Electrokinetic potential-stabilization by bile acid-microencapsulating formulation of pancreatic ß-cells cultured in high ratio poly-L-ornithine-gel hydrogel colloidal dispersion: applications in cell-biomaterials, tissue engineering and biotechnological applications
© 2017 Informa UK Limited, trading as Taylor & Francis Group Introduction: Current trials for ß-cell transplantation have been hindered by poor cell viability and function post-transplantation. Recently, electric charges of the microencapsulating formulation carrying ß-cells have shown signifi...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
Informa Healthcare
2017
|
| Online Access: | http://hdl.handle.net/20.500.11937/56344 |
| _version_ | 1848759851008655360 |
|---|---|
| author | Mooranian, A. Negrulj, R. Takechi, Ryu Jamieson, E. Morahan, G. Al-Salami, H. |
| author_facet | Mooranian, A. Negrulj, R. Takechi, Ryu Jamieson, E. Morahan, G. Al-Salami, H. |
| author_sort | Mooranian, A. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2017 Informa UK Limited, trading as Taylor & Francis Group Introduction: Current trials for ß-cell transplantation have been hindered by poor cell viability and function post-transplantation. Recently, electric charges of the microencapsulating formulation carrying ß-cells have shown significant effects on cell survival and function. Thus, this study aimed at investigating the effects of electric charge, of novel colloidal formulation containing ß-cells, on cell viability, biological activity and insulin release. Methods: A new formulation, containing high ratios of poly-L-ornithine, suspending electrical-stimulation hydrogel and polystyrene sulphone (1:1:0.1 ratio), was used to form microcapsules utilizing 800?V and 2000?Hz encapsulating conditions. The bile acid, ursodeoxycholic acid, was added into the microcapsules to measure its effects on electric charges. Results: The electric charge of the microencapsulating formulation was enhanced by bile acid addition, and resulted in better cell viability and function. Conclusion: Ursodeoxycholic acid microencapsulated with poly-L-ornithine, suspending electrical-stimulation hydrogel and polystyrene sulphone at 1:1:0.1 ratio, using 800?V and 2000?Hz microencapsulating conditions, produced enhanced electrokinetic parameters of microcapsules with optimized cell functions. This suggests that electric charge of formulations containing pancreatic ß-cell may have significant effects on cell mass and functions, post-transplantation. |
| first_indexed | 2025-11-14T10:06:27Z |
| format | Journal Article |
| id | curtin-20.500.11937-56344 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:06:27Z |
| publishDate | 2017 |
| publisher | Informa Healthcare |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-563442017-09-13T16:10:28Z Electrokinetic potential-stabilization by bile acid-microencapsulating formulation of pancreatic ß-cells cultured in high ratio poly-L-ornithine-gel hydrogel colloidal dispersion: applications in cell-biomaterials, tissue engineering and biotechnological applications Mooranian, A. Negrulj, R. Takechi, Ryu Jamieson, E. Morahan, G. Al-Salami, H. © 2017 Informa UK Limited, trading as Taylor & Francis Group Introduction: Current trials for ß-cell transplantation have been hindered by poor cell viability and function post-transplantation. Recently, electric charges of the microencapsulating formulation carrying ß-cells have shown significant effects on cell survival and function. Thus, this study aimed at investigating the effects of electric charge, of novel colloidal formulation containing ß-cells, on cell viability, biological activity and insulin release. Methods: A new formulation, containing high ratios of poly-L-ornithine, suspending electrical-stimulation hydrogel and polystyrene sulphone (1:1:0.1 ratio), was used to form microcapsules utilizing 800?V and 2000?Hz encapsulating conditions. The bile acid, ursodeoxycholic acid, was added into the microcapsules to measure its effects on electric charges. Results: The electric charge of the microencapsulating formulation was enhanced by bile acid addition, and resulted in better cell viability and function. Conclusion: Ursodeoxycholic acid microencapsulated with poly-L-ornithine, suspending electrical-stimulation hydrogel and polystyrene sulphone at 1:1:0.1 ratio, using 800?V and 2000?Hz microencapsulating conditions, produced enhanced electrokinetic parameters of microcapsules with optimized cell functions. This suggests that electric charge of formulations containing pancreatic ß-cell may have significant effects on cell mass and functions, post-transplantation. 2017 Journal Article http://hdl.handle.net/20.500.11937/56344 10.1080/21691401.2017.1362416 Informa Healthcare restricted |
| spellingShingle | Mooranian, A. Negrulj, R. Takechi, Ryu Jamieson, E. Morahan, G. Al-Salami, H. Electrokinetic potential-stabilization by bile acid-microencapsulating formulation of pancreatic ß-cells cultured in high ratio poly-L-ornithine-gel hydrogel colloidal dispersion: applications in cell-biomaterials, tissue engineering and biotechnological applications |
| title | Electrokinetic potential-stabilization by bile acid-microencapsulating formulation of pancreatic ß-cells cultured in high ratio poly-L-ornithine-gel hydrogel colloidal dispersion: applications in cell-biomaterials, tissue engineering and biotechnological applications |
| title_full | Electrokinetic potential-stabilization by bile acid-microencapsulating formulation of pancreatic ß-cells cultured in high ratio poly-L-ornithine-gel hydrogel colloidal dispersion: applications in cell-biomaterials, tissue engineering and biotechnological applications |
| title_fullStr | Electrokinetic potential-stabilization by bile acid-microencapsulating formulation of pancreatic ß-cells cultured in high ratio poly-L-ornithine-gel hydrogel colloidal dispersion: applications in cell-biomaterials, tissue engineering and biotechnological applications |
| title_full_unstemmed | Electrokinetic potential-stabilization by bile acid-microencapsulating formulation of pancreatic ß-cells cultured in high ratio poly-L-ornithine-gel hydrogel colloidal dispersion: applications in cell-biomaterials, tissue engineering and biotechnological applications |
| title_short | Electrokinetic potential-stabilization by bile acid-microencapsulating formulation of pancreatic ß-cells cultured in high ratio poly-L-ornithine-gel hydrogel colloidal dispersion: applications in cell-biomaterials, tissue engineering and biotechnological applications |
| title_sort | electrokinetic potential-stabilization by bile acid-microencapsulating formulation of pancreatic ß-cells cultured in high ratio poly-l-ornithine-gel hydrogel colloidal dispersion: applications in cell-biomaterials, tissue engineering and biotechnological applications |
| url | http://hdl.handle.net/20.500.11937/56344 |