Advances in understanding the fundamental aspects required for successful cryopreservation of Australian flora
Australia is host to an amazing diversity of species, many of which require conservation efforts. In vitro culture provides a tool for not only conserving these threatened species but allows for their propagation from limited starting material. Cryopreservation provides the greatest long-term storag...
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| Format: | Journal Article |
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Springer
2017
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| Online Access: | http://purl.org/au-research/grants/arc/LP140100993 http://hdl.handle.net/20.500.11937/56947 |
| _version_ | 1848759977030713344 |
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| author | Funnekotter, Bryn Mancera, Ricardo Bunn, E. |
| author_facet | Funnekotter, Bryn Mancera, Ricardo Bunn, E. |
| author_sort | Funnekotter, Bryn |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Australia is host to an amazing diversity of species, many of which require conservation efforts. In vitro culture provides a tool for not only conserving these threatened species but allows for their propagation from limited starting material. Cryopreservation provides the greatest long-term storage option for in vitro cultures and as a conservation tool for other germplasm. However, while cryopreservation has proven capable of delivering viable long-term storage with some plant taxa, the process of deriving protocols is still largely an incremental process. The key to faster and more intuitive optimising of cryopreservation protocols lies with continuing to develop a better understanding of key factors, including issues with plant physiology (such as genetic stability, the composition of the proteome and metabolome, cell membrane characteristics, and antioxidant defences) and how the stresses imposed by cryopreservation (such as the excision damage, desiccation, cryoprotective agent toxicity, ice crystal damage, and cooling to cryogenic temperatures) interact and contribute to the cryocapability of a species. This review focuses on the advances that have been made towards understanding cryogenic stress and how this has led to improved cryopreservation protocols, in the context of cryopreserving Australian flora. |
| first_indexed | 2025-11-14T10:08:27Z |
| format | Journal Article |
| id | curtin-20.500.11937-56947 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:08:27Z |
| publishDate | 2017 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-569472023-02-02T03:24:11Z Advances in understanding the fundamental aspects required for successful cryopreservation of Australian flora Funnekotter, Bryn Mancera, Ricardo Bunn, E. Australia is host to an amazing diversity of species, many of which require conservation efforts. In vitro culture provides a tool for not only conserving these threatened species but allows for their propagation from limited starting material. Cryopreservation provides the greatest long-term storage option for in vitro cultures and as a conservation tool for other germplasm. However, while cryopreservation has proven capable of delivering viable long-term storage with some plant taxa, the process of deriving protocols is still largely an incremental process. The key to faster and more intuitive optimising of cryopreservation protocols lies with continuing to develop a better understanding of key factors, including issues with plant physiology (such as genetic stability, the composition of the proteome and metabolome, cell membrane characteristics, and antioxidant defences) and how the stresses imposed by cryopreservation (such as the excision damage, desiccation, cryoprotective agent toxicity, ice crystal damage, and cooling to cryogenic temperatures) interact and contribute to the cryocapability of a species. This review focuses on the advances that have been made towards understanding cryogenic stress and how this has led to improved cryopreservation protocols, in the context of cryopreserving Australian flora. 2017 Journal Article http://hdl.handle.net/20.500.11937/56947 10.1007/s11627-017-9850-5 http://purl.org/au-research/grants/arc/LP140100993 Springer restricted |
| spellingShingle | Funnekotter, Bryn Mancera, Ricardo Bunn, E. Advances in understanding the fundamental aspects required for successful cryopreservation of Australian flora |
| title | Advances in understanding the fundamental aspects required for successful cryopreservation of Australian flora |
| title_full | Advances in understanding the fundamental aspects required for successful cryopreservation of Australian flora |
| title_fullStr | Advances in understanding the fundamental aspects required for successful cryopreservation of Australian flora |
| title_full_unstemmed | Advances in understanding the fundamental aspects required for successful cryopreservation of Australian flora |
| title_short | Advances in understanding the fundamental aspects required for successful cryopreservation of Australian flora |
| title_sort | advances in understanding the fundamental aspects required for successful cryopreservation of australian flora |
| url | http://purl.org/au-research/grants/arc/LP140100993 http://hdl.handle.net/20.500.11937/56947 |