A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery
© 2016 Elsevier B.V. It is a promising way to treat the multi drug resistance (MDR) of tumor cells in both of drug and gene methods. A polyamidoamne dendrimer functionalized graphene oxide (GO-PAMAM) was designed, which could load doxorubicin (DOX) and MMP-9 shRNA plasmid at the same time in order t...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
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Elsevier
2017
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| Online Access: | http://hdl.handle.net/20.500.11937/55221 |
| _version_ | 1848759566267842560 |
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| author | Gu, Y. Guo, Y. Wang, C. Xu, J. Wu, Jian-Ping Kirk, T. Ma, D. Xue, W. |
| author_facet | Gu, Y. Guo, Y. Wang, C. Xu, J. Wu, Jian-Ping Kirk, T. Ma, D. Xue, W. |
| author_sort | Gu, Y. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2016 Elsevier B.V. It is a promising way to treat the multi drug resistance (MDR) of tumor cells in both of drug and gene methods. A polyamidoamne dendrimer functionalized graphene oxide (GO-PAMAM) was designed, which could load doxorubicin (DOX) and MMP-9 shRNA plasmid at the same time in order to achieve effective treatment to breast cancer. GO-PAMAM has a high loading capacity to DOX and pH-controlled DOX release. Besides, it has efficient gene transfer ability, the transfection efficiency is significantly better than PEI-25k in the presence of serum, and it can significantly inhibit the expression of MMP-9 protein in MCF-7 cells. The effect of DOX and MMP-9 shRNA plasmid co-delivery was more significant than that of the single drug. Moreover, GO-PAMAM exhibited lower cytotoxicity compared to PEI-25k in CCK-8 assays, and also showed a good biocompatibility in vivo. Therefore, GO-PAMAM will have broad prospects for drug and gene co-delivery. |
| first_indexed | 2025-11-14T10:01:55Z |
| format | Journal Article |
| id | curtin-20.500.11937-55221 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:01:55Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-552212017-09-13T16:11:12Z A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery Gu, Y. Guo, Y. Wang, C. Xu, J. Wu, Jian-Ping Kirk, T. Ma, D. Xue, W. © 2016 Elsevier B.V. It is a promising way to treat the multi drug resistance (MDR) of tumor cells in both of drug and gene methods. A polyamidoamne dendrimer functionalized graphene oxide (GO-PAMAM) was designed, which could load doxorubicin (DOX) and MMP-9 shRNA plasmid at the same time in order to achieve effective treatment to breast cancer. GO-PAMAM has a high loading capacity to DOX and pH-controlled DOX release. Besides, it has efficient gene transfer ability, the transfection efficiency is significantly better than PEI-25k in the presence of serum, and it can significantly inhibit the expression of MMP-9 protein in MCF-7 cells. The effect of DOX and MMP-9 shRNA plasmid co-delivery was more significant than that of the single drug. Moreover, GO-PAMAM exhibited lower cytotoxicity compared to PEI-25k in CCK-8 assays, and also showed a good biocompatibility in vivo. Therefore, GO-PAMAM will have broad prospects for drug and gene co-delivery. 2017 Journal Article http://hdl.handle.net/20.500.11937/55221 10.1016/j.msec.2016.09.035 Elsevier restricted |
| spellingShingle | Gu, Y. Guo, Y. Wang, C. Xu, J. Wu, Jian-Ping Kirk, T. Ma, D. Xue, W. A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery |
| title | A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery |
| title_full | A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery |
| title_fullStr | A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery |
| title_full_unstemmed | A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery |
| title_short | A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery |
| title_sort | polyamidoamne dendrimer functionalized graphene oxide for dox and mmp-9 shrna plasmid co-delivery |
| url | http://hdl.handle.net/20.500.11937/55221 |