Functional genomics-guided discovery of a light-activated phytotoxin in the wheat pathogen Parastagonospora nodorum via pathway activation
© 2017 Society for Applied Microbiology and John Wiley & Sons Ltd.Parastagonospora nodorum is an important pathogen of wheat. The contribution of secondary metabolites to this pathosystem is poorly understood. A biosynthetic gene cluster (SNOG_08608-08616) has been shown to be upregulated during...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
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Wiley-Blackwell Publishing
2017
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| Online Access: | http://hdl.handle.net/20.500.11937/52118 |
| _version_ | 1848758851078193152 |
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| author | Chooi, Y. Zhang, G. Hu, J. Muria Gonzalez, Jordi Tran, P. Pettitt, A. Maier, A. Barrow, R. Solomon, P. |
| author_facet | Chooi, Y. Zhang, G. Hu, J. Muria Gonzalez, Jordi Tran, P. Pettitt, A. Maier, A. Barrow, R. Solomon, P. |
| author_sort | Chooi, Y. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2017 Society for Applied Microbiology and John Wiley & Sons Ltd.Parastagonospora nodorum is an important pathogen of wheat. The contribution of secondary metabolites to this pathosystem is poorly understood. A biosynthetic gene cluster (SNOG_08608-08616) has been shown to be upregulated during the late stage of P. nodorum wheat leaf infection. The gene cluster shares several homologues with the Cercospora nicotianae CTB gene cluster encoding the biosynthesis of cercosporin. Activation of the gene cluster by overexpression (OE) of the transcription factor gene (SNOG_08609) in P. nodorum resulted in the production of elsinochrome C, a perelyenequinone phytotoxin structurally similar to cercosporin. Heterologous expression of the polyketide synthase gene elcA from the gene cluster in Aspergillus nidulans resulted in the production of the polyketide precursor nortoralactone common to the cercosporin pathway. Elsinochrome C could be detected on wheat leaves infected with P. nodorum, but not in the elcA disruption mutant. The compound was shown to exhibit necrotic activity on wheat leaves in a light-dependent manner. Wheat seedling infection assays showed that ?elcA exhibited reduced virulence compared with wild type, while infection by an OE strain overproducing elsinochrome C resulted in larger lesions on leaves. These data provided evidence that elsinochrome C contributes to the virulence of P. nodorum against wheat. |
| first_indexed | 2025-11-14T09:50:33Z |
| format | Journal Article |
| id | curtin-20.500.11937-52118 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:50:33Z |
| publishDate | 2017 |
| publisher | Wiley-Blackwell Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-521182017-09-13T15:40:24Z Functional genomics-guided discovery of a light-activated phytotoxin in the wheat pathogen Parastagonospora nodorum via pathway activation Chooi, Y. Zhang, G. Hu, J. Muria Gonzalez, Jordi Tran, P. Pettitt, A. Maier, A. Barrow, R. Solomon, P. © 2017 Society for Applied Microbiology and John Wiley & Sons Ltd.Parastagonospora nodorum is an important pathogen of wheat. The contribution of secondary metabolites to this pathosystem is poorly understood. A biosynthetic gene cluster (SNOG_08608-08616) has been shown to be upregulated during the late stage of P. nodorum wheat leaf infection. The gene cluster shares several homologues with the Cercospora nicotianae CTB gene cluster encoding the biosynthesis of cercosporin. Activation of the gene cluster by overexpression (OE) of the transcription factor gene (SNOG_08609) in P. nodorum resulted in the production of elsinochrome C, a perelyenequinone phytotoxin structurally similar to cercosporin. Heterologous expression of the polyketide synthase gene elcA from the gene cluster in Aspergillus nidulans resulted in the production of the polyketide precursor nortoralactone common to the cercosporin pathway. Elsinochrome C could be detected on wheat leaves infected with P. nodorum, but not in the elcA disruption mutant. The compound was shown to exhibit necrotic activity on wheat leaves in a light-dependent manner. Wheat seedling infection assays showed that ?elcA exhibited reduced virulence compared with wild type, while infection by an OE strain overproducing elsinochrome C resulted in larger lesions on leaves. These data provided evidence that elsinochrome C contributes to the virulence of P. nodorum against wheat. 2017 Journal Article http://hdl.handle.net/20.500.11937/52118 10.1111/1462-2920.13711 Wiley-Blackwell Publishing restricted |
| spellingShingle | Chooi, Y. Zhang, G. Hu, J. Muria Gonzalez, Jordi Tran, P. Pettitt, A. Maier, A. Barrow, R. Solomon, P. Functional genomics-guided discovery of a light-activated phytotoxin in the wheat pathogen Parastagonospora nodorum via pathway activation |
| title | Functional genomics-guided discovery of a light-activated phytotoxin in the wheat pathogen Parastagonospora nodorum via pathway activation |
| title_full | Functional genomics-guided discovery of a light-activated phytotoxin in the wheat pathogen Parastagonospora nodorum via pathway activation |
| title_fullStr | Functional genomics-guided discovery of a light-activated phytotoxin in the wheat pathogen Parastagonospora nodorum via pathway activation |
| title_full_unstemmed | Functional genomics-guided discovery of a light-activated phytotoxin in the wheat pathogen Parastagonospora nodorum via pathway activation |
| title_short | Functional genomics-guided discovery of a light-activated phytotoxin in the wheat pathogen Parastagonospora nodorum via pathway activation |
| title_sort | functional genomics-guided discovery of a light-activated phytotoxin in the wheat pathogen parastagonospora nodorum via pathway activation |
| url | http://hdl.handle.net/20.500.11937/52118 |