Cryo-mesh: a simple alternative cryopreservation protocol
BACKGROUND: The continued development of new cryopreservation protocols has improved post-cryogenic success rates for a wide variety of plant species. Methods like the cryo-plate have proven beneficial in simplifying the cryopreservation procedure. OBJECTIVE: This study assessed the practicality of...
| Main Authors: | , , |
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| Format: | Journal Article |
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CryoLetters
2017
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| Online Access: | http://hdl.handle.net/20.500.11937/55756 |
| _version_ | 1848759698863423488 |
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| author | Funnekotter, Bryn Bunn, E. Mancera, Ricardo |
| author_facet | Funnekotter, Bryn Bunn, E. Mancera, Ricardo |
| author_sort | Funnekotter, Bryn |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | BACKGROUND: The continued development of new cryopreservation protocols has improved post-cryogenic success rates for a wide variety of plant species. Methods like the cryo-plate have proven beneficial in simplifying the cryopreservation procedure. OBJECTIVE: This study assessed the practicality of a stainless steel mesh strip (cryo-mesh) for cryopreserving shoot tips from Anigozanthos viridis. MATERIALS AND METHODS: Shoot tips of A. viridis (Kangaroo Paw) were precultured on 0.4 M sucrose medium for 48 h. Precultured shoot tips were coated in a 2% alginate solution and placed onto the cryo-mesh (a 25 x 7 mm, 0.4 mm aperture, 0.224 mm diameter wire stainless steel mesh strip). The alginate was set for 20 min in a loading solution containing 100 mM CaCl2, anchoring the shoot tips to the cryo-mesh. The cryo-mesh was then transferred to PVS2 on ice for 20, 30 or 40 min prior to plunging the cryo-mesh into liquid nitrogen. The cryo-mesh protocol was compared to the droplet-vitrification protocol. RESULTS: A maximum of 83% post-cryogenic regeneration was achieved with the cryo-mesh when exposed to PVS2 for 30 min. No significant difference in post-cryogenic regeneration was observed between the cryo-mesh and droplet-vitrification protocols. CONCLUSION: Anigozanthos viridis shoot tips were successfully cryopreserved utilising the new cryo-mesh. The cryo-mesh thus provides a simple and successful alternative for cryopreservation. |
| first_indexed | 2025-11-14T10:04:01Z |
| format | Journal Article |
| id | curtin-20.500.11937-55756 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:04:01Z |
| publishDate | 2017 |
| publisher | CryoLetters |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-557562017-08-24T02:20:21Z Cryo-mesh: a simple alternative cryopreservation protocol Funnekotter, Bryn Bunn, E. Mancera, Ricardo BACKGROUND: The continued development of new cryopreservation protocols has improved post-cryogenic success rates for a wide variety of plant species. Methods like the cryo-plate have proven beneficial in simplifying the cryopreservation procedure. OBJECTIVE: This study assessed the practicality of a stainless steel mesh strip (cryo-mesh) for cryopreserving shoot tips from Anigozanthos viridis. MATERIALS AND METHODS: Shoot tips of A. viridis (Kangaroo Paw) were precultured on 0.4 M sucrose medium for 48 h. Precultured shoot tips were coated in a 2% alginate solution and placed onto the cryo-mesh (a 25 x 7 mm, 0.4 mm aperture, 0.224 mm diameter wire stainless steel mesh strip). The alginate was set for 20 min in a loading solution containing 100 mM CaCl2, anchoring the shoot tips to the cryo-mesh. The cryo-mesh was then transferred to PVS2 on ice for 20, 30 or 40 min prior to plunging the cryo-mesh into liquid nitrogen. The cryo-mesh protocol was compared to the droplet-vitrification protocol. RESULTS: A maximum of 83% post-cryogenic regeneration was achieved with the cryo-mesh when exposed to PVS2 for 30 min. No significant difference in post-cryogenic regeneration was observed between the cryo-mesh and droplet-vitrification protocols. CONCLUSION: Anigozanthos viridis shoot tips were successfully cryopreserved utilising the new cryo-mesh. The cryo-mesh thus provides a simple and successful alternative for cryopreservation. 2017 Journal Article http://hdl.handle.net/20.500.11937/55756 CryoLetters restricted |
| spellingShingle | Funnekotter, Bryn Bunn, E. Mancera, Ricardo Cryo-mesh: a simple alternative cryopreservation protocol |
| title | Cryo-mesh: a simple alternative cryopreservation protocol |
| title_full | Cryo-mesh: a simple alternative cryopreservation protocol |
| title_fullStr | Cryo-mesh: a simple alternative cryopreservation protocol |
| title_full_unstemmed | Cryo-mesh: a simple alternative cryopreservation protocol |
| title_short | Cryo-mesh: a simple alternative cryopreservation protocol |
| title_sort | cryo-mesh: a simple alternative cryopreservation protocol |
| url | http://hdl.handle.net/20.500.11937/55756 |