Characterization, development and multiplexing of microsatellite markers in three commercially exploited reef fish and their application for stock identification
© 2016 Taillebois et al.Thirty-four microsatellite loci were isolated from three reef fish species; golden snapper Lutjanus johnii, blackspotted croaker Protonibea diacanthus and grass emperor Lethrinus laticaudis using a next generation sequencing approach. Both IonTorrent single reads and Illumina...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
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PeerJ, Ltd.
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/52360 |
| _version_ | 1848758909254238208 |
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| author | Taillebois, L. Dudgeon, C. Maher, S. Crook, D. Saunders, T. Barton, D. Taylor, J. Welch, D. Newman, Stephen Travers, M. Saunders, R. Ovenden, J. |
| author_facet | Taillebois, L. Dudgeon, C. Maher, S. Crook, D. Saunders, T. Barton, D. Taylor, J. Welch, D. Newman, Stephen Travers, M. Saunders, R. Ovenden, J. |
| author_sort | Taillebois, L. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2016 Taillebois et al.Thirty-four microsatellite loci were isolated from three reef fish species; golden snapper Lutjanus johnii, blackspotted croaker Protonibea diacanthus and grass emperor Lethrinus laticaudis using a next generation sequencing approach. Both IonTorrent single reads and Illumina MiSeq paired-end reads were used, with the latter demonstrating a higher quality of reads than the IonTorrent. From the 1-1.5 million raw reads per species, we successfully obtained 10-13 polymorphic loci for each species, which satisfied stringent design criteria. We developed multiplex panels for the amplification of the golden snapper and the blackspotted croaker loci, as well as post-amplification pooling panels for the grass emperor loci. The microsatellites characterized in this work were tested across three locations of northern Australia. The microsatellites we developed can detect population differentiation across northern Australia and may be used for genetic structure studies and stock identification. |
| first_indexed | 2025-11-14T09:51:28Z |
| format | Journal Article |
| id | curtin-20.500.11937-52360 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:51:28Z |
| publishDate | 2016 |
| publisher | PeerJ, Ltd. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-523602017-09-13T15:39:04Z Characterization, development and multiplexing of microsatellite markers in three commercially exploited reef fish and their application for stock identification Taillebois, L. Dudgeon, C. Maher, S. Crook, D. Saunders, T. Barton, D. Taylor, J. Welch, D. Newman, Stephen Travers, M. Saunders, R. Ovenden, J. © 2016 Taillebois et al.Thirty-four microsatellite loci were isolated from three reef fish species; golden snapper Lutjanus johnii, blackspotted croaker Protonibea diacanthus and grass emperor Lethrinus laticaudis using a next generation sequencing approach. Both IonTorrent single reads and Illumina MiSeq paired-end reads were used, with the latter demonstrating a higher quality of reads than the IonTorrent. From the 1-1.5 million raw reads per species, we successfully obtained 10-13 polymorphic loci for each species, which satisfied stringent design criteria. We developed multiplex panels for the amplification of the golden snapper and the blackspotted croaker loci, as well as post-amplification pooling panels for the grass emperor loci. The microsatellites characterized in this work were tested across three locations of northern Australia. The microsatellites we developed can detect population differentiation across northern Australia and may be used for genetic structure studies and stock identification. 2016 Journal Article http://hdl.handle.net/20.500.11937/52360 10.7717/PEERJ.2418 PeerJ, Ltd. unknown |
| spellingShingle | Taillebois, L. Dudgeon, C. Maher, S. Crook, D. Saunders, T. Barton, D. Taylor, J. Welch, D. Newman, Stephen Travers, M. Saunders, R. Ovenden, J. Characterization, development and multiplexing of microsatellite markers in three commercially exploited reef fish and their application for stock identification |
| title | Characterization, development and multiplexing of microsatellite markers in three commercially exploited reef fish and their application for stock identification |
| title_full | Characterization, development and multiplexing of microsatellite markers in three commercially exploited reef fish and their application for stock identification |
| title_fullStr | Characterization, development and multiplexing of microsatellite markers in three commercially exploited reef fish and their application for stock identification |
| title_full_unstemmed | Characterization, development and multiplexing of microsatellite markers in three commercially exploited reef fish and their application for stock identification |
| title_short | Characterization, development and multiplexing of microsatellite markers in three commercially exploited reef fish and their application for stock identification |
| title_sort | characterization, development and multiplexing of microsatellite markers in three commercially exploited reef fish and their application for stock identification |
| url | http://hdl.handle.net/20.500.11937/52360 |