Polydopamine-assisted decoration of TiO2 nanotube arrays with enzyme to construct a novel photoelectrochemical sensing platform

A novel photoelectrochemical (PEC) bios ensing platform coupling with enzyme-induced biocatalytic precipitation (BCP) for H 2 O 2 was successfully constructed. TiO 2 nanotube arrays (TNs) were coated with polydopamine (PDA) and horseradish peroxidase (HRP) as the PEC electrode through a facile met...

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Main Authors: Li, J., Li, X., Zhao, Q., Jiang, Z., Tade, Moses, Wang, S., Liu, Shaomin
Format: Journal Article
Published: Elsevier 2018
Online Access:http://hdl.handle.net/20.500.11937/56867
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author Li, J.
Li, X.
Zhao, Q.
Jiang, Z.
Tade, Moses
Wang, S.
Liu, Shaomin
author_facet Li, J.
Li, X.
Zhao, Q.
Jiang, Z.
Tade, Moses
Wang, S.
Liu, Shaomin
author_sort Li, J.
building Curtin Institutional Repository
collection Online Access
description A novel photoelectrochemical (PEC) bios ensing platform coupling with enzyme-induced biocatalytic precipitation (BCP) for H 2 O 2 was successfully constructed. TiO 2 nanotube arrays (TNs) were coated with polydopamine (PDA) and horseradish peroxidase (HRP) as the PEC electrode through a facile method. PDA was incorporated into the sensor system not only as a sensitizing agent to alter the optical properties of TiO 2 nanotube arrays and enhance the visible light (? > 400 nm) absorption, but also as a bonding resin to HRP via Michael addition or Schiff base reaction. The immobilized HRP on the PEC electrodes could induce BCP reaction as the signal amplifier by oxidizing the 4-chloro-1-naphthol (4-CN) into insoluble products in the presence of H 2 O 2 . The quantitative monitoring of H 2 O 2 could be realized thanks to the photocurrent decrease caused by the BCP generated insoluble products deposited on the PEC electrode surface. The fabricated TNs@HRP/PDA PEC electrode for biosensing of H 2 O 2 exhibited a good sensitivity as well as selectivity in a detection range from 1 nM to 50 µM, with a detection limit of 0.7 nM.
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institution Curtin University Malaysia
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publishDate 2018
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spelling curtin-20.500.11937-568672018-01-17T04:58:54Z Polydopamine-assisted decoration of TiO2 nanotube arrays with enzyme to construct a novel photoelectrochemical sensing platform Li, J. Li, X. Zhao, Q. Jiang, Z. Tade, Moses Wang, S. Liu, Shaomin A novel photoelectrochemical (PEC) bios ensing platform coupling with enzyme-induced biocatalytic precipitation (BCP) for H 2 O 2 was successfully constructed. TiO 2 nanotube arrays (TNs) were coated with polydopamine (PDA) and horseradish peroxidase (HRP) as the PEC electrode through a facile method. PDA was incorporated into the sensor system not only as a sensitizing agent to alter the optical properties of TiO 2 nanotube arrays and enhance the visible light (? > 400 nm) absorption, but also as a bonding resin to HRP via Michael addition or Schiff base reaction. The immobilized HRP on the PEC electrodes could induce BCP reaction as the signal amplifier by oxidizing the 4-chloro-1-naphthol (4-CN) into insoluble products in the presence of H 2 O 2 . The quantitative monitoring of H 2 O 2 could be realized thanks to the photocurrent decrease caused by the BCP generated insoluble products deposited on the PEC electrode surface. The fabricated TNs@HRP/PDA PEC electrode for biosensing of H 2 O 2 exhibited a good sensitivity as well as selectivity in a detection range from 1 nM to 50 µM, with a detection limit of 0.7 nM. 2018 Journal Article http://hdl.handle.net/20.500.11937/56867 10.1016/j.snb.2017.06.168 Elsevier restricted
spellingShingle Li, J.
Li, X.
Zhao, Q.
Jiang, Z.
Tade, Moses
Wang, S.
Liu, Shaomin
Polydopamine-assisted decoration of TiO2 nanotube arrays with enzyme to construct a novel photoelectrochemical sensing platform
title Polydopamine-assisted decoration of TiO2 nanotube arrays with enzyme to construct a novel photoelectrochemical sensing platform
title_full Polydopamine-assisted decoration of TiO2 nanotube arrays with enzyme to construct a novel photoelectrochemical sensing platform
title_fullStr Polydopamine-assisted decoration of TiO2 nanotube arrays with enzyme to construct a novel photoelectrochemical sensing platform
title_full_unstemmed Polydopamine-assisted decoration of TiO2 nanotube arrays with enzyme to construct a novel photoelectrochemical sensing platform
title_short Polydopamine-assisted decoration of TiO2 nanotube arrays with enzyme to construct a novel photoelectrochemical sensing platform
title_sort polydopamine-assisted decoration of tio2 nanotube arrays with enzyme to construct a novel photoelectrochemical sensing platform
url http://hdl.handle.net/20.500.11937/56867