Biocompatibility and cellular uptake mechanisms of poly(N -isopropylacrylamide) in different cells
© SAGE Publications. Thermosensitive poly(N-isopropylacrylamide) is widely used in various biomedical applications including drug delivery systems, gene delivery systems, switching devices, sensors, and diagnostic assays. To promote these clinical applications, it is essential to have a comprehensiv...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11937/55865 |
| _version_ | 1848759727303950336 |
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| author | Guo, Z. Li, S. Wang, C. Xu, J. Kirk, B. Wu, Jian-Ping Liu, Z. Xue, W. |
| author_facet | Guo, Z. Li, S. Wang, C. Xu, J. Kirk, B. Wu, Jian-Ping Liu, Z. Xue, W. |
| author_sort | Guo, Z. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © SAGE Publications. Thermosensitive poly(N-isopropylacrylamide) is widely used in various biomedical applications including drug delivery systems, gene delivery systems, switching devices, sensors, and diagnostic assays. To promote these clinical applications, it is essential to have a comprehensive understanding of the biosafety of poly(N-isopropylacrylamide) and the interaction of poly(N-isopropylacrylamide) with different cell lines, which has little research until now. In this work, we evaluated the biocompatibility of poly(N-isopropylacrylamide) including cell viability, nitric oxide production, and apoptosis of macrophages RAW264.7, human bronchial epithelial cells, A549, and human umbilical vein endothelial cells in the presence of poly(N-isopropylacrylamide). We have also examined the cellular uptake mechanisms of poly(N-isopropylacrylamide) using endocytic inhibitors and insighted into the intracellular co-localization of poly(N-isopropylacrylamide) using confocal laser scanning microscope. The results showed that poly(N-isopropylacrylamide) had good biocompatibility and could be internalized by these cells. It is macropinocytosis that poly(N-isopropylacrylamide) could be internalized in RAW264.7 cells and caveolae-mediated endocytosis in human bronchi al epithelial cells, A549, and human umbilical vein endothelial cells. In addition, we also evidenced that intracellular poly(N-isopropylacrylamide) was co-localized with lysosome. The study provided important information for the development and clinical applications of poly(N-isopropylacrylamide) in the biomedical field. |
| first_indexed | 2025-11-14T10:04:29Z |
| format | Journal Article |
| id | curtin-20.500.11937-55865 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:04:29Z |
| publishDate | 2017 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-558652017-09-13T16:10:51Z Biocompatibility and cellular uptake mechanisms of poly(N -isopropylacrylamide) in different cells Guo, Z. Li, S. Wang, C. Xu, J. Kirk, B. Wu, Jian-Ping Liu, Z. Xue, W. © SAGE Publications. Thermosensitive poly(N-isopropylacrylamide) is widely used in various biomedical applications including drug delivery systems, gene delivery systems, switching devices, sensors, and diagnostic assays. To promote these clinical applications, it is essential to have a comprehensive understanding of the biosafety of poly(N-isopropylacrylamide) and the interaction of poly(N-isopropylacrylamide) with different cell lines, which has little research until now. In this work, we evaluated the biocompatibility of poly(N-isopropylacrylamide) including cell viability, nitric oxide production, and apoptosis of macrophages RAW264.7, human bronchial epithelial cells, A549, and human umbilical vein endothelial cells in the presence of poly(N-isopropylacrylamide). We have also examined the cellular uptake mechanisms of poly(N-isopropylacrylamide) using endocytic inhibitors and insighted into the intracellular co-localization of poly(N-isopropylacrylamide) using confocal laser scanning microscope. The results showed that poly(N-isopropylacrylamide) had good biocompatibility and could be internalized by these cells. It is macropinocytosis that poly(N-isopropylacrylamide) could be internalized in RAW264.7 cells and caveolae-mediated endocytosis in human bronchi al epithelial cells, A549, and human umbilical vein endothelial cells. In addition, we also evidenced that intracellular poly(N-isopropylacrylamide) was co-localized with lysosome. The study provided important information for the development and clinical applications of poly(N-isopropylacrylamide) in the biomedical field. 2017 Journal Article http://hdl.handle.net/20.500.11937/55865 10.1177/0883911516648969 restricted |
| spellingShingle | Guo, Z. Li, S. Wang, C. Xu, J. Kirk, B. Wu, Jian-Ping Liu, Z. Xue, W. Biocompatibility and cellular uptake mechanisms of poly(N -isopropylacrylamide) in different cells |
| title | Biocompatibility and cellular uptake mechanisms of poly(N -isopropylacrylamide) in different cells |
| title_full | Biocompatibility and cellular uptake mechanisms of poly(N -isopropylacrylamide) in different cells |
| title_fullStr | Biocompatibility and cellular uptake mechanisms of poly(N -isopropylacrylamide) in different cells |
| title_full_unstemmed | Biocompatibility and cellular uptake mechanisms of poly(N -isopropylacrylamide) in different cells |
| title_short | Biocompatibility and cellular uptake mechanisms of poly(N -isopropylacrylamide) in different cells |
| title_sort | biocompatibility and cellular uptake mechanisms of poly(n -isopropylacrylamide) in different cells |
| url | http://hdl.handle.net/20.500.11937/55865 |