Myb via TGFβ is required for collagen type 1 production and skin integrity
Skin integrity requires an ongoing replacement and repair orchestrated by several cell types. We previously investigated the architecture of the skin of avian myeloblastosis viral oncogene homolog (Myb) knock-out (KO) embryos and wound repair in Myb+/− mice revealing a need for Myb in the skin, attr...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
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JAI Press
2015
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| Online Access: | http://hdl.handle.net/20.500.11937/56640 |
| _version_ | 1848759902861787136 |
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| author | Sampurno, S. Cross, R. Pearson, H. Kaur, Pritinder Malaterre, J. Ramsay, R. |
| author_facet | Sampurno, S. Cross, R. Pearson, H. Kaur, Pritinder Malaterre, J. Ramsay, R. |
| author_sort | Sampurno, S. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Skin integrity requires an ongoing replacement and repair orchestrated by several cell types. We previously investigated the architecture of the skin of avian myeloblastosis viral oncogene homolog (Myb) knock-out (KO) embryos and wound repair in Myb+/− mice revealing a need for Myb in the skin, attributed to fibroblast-dependent production of collagen type 1. Here, using targeted Myb deletion in keratin-14 (K14) positive cells we reveal further Myb-specific defects in epidermal cell proliferation, thickness and ultrastructural morphology. This was associated with a severe deficit in collagen type 1 production, reminiscent of that observed in patients with ichthyosis vulgaris and Ehlers–Danlos syndrome. Since collagen type 1 is a product of fibroblasts, the collagen defect observed was unexpected and appears to be directed by the loss of Myb with significantly reduced tumor growth factor beta 1 (Tgfβ−1) expression by primary keratinocytes. Our findings support a specific role for Myb in K14+ epithelial cells in the preservation of adult skin integrity and function. |
| first_indexed | 2025-11-14T10:07:16Z |
| format | Journal Article |
| id | curtin-20.500.11937-56640 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:07:16Z |
| publishDate | 2015 |
| publisher | JAI Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-566402018-01-09T01:55:55Z Myb via TGFβ is required for collagen type 1 production and skin integrity Sampurno, S. Cross, R. Pearson, H. Kaur, Pritinder Malaterre, J. Ramsay, R. Skin integrity requires an ongoing replacement and repair orchestrated by several cell types. We previously investigated the architecture of the skin of avian myeloblastosis viral oncogene homolog (Myb) knock-out (KO) embryos and wound repair in Myb+/− mice revealing a need for Myb in the skin, attributed to fibroblast-dependent production of collagen type 1. Here, using targeted Myb deletion in keratin-14 (K14) positive cells we reveal further Myb-specific defects in epidermal cell proliferation, thickness and ultrastructural morphology. This was associated with a severe deficit in collagen type 1 production, reminiscent of that observed in patients with ichthyosis vulgaris and Ehlers–Danlos syndrome. Since collagen type 1 is a product of fibroblasts, the collagen defect observed was unexpected and appears to be directed by the loss of Myb with significantly reduced tumor growth factor beta 1 (Tgfβ−1) expression by primary keratinocytes. Our findings support a specific role for Myb in K14+ epithelial cells in the preservation of adult skin integrity and function. 2015 Journal Article http://hdl.handle.net/20.500.11937/56640 10.3109/08977194.2015.1016222 JAI Press restricted |
| spellingShingle | Sampurno, S. Cross, R. Pearson, H. Kaur, Pritinder Malaterre, J. Ramsay, R. Myb via TGFβ is required for collagen type 1 production and skin integrity |
| title | Myb via TGFβ is required for collagen type 1 production and skin integrity |
| title_full | Myb via TGFβ is required for collagen type 1 production and skin integrity |
| title_fullStr | Myb via TGFβ is required for collagen type 1 production and skin integrity |
| title_full_unstemmed | Myb via TGFβ is required for collagen type 1 production and skin integrity |
| title_short | Myb via TGFβ is required for collagen type 1 production and skin integrity |
| title_sort | myb via tgfβ is required for collagen type 1 production and skin integrity |
| url | http://hdl.handle.net/20.500.11937/56640 |