Osteopontin Stabilizes Metastable States Prior to Nucleation during Apatite Formation
© 2016 American Chemical Society.Osteopontin, which is a phosphoprotein with strong ties to in vivo bone mineralization, is shown to change the precipitation pathway of calcium phosphate. We show that the presence of the phosphoprotein, even in minute concentrations, can stabilize an otherwise overs...
| Main Authors: | , , |
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| Format: | Journal Article |
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American Chemical Society
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/52203 |
| _version_ | 1848758871139549184 |
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| author | Ibsen, C. Gebauer, Denis Birkedal, H. |
| author_facet | Ibsen, C. Gebauer, Denis Birkedal, H. |
| author_sort | Ibsen, C. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2016 American Chemical Society.Osteopontin, which is a phosphoprotein with strong ties to in vivo bone mineralization, is shown to change the precipitation pathway of calcium phosphate. We show that the presence of the phosphoprotein, even in minute concentrations, can stabilize an otherwise oversaturated mixture against precipitation. At moderate concentrations, we find that the protein introduces a new intermediate state into the reaction pathway leading to apatite formation. This new intermediate was found to share many characteristics of a coacervate or polymer-induced liquid-like precursor (PILP) phase. Our results show that these types of complex phases should be considered when discussing the mechanisms of bone mineralization on a subcellular level. |
| first_indexed | 2025-11-14T09:50:52Z |
| format | Journal Article |
| id | curtin-20.500.11937-52203 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:50:52Z |
| publishDate | 2016 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-522032017-09-13T15:39:23Z Osteopontin Stabilizes Metastable States Prior to Nucleation during Apatite Formation Ibsen, C. Gebauer, Denis Birkedal, H. © 2016 American Chemical Society.Osteopontin, which is a phosphoprotein with strong ties to in vivo bone mineralization, is shown to change the precipitation pathway of calcium phosphate. We show that the presence of the phosphoprotein, even in minute concentrations, can stabilize an otherwise oversaturated mixture against precipitation. At moderate concentrations, we find that the protein introduces a new intermediate state into the reaction pathway leading to apatite formation. This new intermediate was found to share many characteristics of a coacervate or polymer-induced liquid-like precursor (PILP) phase. Our results show that these types of complex phases should be considered when discussing the mechanisms of bone mineralization on a subcellular level. 2016 Journal Article http://hdl.handle.net/20.500.11937/52203 10.1021/acs.chemmater.6b01088 American Chemical Society restricted |
| spellingShingle | Ibsen, C. Gebauer, Denis Birkedal, H. Osteopontin Stabilizes Metastable States Prior to Nucleation during Apatite Formation |
| title | Osteopontin Stabilizes Metastable States Prior to Nucleation during Apatite Formation |
| title_full | Osteopontin Stabilizes Metastable States Prior to Nucleation during Apatite Formation |
| title_fullStr | Osteopontin Stabilizes Metastable States Prior to Nucleation during Apatite Formation |
| title_full_unstemmed | Osteopontin Stabilizes Metastable States Prior to Nucleation during Apatite Formation |
| title_short | Osteopontin Stabilizes Metastable States Prior to Nucleation during Apatite Formation |
| title_sort | osteopontin stabilizes metastable states prior to nucleation during apatite formation |
| url | http://hdl.handle.net/20.500.11937/52203 |