Cardamonin represses proliferation, invasion, and causes apoptosis through the modulation of signal transducer and activator of transcription 3 pathway in prostate cancer

The pleiotropic transcription factor, signal transducer and activator of transcription 3 (STAT3) is often aberrantly activated in a wide variety of cancers and plays a pivotal role in tumor initiation, promotion and progression. Targeting deregulated STAT3 activation by small molecule inhibitors is...

Full description

Bibliographic Details
Main Authors: Zhang, J., Sikka, S., Siveen, K., Lee, J., Um, J., Kumar, Alan Prem, Chinnathambi, A., Alharbi, S., Basappa, Rangappa, K., Sethi, G., Ahn, K.
Format: Journal Article
Published: Springer 2017
Online Access:http://hdl.handle.net/20.500.11937/50872
_version_ 1848758556624420864
author Zhang, J.
Sikka, S.
Siveen, K.
Lee, J.
Um, J.
Kumar, Alan Prem
Chinnathambi, A.
Alharbi, S.
Basappa
Rangappa, K.
Sethi, G.
Ahn, K.
author_facet Zhang, J.
Sikka, S.
Siveen, K.
Lee, J.
Um, J.
Kumar, Alan Prem
Chinnathambi, A.
Alharbi, S.
Basappa
Rangappa, K.
Sethi, G.
Ahn, K.
author_sort Zhang, J.
building Curtin Institutional Repository
collection Online Access
description The pleiotropic transcription factor, signal transducer and activator of transcription 3 (STAT3) is often aberrantly activated in a wide variety of cancers and plays a pivotal role in tumor initiation, promotion and progression. Targeting deregulated STAT3 activation by small molecule inhibitors is generally considered as an important therapeutic strategy. Hence, in the present study, we evaluated the potential of cardamonin (CD), a 2',4'-dihydroxy-6'-methoxychalcone, to modulate STAT3 activation in prostate cancer (PC) cells and found that this chalcone can indeed exhibit significant anticancer effects through negatively regulating STAT3 activation by diverse molecular mechanism(s). CD suppressed STAT3 phosphorylation, nuclear translocation and DNA binding ability in PC cells. Computational modeling revealed that CD can bind directly to the Src Homology 2 domain of STAT3 and also effectively inhibit its dimerization. CD was also found to significantly reduce the migratory/invasive potential of PC cells through the downregulation of various oncogenic proteins. Overall, the data indicates that the potential application of CD as a STAT3 blocker can mitigate both the growth and survival of PC cells.
first_indexed 2025-11-14T09:45:52Z
format Journal Article
id curtin-20.500.11937-50872
institution Curtin University Malaysia
institution_category Local University
last_indexed 2025-11-14T09:45:52Z
publishDate 2017
publisher Springer
recordtype eprints
repository_type Digital Repository
spelling curtin-20.500.11937-508722017-09-13T15:49:51Z Cardamonin represses proliferation, invasion, and causes apoptosis through the modulation of signal transducer and activator of transcription 3 pathway in prostate cancer Zhang, J. Sikka, S. Siveen, K. Lee, J. Um, J. Kumar, Alan Prem Chinnathambi, A. Alharbi, S. Basappa Rangappa, K. Sethi, G. Ahn, K. The pleiotropic transcription factor, signal transducer and activator of transcription 3 (STAT3) is often aberrantly activated in a wide variety of cancers and plays a pivotal role in tumor initiation, promotion and progression. Targeting deregulated STAT3 activation by small molecule inhibitors is generally considered as an important therapeutic strategy. Hence, in the present study, we evaluated the potential of cardamonin (CD), a 2',4'-dihydroxy-6'-methoxychalcone, to modulate STAT3 activation in prostate cancer (PC) cells and found that this chalcone can indeed exhibit significant anticancer effects through negatively regulating STAT3 activation by diverse molecular mechanism(s). CD suppressed STAT3 phosphorylation, nuclear translocation and DNA binding ability in PC cells. Computational modeling revealed that CD can bind directly to the Src Homology 2 domain of STAT3 and also effectively inhibit its dimerization. CD was also found to significantly reduce the migratory/invasive potential of PC cells through the downregulation of various oncogenic proteins. Overall, the data indicates that the potential application of CD as a STAT3 blocker can mitigate both the growth and survival of PC cells. 2017 Journal Article http://hdl.handle.net/20.500.11937/50872 10.1007/s10495-016-1313-7 Springer restricted
spellingShingle Zhang, J.
Sikka, S.
Siveen, K.
Lee, J.
Um, J.
Kumar, Alan Prem
Chinnathambi, A.
Alharbi, S.
Basappa
Rangappa, K.
Sethi, G.
Ahn, K.
Cardamonin represses proliferation, invasion, and causes apoptosis through the modulation of signal transducer and activator of transcription 3 pathway in prostate cancer
title Cardamonin represses proliferation, invasion, and causes apoptosis through the modulation of signal transducer and activator of transcription 3 pathway in prostate cancer
title_full Cardamonin represses proliferation, invasion, and causes apoptosis through the modulation of signal transducer and activator of transcription 3 pathway in prostate cancer
title_fullStr Cardamonin represses proliferation, invasion, and causes apoptosis through the modulation of signal transducer and activator of transcription 3 pathway in prostate cancer
title_full_unstemmed Cardamonin represses proliferation, invasion, and causes apoptosis through the modulation of signal transducer and activator of transcription 3 pathway in prostate cancer
title_short Cardamonin represses proliferation, invasion, and causes apoptosis through the modulation of signal transducer and activator of transcription 3 pathway in prostate cancer
title_sort cardamonin represses proliferation, invasion, and causes apoptosis through the modulation of signal transducer and activator of transcription 3 pathway in prostate cancer
url http://hdl.handle.net/20.500.11937/50872