Impaired airway epithelial cell responses from children with asthma to rhinoviral infection
© 2016 John Wiley & Sons Ltd Background: The airway epithelium forms an effective immune and physical barrier that is essential for protecting the lung from potentially harmful inhaled stimuli including viruses. Human rhinovirus (HRV) infection is a known trigger of asthma exacerbations, altho...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Journal Article |
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Blackwell Science Ltd
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/57189 |
| _version_ | 1848760016554688512 |
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| author | Kicic, Anthony Stevens, P. Sutanto, E. Kicic-Starcevich, E. Ling, K. Looi, K. Martinovich, K. Garratt, L. Iosifidis, T. Shaw, N. Buckley, A. Rigby, P. Lannigan, F. Knight, D. Stick, S. |
| author_facet | Kicic, Anthony Stevens, P. Sutanto, E. Kicic-Starcevich, E. Ling, K. Looi, K. Martinovich, K. Garratt, L. Iosifidis, T. Shaw, N. Buckley, A. Rigby, P. Lannigan, F. Knight, D. Stick, S. |
| author_sort | Kicic, Anthony |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2016 John Wiley & Sons Ltd Background: The airway epithelium forms an effective immune and physical barrier that is essential for protecting the lung from potentially harmful inhaled stimuli including viruses. Human rhinovirus (HRV) infection is a known trigger of asthma exacerbations, although the mechanism by which this occurs is not fully understood. Objective: To explore the relationship between apoptotic, innate immune and inflammatory responses to HRV infection in airway epithelial cells (AECs) obtained from children with asthma and non-asthmatic controls. In addition, to test the hypothesis that aberrant repair of epithelium from asthmatics is further dysregulated by HRV infection. Methods: Airway epithelial brushings were obtained from 39 asthmatic and 36 non-asthmatic children. Primary cultures were established and exposed to HRV1b and HRV14. Virus receptor number, virus replication and progeny release were determined. Epithelial cell apoptosis, IFN-ß production, inflammatory cytokine release and epithelial wound repair and proliferation were also measured. Results: Virus proliferation and release was greater in airway epithelial cells from asthmatics but this was not related to the number of virus receptors. In epithelial cells from asthmatic children, virus infection dampened apoptosis, reduced IFN-ß production and increased inflammatory cytokine production. HRV1b infection also inhibited wound repair capacity of epithelial cells isolated from non-asthmatic children and exaggerated the defective repair response seen in epithelial cells from asthmatics. Addition of IFN-ß restored apoptosis, suppressed virus replication and improved repair of airway epithelial cells from asthmatics but did not reduce inflammatory cytokine production. Conclusions: Collectively, HRV infection delays repair and inhibits apoptotic processes in epithelial cells from non-asthmatic and asthmatic children. The delayed repair is further exaggerated in cells from asthmatic children and is only partially reversed by exogenous IFN-ß. |
| first_indexed | 2025-11-14T10:09:04Z |
| format | Journal Article |
| id | curtin-20.500.11937-57189 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:09:04Z |
| publishDate | 2016 |
| publisher | Blackwell Science Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-571892017-10-30T08:35:13Z Impaired airway epithelial cell responses from children with asthma to rhinoviral infection Kicic, Anthony Stevens, P. Sutanto, E. Kicic-Starcevich, E. Ling, K. Looi, K. Martinovich, K. Garratt, L. Iosifidis, T. Shaw, N. Buckley, A. Rigby, P. Lannigan, F. Knight, D. Stick, S. © 2016 John Wiley & Sons Ltd Background: The airway epithelium forms an effective immune and physical barrier that is essential for protecting the lung from potentially harmful inhaled stimuli including viruses. Human rhinovirus (HRV) infection is a known trigger of asthma exacerbations, although the mechanism by which this occurs is not fully understood. Objective: To explore the relationship between apoptotic, innate immune and inflammatory responses to HRV infection in airway epithelial cells (AECs) obtained from children with asthma and non-asthmatic controls. In addition, to test the hypothesis that aberrant repair of epithelium from asthmatics is further dysregulated by HRV infection. Methods: Airway epithelial brushings were obtained from 39 asthmatic and 36 non-asthmatic children. Primary cultures were established and exposed to HRV1b and HRV14. Virus receptor number, virus replication and progeny release were determined. Epithelial cell apoptosis, IFN-ß production, inflammatory cytokine release and epithelial wound repair and proliferation were also measured. Results: Virus proliferation and release was greater in airway epithelial cells from asthmatics but this was not related to the number of virus receptors. In epithelial cells from asthmatic children, virus infection dampened apoptosis, reduced IFN-ß production and increased inflammatory cytokine production. HRV1b infection also inhibited wound repair capacity of epithelial cells isolated from non-asthmatic children and exaggerated the defective repair response seen in epithelial cells from asthmatics. Addition of IFN-ß restored apoptosis, suppressed virus replication and improved repair of airway epithelial cells from asthmatics but did not reduce inflammatory cytokine production. Conclusions: Collectively, HRV infection delays repair and inhibits apoptotic processes in epithelial cells from non-asthmatic and asthmatic children. The delayed repair is further exaggerated in cells from asthmatic children and is only partially reversed by exogenous IFN-ß. 2016 Journal Article http://hdl.handle.net/20.500.11937/57189 10.1111/cea.12767 Blackwell Science Ltd restricted |
| spellingShingle | Kicic, Anthony Stevens, P. Sutanto, E. Kicic-Starcevich, E. Ling, K. Looi, K. Martinovich, K. Garratt, L. Iosifidis, T. Shaw, N. Buckley, A. Rigby, P. Lannigan, F. Knight, D. Stick, S. Impaired airway epithelial cell responses from children with asthma to rhinoviral infection |
| title | Impaired airway epithelial cell responses from children with asthma to rhinoviral infection |
| title_full | Impaired airway epithelial cell responses from children with asthma to rhinoviral infection |
| title_fullStr | Impaired airway epithelial cell responses from children with asthma to rhinoviral infection |
| title_full_unstemmed | Impaired airway epithelial cell responses from children with asthma to rhinoviral infection |
| title_short | Impaired airway epithelial cell responses from children with asthma to rhinoviral infection |
| title_sort | impaired airway epithelial cell responses from children with asthma to rhinoviral infection |
| url | http://hdl.handle.net/20.500.11937/57189 |