HLA typing by sequence-specific primers
HLA typing by sequence-specific primers (PCR-SSP) is a commonly used technique in HLA typing in which multiple pairs of cis-located allele-specific primers are used to determine the alleles present in a given DNA sample. Although the technique is around two decades old, it still offers a relatively...
| Main Authors: | , |
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| Format: | Book Chapter |
| Published: |
Humana Press
2013
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| Online Access: | http://hdl.handle.net/20.500.11937/51990 |
| _version_ | 1848758815879593984 |
|---|---|
| author | Bunce, Michael Passey, B. |
| author_facet | Bunce, Michael Passey, B. |
| author_sort | Bunce, Michael |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | HLA typing by sequence-specific primers (PCR-SSP) is a commonly used technique in HLA typing in which multiple pairs of cis-located allele-specific primers are used to determine the alleles present in a given DNA sample. Although the technique is around two decades old, it still offers a relatively straight forward way of typing and has the benefit of using commonly available laboratory equipment. Here we describe the background of the PCR-SSP, how to design and validate reactions, and common problems that arise. © Springer Science+Business Media, LLC 2013. |
| first_indexed | 2025-11-14T09:49:59Z |
| format | Book Chapter |
| id | curtin-20.500.11937-51990 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:49:59Z |
| publishDate | 2013 |
| publisher | Humana Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-519902017-09-13T15:48:31Z HLA typing by sequence-specific primers Bunce, Michael Passey, B. HLA typing by sequence-specific primers (PCR-SSP) is a commonly used technique in HLA typing in which multiple pairs of cis-located allele-specific primers are used to determine the alleles present in a given DNA sample. Although the technique is around two decades old, it still offers a relatively straight forward way of typing and has the benefit of using commonly available laboratory equipment. Here we describe the background of the PCR-SSP, how to design and validate reactions, and common problems that arise. © Springer Science+Business Media, LLC 2013. 2013 Book Chapter http://hdl.handle.net/20.500.11937/51990 10.1007/978-1-62703-493-7_8 Humana Press restricted |
| spellingShingle | Bunce, Michael Passey, B. HLA typing by sequence-specific primers |
| title | HLA typing by sequence-specific primers |
| title_full | HLA typing by sequence-specific primers |
| title_fullStr | HLA typing by sequence-specific primers |
| title_full_unstemmed | HLA typing by sequence-specific primers |
| title_short | HLA typing by sequence-specific primers |
| title_sort | hla typing by sequence-specific primers |
| url | http://hdl.handle.net/20.500.11937/51990 |