, and subcultured for investigation of TGF-ß1 gene and protein via quantitative polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Expression of other associated genes such as integrins avß6, avß8 and MT1-MMP were also tested. Small interfering RNA (siRNA) was employed to assess the role of TGF-ß1 during wound repair. Results: TGF-ß1 gene and protein expression were significantly downregulated in asthmatic pAEC over the course of repair, compared with cells from non-asthmatic children. Messenger RNA (mRNA) expression of TGF-ß1 was also directly implicated in non-asthmatic and asthmatic pAEC proliferation over their quiescent counterparts. Small interfering RNA-mediated knockdown of TGF-ß1 compromised repair in non-asthmatic pAEC and exacerbated the dysregulated repair seen in asthmatic pAEC. Expression of major TGF-ß1 activators of epithelial cells, integrin avß6 and avß8 was also measured and there was no difference in avß6 gene expression between the two cohorts. Although integrin avß8 gene expression was significantly higher in asthmatic pAEC, the expression of MT1-MMP (MMP14) which facilitates the avß8 mediated TGF-ß1 activation was significantly downregulated. Conclusion: Our data has highlighted the importance of TGF-ß1 in pAEC wound repair in vitro. The significantly lower levels seen in asthmatic pAEC subsequently contributes to the dysregulated repair observed in these cells. |
| first_indexed | 2025-11-14T10:09:12Z |
| format | Journal Article |
| id | curtin-20.500.11937-57216 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:09:12Z |
| publishDate | 2016 |
| publisher | Wiley-Blackwell Publishing Asia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-572162017-10-30T08:35:19Z Reduced transforming growth factor ß1 (TGF-ß1) in the repair of airway epithelial cells of children with asthma Ling, K. Sutanto, E. Iosifidis, T. Kicic-Starcevich, E. Looi, K. Garratt, L. Martinovich, K. Lannigan, F. Knight, D. Stick, S. Kicic, Anthony © 2016 Asian Pacific Society of Respirology Background and objective: Evidence into the role of TGF-ß1 in airway epithelial repair in asthma is still controversial. This study tested the hypothesis that the reduced TGF-ß1 levels previously observed in paediatric asthmatic airway epithelial cells directly contribute to the dysregulated repair seen in these cells. Methods: Primary airway epithelial cells (pAEC) from children with asthma (n = 16) and non-asthmatic subjects (n = 20) were isolated, and subcultured for investigation of TGF-ß1 gene and protein via quantitative polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Expression of other associated genes such as integrins avß6, avß8 and MT1-MMP were also tested. Small interfering RNA (siRNA) was employed to assess the role of TGF-ß1 during wound repair. Results: TGF-ß1 gene and protein expression were significantly downregulated in asthmatic pAEC over the course of repair, compared with cells from non-asthmatic children. Messenger RNA (mRNA) expression of TGF-ß1 was also directly implicated in non-asthmatic and asthmatic pAEC proliferation over their quiescent counterparts. Small interfering RNA-mediated knockdown of TGF-ß1 compromised repair in non-asthmatic pAEC and exacerbated the dysregulated repair seen in asthmatic pAEC. Expression of major TGF-ß1 activators of epithelial cells, integrin avß6 and avß8 was also measured and there was no difference in avß6 gene expression between the two cohorts. Although integrin avß8 gene expression was significantly higher in asthmatic pAEC, the expression of MT1-MMP (MMP14) which facilitates the avß8 mediated TGF-ß1 activation was significantly downregulated. Conclusion: Our data has highlighted the importance of TGF-ß1 in pAEC wound repair in vitro. The significantly lower levels seen in asthmatic pAEC subsequently contributes to the dysregulated repair observed in these cells. 2016 Journal Article http://hdl.handle.net/20.500.11937/57216 10.1111/resp.12810 Wiley-Blackwell Publishing Asia unknown |
| spellingShingle | Ling, K. Sutanto, E. Iosifidis, T. Kicic-Starcevich, E. Looi, K. Garratt, L. Martinovich, K. Lannigan, F. Knight, D. Stick, S. Kicic, Anthony Reduced transforming growth factor ß1 (TGF-ß1) in the repair of airway epithelial cells of children with asthma |
| title | Reduced transforming growth factor ß1 (TGF-ß1) in the repair of airway epithelial cells of children with asthma |
| title_full | Reduced transforming growth factor ß1 (TGF-ß1) in the repair of airway epithelial cells of children with asthma |
| title_fullStr | Reduced transforming growth factor ß1 (TGF-ß1) in the repair of airway epithelial cells of children with asthma |
| title_full_unstemmed | Reduced transforming growth factor ß1 (TGF-ß1) in the repair of airway epithelial cells of children with asthma |
| title_short | Reduced transforming growth factor ß1 (TGF-ß1) in the repair of airway epithelial cells of children with asthma |
| title_sort | reduced transforming growth factor ß1 (tgf-ß1) in the repair of airway epithelial cells of children with asthma |
| url | http://hdl.handle.net/20.500.11937/57216 |