Electrochemically Induced Formation of Cytochrome c Oligomers at Soft Interfaces
The formation of cytochrome c oligomers was induced at liquid−gel and liquid−liquid interfaces via electroadsorption. At an optimum interfacial potential (Eads=0.975 V), the protein was accumulated at these soft interfaces. It was found that as the concentration of adsorbed protein increased, a sing...
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| Format: | Journal Article |
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Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
2017
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| Online Access: | http://hdl.handle.net/20.500.11937/50558 |
| _version_ | 1848758498398044160 |
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| author | Alvarez de Eulate, Eva O'Sullivan, S. Arrigan, Damien |
| author_facet | Alvarez de Eulate, Eva O'Sullivan, S. Arrigan, Damien |
| author_sort | Alvarez de Eulate, Eva |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The formation of cytochrome c oligomers was induced at liquid−gel and liquid−liquid interfaces via electroadsorption. At an optimum interfacial potential (Eads=0.975 V), the protein was accumulated at these soft interfaces. It was found that as the concentration of adsorbed protein increased, a single voltammetric peak evolved into double and triple peaks (tads=300 s). Analysis of the protein that accumulated at the interfaces by polyacrylamide gel electrophoresis indicated the presence of oligomeric species, corresponding to dimers (ca. 27 kD), trimers (ca. 35 kD), and even larger species (>250 kD) after prolonged electroadsorption (tads=2 h) at macro-scale soft interfaces. Accordingly, it was possible to electrochemically induce oligomerisation at these soft interfaces, which can be tuned through experimental factors such as interfacial potential difference, electroadsorption time, and bulk solution concentration. These results suggest the use of electrochemistry at soft interfaces as a strategy for the investigation of protein oligomerisation and its inhibition. |
| first_indexed | 2025-11-14T09:44:57Z |
| format | Journal Article |
| id | curtin-20.500.11937-50558 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:44:57Z |
| publishDate | 2017 |
| publisher | Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-505582018-02-28T05:51:20Z Electrochemically Induced Formation of Cytochrome c Oligomers at Soft Interfaces Alvarez de Eulate, Eva O'Sullivan, S. Arrigan, Damien The formation of cytochrome c oligomers was induced at liquid−gel and liquid−liquid interfaces via electroadsorption. At an optimum interfacial potential (Eads=0.975 V), the protein was accumulated at these soft interfaces. It was found that as the concentration of adsorbed protein increased, a single voltammetric peak evolved into double and triple peaks (tads=300 s). Analysis of the protein that accumulated at the interfaces by polyacrylamide gel electrophoresis indicated the presence of oligomeric species, corresponding to dimers (ca. 27 kD), trimers (ca. 35 kD), and even larger species (>250 kD) after prolonged electroadsorption (tads=2 h) at macro-scale soft interfaces. Accordingly, it was possible to electrochemically induce oligomerisation at these soft interfaces, which can be tuned through experimental factors such as interfacial potential difference, electroadsorption time, and bulk solution concentration. These results suggest the use of electrochemistry at soft interfaces as a strategy for the investigation of protein oligomerisation and its inhibition. 2017 Journal Article http://hdl.handle.net/20.500.11937/50558 10.1002/celc.201600851 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim fulltext |
| spellingShingle | Alvarez de Eulate, Eva O'Sullivan, S. Arrigan, Damien Electrochemically Induced Formation of Cytochrome c Oligomers at Soft Interfaces |
| title | Electrochemically Induced Formation of Cytochrome c Oligomers at Soft Interfaces |
| title_full | Electrochemically Induced Formation of Cytochrome c Oligomers at Soft Interfaces |
| title_fullStr | Electrochemically Induced Formation of Cytochrome c Oligomers at Soft Interfaces |
| title_full_unstemmed | Electrochemically Induced Formation of Cytochrome c Oligomers at Soft Interfaces |
| title_short | Electrochemically Induced Formation of Cytochrome c Oligomers at Soft Interfaces |
| title_sort | electrochemically induced formation of cytochrome c oligomers at soft interfaces |
| url | http://hdl.handle.net/20.500.11937/50558 |