Cytomegalovirus infection alters phenotypes of different ?d T-cell subsets in renal transplant recipients with long-term stable graft function
© 2017 Wiley Periodicals, Inc. Cytomegalovirus (CMV) infection alters the phenotypic profiles of T-cells and NK cells in healthy and immunocompromised individuals. Here, we examined the effects of CMV infection on the phenotype and functions of ?d T-cell subsets in renal transplant recipients (RTR)...
| Main Authors: | , , , |
|---|---|
| Format: | Journal Article |
| Published: |
WILEY-LISS
2017
|
| Online Access: | http://hdl.handle.net/20.500.11937/56314 |
| _version_ | 1848759842685059072 |
|---|---|
| author | Lee, S. Affandi, Jacquita Irish, A. Price, P. |
| author_facet | Lee, S. Affandi, Jacquita Irish, A. Price, P. |
| author_sort | Lee, S. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2017 Wiley Periodicals, Inc. Cytomegalovirus (CMV) infection alters the phenotypic profiles of T-cells and NK cells in healthy and immunocompromised individuals. Here, we examined the effects of CMV infection on the phenotype and functions of ?d T-cell subsets in renal transplant recipients (RTR) stable several years after transplantation (n = 80) and healthy controls (n = 72). Differentiation status, function, and expression of HLA-DR, CD57, and LIR-1 on Vd2 - and Vd2 + ?d T-cells were examined in peripheral blood cells using flow cytometry. Percentages of Vd2 - ?d T-cells were higher in RTR who are CMV-seropositive and correlated with CMV antibody levels. Proportions of Vd2 - ?d T-cells expressing HLA-DR, CD57, or LIR-1 were increased in CMV-seropositive RTR and healthy controls compared to their seronegative counterparts. Additionally, Vd2 - ?d T-cells were skewed towards a terminally differentiated phenotype and most expressed CD8 in individuals who were CMV-seropositive. Increased expression of LIR-1 on terminally differentiated Vd2 - ?d T-cells was associated with CMV seropositivity in RTR and controls. The presence of CMV DNA in 15 RTR was associated with higher frequencies of LIR-1+ Vd2 + ?d T-cells and increased percentages of terminally differentiated effector memory cells in both ?d T-cell subsets. Our study further characterises the effects of CMV and transplantation on ?d T-cell phenotypes. |
| first_indexed | 2025-11-14T10:06:19Z |
| format | Journal Article |
| id | curtin-20.500.11937-56314 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:06:19Z |
| publishDate | 2017 |
| publisher | WILEY-LISS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-563142017-09-13T16:10:51Z Cytomegalovirus infection alters phenotypes of different ?d T-cell subsets in renal transplant recipients with long-term stable graft function Lee, S. Affandi, Jacquita Irish, A. Price, P. © 2017 Wiley Periodicals, Inc. Cytomegalovirus (CMV) infection alters the phenotypic profiles of T-cells and NK cells in healthy and immunocompromised individuals. Here, we examined the effects of CMV infection on the phenotype and functions of ?d T-cell subsets in renal transplant recipients (RTR) stable several years after transplantation (n = 80) and healthy controls (n = 72). Differentiation status, function, and expression of HLA-DR, CD57, and LIR-1 on Vd2 - and Vd2 + ?d T-cells were examined in peripheral blood cells using flow cytometry. Percentages of Vd2 - ?d T-cells were higher in RTR who are CMV-seropositive and correlated with CMV antibody levels. Proportions of Vd2 - ?d T-cells expressing HLA-DR, CD57, or LIR-1 were increased in CMV-seropositive RTR and healthy controls compared to their seronegative counterparts. Additionally, Vd2 - ?d T-cells were skewed towards a terminally differentiated phenotype and most expressed CD8 in individuals who were CMV-seropositive. Increased expression of LIR-1 on terminally differentiated Vd2 - ?d T-cells was associated with CMV seropositivity in RTR and controls. The presence of CMV DNA in 15 RTR was associated with higher frequencies of LIR-1+ Vd2 + ?d T-cells and increased percentages of terminally differentiated effector memory cells in both ?d T-cell subsets. Our study further characterises the effects of CMV and transplantation on ?d T-cell phenotypes. 2017 Journal Article http://hdl.handle.net/20.500.11937/56314 10.1002/jmv.24784 WILEY-LISS restricted |
| spellingShingle | Lee, S. Affandi, Jacquita Irish, A. Price, P. Cytomegalovirus infection alters phenotypes of different ?d T-cell subsets in renal transplant recipients with long-term stable graft function |
| title | Cytomegalovirus infection alters phenotypes of different ?d T-cell subsets in renal transplant recipients with long-term stable graft function |
| title_full | Cytomegalovirus infection alters phenotypes of different ?d T-cell subsets in renal transplant recipients with long-term stable graft function |
| title_fullStr | Cytomegalovirus infection alters phenotypes of different ?d T-cell subsets in renal transplant recipients with long-term stable graft function |
| title_full_unstemmed | Cytomegalovirus infection alters phenotypes of different ?d T-cell subsets in renal transplant recipients with long-term stable graft function |
| title_short | Cytomegalovirus infection alters phenotypes of different ?d T-cell subsets in renal transplant recipients with long-term stable graft function |
| title_sort | cytomegalovirus infection alters phenotypes of different ?d t-cell subsets in renal transplant recipients with long-term stable graft function |
| url | http://hdl.handle.net/20.500.11937/56314 |