DEAD-box helicase DP103 defines metastatic potential of human breast cancers
Despite advancement in breast cancer treatment, 30% of patients with early breast cancers experience relapse with distant metastasis. It is a challenge to identify patients at risk for relapse; therefore, the identification of markers and therapeutic targets for metastatic breast cancers is imperati...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Published: |
American Society for Clinical Investigation
2014
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| Online Access: | http://hdl.handle.net/20.500.11937/51066 |
| _version_ | 1848758605923221504 |
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| author | Shin, E. Hay, H. Lee, M. Goh, J. Tan, T. Sen, Y. Lim, S. Yousef, E. Ong, H. Thike, A. Kong, X. Wu, Z. Mendoz, E. Sun, W. Salto-Tellez, M. Lim, C. Lobie, P. Lim, Y. Yap, C. Zeng, Q. Sethi, G. Lee, M. Tan, P. Goh, B. Miller, L. Thiery, J. Zhu, T. Gaboury, L. Tan, P. Hui, K. Yip, G. Miyamoto, S. Kumar, Alan Prem Tergaonkar, V. |
| author_facet | Shin, E. Hay, H. Lee, M. Goh, J. Tan, T. Sen, Y. Lim, S. Yousef, E. Ong, H. Thike, A. Kong, X. Wu, Z. Mendoz, E. Sun, W. Salto-Tellez, M. Lim, C. Lobie, P. Lim, Y. Yap, C. Zeng, Q. Sethi, G. Lee, M. Tan, P. Goh, B. Miller, L. Thiery, J. Zhu, T. Gaboury, L. Tan, P. Hui, K. Yip, G. Miyamoto, S. Kumar, Alan Prem Tergaonkar, V. |
| author_sort | Shin, E. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Despite advancement in breast cancer treatment, 30% of patients with early breast cancers experience relapse with distant metastasis. It is a challenge to identify patients at risk for relapse; therefore, the identification of markers and therapeutic targets for metastatic breast cancers is imperative. Here, we identified DP103 as a biomarker and metastasis-driving oncogene in human breast cancers and determined that DP103 elevates matrix metallopeptidase 9 (MMP9) levels, which are associated with metastasis and invasion through activation of NF-κB. In turn, NF-κB signaling positively activated DP103 expression. Furthermore, DP103 enhanced TGF-β–activated kinase-1 (TAK1) phosphorylation of NF-κB–activating IκB kinase 2 (IKK2), leading to increased NF-κB activity. Reduction of DP103 expression in invasive breast cancer cells reduced phosphorylation of IKK2, abrogated NF-κB–mediated MMP9 expression, and impeded metastasis in a murine xenograft model. In breast cancer patient tissues, elevated levels of DP103 correlated with enhanced MMP9, reduced overall survival, and reduced survival after relapse. Together, these data indicate that a positive DP103/NF-κB feedback loop promotes constitutive NF-κB activation in invasive breast cancers and activation of this pathway is linked to cancer progression and the acquisition of chemotherapy resistance. Furthermore, our results suggest that DP103 has potential as a therapeutic target for breast cancer treatment. |
| first_indexed | 2025-11-14T09:46:39Z |
| format | Journal Article |
| id | curtin-20.500.11937-51066 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:46:39Z |
| publishDate | 2014 |
| publisher | American Society for Clinical Investigation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-510662017-09-13T15:49:25Z DEAD-box helicase DP103 defines metastatic potential of human breast cancers Shin, E. Hay, H. Lee, M. Goh, J. Tan, T. Sen, Y. Lim, S. Yousef, E. Ong, H. Thike, A. Kong, X. Wu, Z. Mendoz, E. Sun, W. Salto-Tellez, M. Lim, C. Lobie, P. Lim, Y. Yap, C. Zeng, Q. Sethi, G. Lee, M. Tan, P. Goh, B. Miller, L. Thiery, J. Zhu, T. Gaboury, L. Tan, P. Hui, K. Yip, G. Miyamoto, S. Kumar, Alan Prem Tergaonkar, V. Despite advancement in breast cancer treatment, 30% of patients with early breast cancers experience relapse with distant metastasis. It is a challenge to identify patients at risk for relapse; therefore, the identification of markers and therapeutic targets for metastatic breast cancers is imperative. Here, we identified DP103 as a biomarker and metastasis-driving oncogene in human breast cancers and determined that DP103 elevates matrix metallopeptidase 9 (MMP9) levels, which are associated with metastasis and invasion through activation of NF-κB. In turn, NF-κB signaling positively activated DP103 expression. Furthermore, DP103 enhanced TGF-β–activated kinase-1 (TAK1) phosphorylation of NF-κB–activating IκB kinase 2 (IKK2), leading to increased NF-κB activity. Reduction of DP103 expression in invasive breast cancer cells reduced phosphorylation of IKK2, abrogated NF-κB–mediated MMP9 expression, and impeded metastasis in a murine xenograft model. In breast cancer patient tissues, elevated levels of DP103 correlated with enhanced MMP9, reduced overall survival, and reduced survival after relapse. Together, these data indicate that a positive DP103/NF-κB feedback loop promotes constitutive NF-κB activation in invasive breast cancers and activation of this pathway is linked to cancer progression and the acquisition of chemotherapy resistance. Furthermore, our results suggest that DP103 has potential as a therapeutic target for breast cancer treatment. 2014 Journal Article http://hdl.handle.net/20.500.11937/51066 10.1172/JCI73451 American Society for Clinical Investigation unknown |
| spellingShingle | Shin, E. Hay, H. Lee, M. Goh, J. Tan, T. Sen, Y. Lim, S. Yousef, E. Ong, H. Thike, A. Kong, X. Wu, Z. Mendoz, E. Sun, W. Salto-Tellez, M. Lim, C. Lobie, P. Lim, Y. Yap, C. Zeng, Q. Sethi, G. Lee, M. Tan, P. Goh, B. Miller, L. Thiery, J. Zhu, T. Gaboury, L. Tan, P. Hui, K. Yip, G. Miyamoto, S. Kumar, Alan Prem Tergaonkar, V. DEAD-box helicase DP103 defines metastatic potential of human breast cancers |
| title | DEAD-box helicase DP103 defines metastatic potential of human breast cancers |
| title_full | DEAD-box helicase DP103 defines metastatic potential of human breast cancers |
| title_fullStr | DEAD-box helicase DP103 defines metastatic potential of human breast cancers |
| title_full_unstemmed | DEAD-box helicase DP103 defines metastatic potential of human breast cancers |
| title_short | DEAD-box helicase DP103 defines metastatic potential of human breast cancers |
| title_sort | dead-box helicase dp103 defines metastatic potential of human breast cancers |
| url | http://hdl.handle.net/20.500.11937/51066 |