CMV drives the expansion of highly functional memory T cells expressing NK-cell receptors in renal transplant recipients
Cytomegalovirus (CMV) is a common opportunistic infection encountered in renal transplant recipients (RTRs) and may be reactivated without symptoms at any time post-transplant. We describe how active and latent CMV affect T-cell subsets in RTRs who are stable on maintenance therapy. T-cell responses...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
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Wiley-VCH Verlag GmbH & Co. KGaA
2017
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| Online Access: | http://hdl.handle.net/20.500.11937/56920 |
| _version_ | 1848759969633009664 |
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| author | Makwana, N. Foley, B. Fernandez, S. Lee, S. Irish, A. Pircher, H. Price, Patricia |
| author_facet | Makwana, N. Foley, B. Fernandez, S. Lee, S. Irish, A. Pircher, H. Price, Patricia |
| author_sort | Makwana, N. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Cytomegalovirus (CMV) is a common opportunistic infection encountered in renal transplant recipients (RTRs) and may be reactivated without symptoms at any time post-transplant. We describe how active and latent CMV affect T-cell subsets in RTRs who are stable on maintenance therapy. T-cell responses to CMV were assessed in RTRs (n = 54) > 2 years post-transplant, and healthy controls (n = 38). Seven RTRs had CMV DNA detectable in plasma. CMV antibody and DNA aligned with increased proportions of CD8 + T cells and reduced CD4/CD8 ratios. This paralleled an expansion of effector memory T-cell (T EM ), terminally differentiated T-cell (T EMRA ) and CD57 + T EMRA cell populations. Expression of NK-cell receptors, LIR-1 and KLRG1 on CD4 + and CD8 + CD57 + T EM and T EMRA cells correlated with elevated interferon-? and cytotoxic responses to anti-CD3 and increased cytotoxic responses to CMV phosphoprotein (pp) 65 in RTRs who carried CMV DNA. CD8 + T cells from all CMV seropositive RTRs responded efficiently to CMV immediate early (IE) -1 peptides. The data show that latent and active CMV infection can alter T-cell subsets in RTRs many years after transplantation, and up-regulate T-cell expression of NK-cell receptors. This may enhance effector responses of CD4 + and CD8 + T cells against CMV. |
| first_indexed | 2025-11-14T10:08:20Z |
| format | Journal Article |
| id | curtin-20.500.11937-56920 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:08:20Z |
| publishDate | 2017 |
| publisher | Wiley-VCH Verlag GmbH & Co. KGaA |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-569202018-07-10T01:45:46Z CMV drives the expansion of highly functional memory T cells expressing NK-cell receptors in renal transplant recipients Makwana, N. Foley, B. Fernandez, S. Lee, S. Irish, A. Pircher, H. Price, Patricia Cytomegalovirus (CMV) is a common opportunistic infection encountered in renal transplant recipients (RTRs) and may be reactivated without symptoms at any time post-transplant. We describe how active and latent CMV affect T-cell subsets in RTRs who are stable on maintenance therapy. T-cell responses to CMV were assessed in RTRs (n = 54) > 2 years post-transplant, and healthy controls (n = 38). Seven RTRs had CMV DNA detectable in plasma. CMV antibody and DNA aligned with increased proportions of CD8 + T cells and reduced CD4/CD8 ratios. This paralleled an expansion of effector memory T-cell (T EM ), terminally differentiated T-cell (T EMRA ) and CD57 + T EMRA cell populations. Expression of NK-cell receptors, LIR-1 and KLRG1 on CD4 + and CD8 + CD57 + T EM and T EMRA cells correlated with elevated interferon-? and cytotoxic responses to anti-CD3 and increased cytotoxic responses to CMV phosphoprotein (pp) 65 in RTRs who carried CMV DNA. CD8 + T cells from all CMV seropositive RTRs responded efficiently to CMV immediate early (IE) -1 peptides. The data show that latent and active CMV infection can alter T-cell subsets in RTRs many years after transplantation, and up-regulate T-cell expression of NK-cell receptors. This may enhance effector responses of CD4 + and CD8 + T cells against CMV. 2017 Journal Article http://hdl.handle.net/20.500.11937/56920 10.1002/eji.201747018 Wiley-VCH Verlag GmbH & Co. KGaA fulltext |
| spellingShingle | Makwana, N. Foley, B. Fernandez, S. Lee, S. Irish, A. Pircher, H. Price, Patricia CMV drives the expansion of highly functional memory T cells expressing NK-cell receptors in renal transplant recipients |
| title | CMV drives the expansion of highly functional memory T cells expressing NK-cell receptors in renal transplant recipients |
| title_full | CMV drives the expansion of highly functional memory T cells expressing NK-cell receptors in renal transplant recipients |
| title_fullStr | CMV drives the expansion of highly functional memory T cells expressing NK-cell receptors in renal transplant recipients |
| title_full_unstemmed | CMV drives the expansion of highly functional memory T cells expressing NK-cell receptors in renal transplant recipients |
| title_short | CMV drives the expansion of highly functional memory T cells expressing NK-cell receptors in renal transplant recipients |
| title_sort | cmv drives the expansion of highly functional memory t cells expressing nk-cell receptors in renal transplant recipients |
| url | http://hdl.handle.net/20.500.11937/56920 |