Conventional and In Vitro Propagation of Lechenaultia macrantha (Goodeniaceae)

Lechenaultia macrantha K. Krause (Goodeniaceae) is a species with great horticultural potential that is endemic to the sandy and gravelly soils of central, southwestern Australia. The effectiveness of several conventional and in vitro propagation techniques were assessed. Seeds possessed non-deep ph...

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Main Authors: Png, G., Downes, Katherine, Tan, B.
Format: Journal Article
Published: American Society for Horticultural Science 2012
Subjects:
Online Access:http://hortsci.ashspublications.org/content/48/1/108.full
http://hdl.handle.net/20.500.11937/5668
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author Png, G.
Downes, Katherine
Tan, B.
author_facet Png, G.
Downes, Katherine
Tan, B.
author_sort Png, G.
building Curtin Institutional Repository
collection Online Access
description Lechenaultia macrantha K. Krause (Goodeniaceae) is a species with great horticultural potential that is endemic to the sandy and gravelly soils of central, southwestern Australia. The effectiveness of several conventional and in vitro propagation techniques were assessed. Seeds possessed non-deep physiological dormancy, and a combination of seed nicking and imbibition in 10% (v/v) smoke water for 24 hours resulted in 81% germination after 33 days of incubation. Softwood stem cuttings produced adventitious roots readily without the need for exogenous auxin application. In vitro microcuttings produced shoots in response to cytokinins. However, optimal or suboptimal cytokinin concentrations resulted in hyperhydric shoots, which poses an obstacle to successful micropropagation. The use of auxin was necessary for the initiation of adventitious roots in vitro. This information will assist in the horticultural development of this species.
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institution Curtin University Malaysia
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publishDate 2012
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spelling curtin-20.500.11937-56682017-01-30T10:47:43Z Conventional and In Vitro Propagation of Lechenaultia macrantha (Goodeniaceae) Png, G. Downes, Katherine Tan, B. stem cuttings micropropagation seed germination ornamental Australian endemic dormancy tissue culture Lechenaultia macrantha K. Krause (Goodeniaceae) is a species with great horticultural potential that is endemic to the sandy and gravelly soils of central, southwestern Australia. The effectiveness of several conventional and in vitro propagation techniques were assessed. Seeds possessed non-deep physiological dormancy, and a combination of seed nicking and imbibition in 10% (v/v) smoke water for 24 hours resulted in 81% germination after 33 days of incubation. Softwood stem cuttings produced adventitious roots readily without the need for exogenous auxin application. In vitro microcuttings produced shoots in response to cytokinins. However, optimal or suboptimal cytokinin concentrations resulted in hyperhydric shoots, which poses an obstacle to successful micropropagation. The use of auxin was necessary for the initiation of adventitious roots in vitro. This information will assist in the horticultural development of this species. 2012 Journal Article http://hdl.handle.net/20.500.11937/5668 http://hortsci.ashspublications.org/content/48/1/108.full American Society for Horticultural Science restricted
spellingShingle stem cuttings
micropropagation
seed germination
ornamental Australian endemic
dormancy
tissue culture
Png, G.
Downes, Katherine
Tan, B.
Conventional and In Vitro Propagation of Lechenaultia macrantha (Goodeniaceae)
title Conventional and In Vitro Propagation of Lechenaultia macrantha (Goodeniaceae)
title_full Conventional and In Vitro Propagation of Lechenaultia macrantha (Goodeniaceae)
title_fullStr Conventional and In Vitro Propagation of Lechenaultia macrantha (Goodeniaceae)
title_full_unstemmed Conventional and In Vitro Propagation of Lechenaultia macrantha (Goodeniaceae)
title_short Conventional and In Vitro Propagation of Lechenaultia macrantha (Goodeniaceae)
title_sort conventional and in vitro propagation of lechenaultia macrantha (goodeniaceae)
topic stem cuttings
micropropagation
seed germination
ornamental Australian endemic
dormancy
tissue culture
url http://hortsci.ashspublications.org/content/48/1/108.full
http://hdl.handle.net/20.500.11937/5668