Evaluating multilocus Bayesian species delimitation for discovery of cryptic mycorrhizal diversity
The increasing availability of DNA sequence data enables exciting new opportunities for fungal ecology. However, it amplifies the challenge of how to objectively classify the diversity of fungal sequences into meaningful units, often in the absence of morphological characters. Here, we test the util...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11937/50227 |
| _version_ | 1848758426352484352 |
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| author | Whitehead, M. Catullo, R. Ruibal, M. Dixon, Kingsley Peakall, R. Linde, C. |
| author_facet | Whitehead, M. Catullo, R. Ruibal, M. Dixon, Kingsley Peakall, R. Linde, C. |
| author_sort | Whitehead, M. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The increasing availability of DNA sequence data enables exciting new opportunities for fungal ecology. However, it amplifies the challenge of how to objectively classify the diversity of fungal sequences into meaningful units, often in the absence of morphological characters. Here, we test the utility of modern multilocus Bayesian coalescent-based methods for delimiting cryptic fungal diversity in the orchid mycorrhiza morphospecies Serendipita vermifera. We obtained 147 fungal isolates from Caladenia, a speciose clade of Australian orchids known to associate with Serendipita fungi. DNA sequence data for 7 nuclear and mtDNA loci were used to erect competing species hypotheses by clustering isolates based on: (a) ITS sequence divergence, (b) Bayesian admixture analysis, and (c) mtDNA variation. We implemented two coalescent-based Bayesian methods to determine which species hypothesis best fitted our data. Both methods found strong support for eight species of Serendipita among our isolates, supporting species boundaries reflected in ITS divergence. Patterns of host plant association showed evidence for both generalist and specialist associations within the host genus Caladenia. Our findings demonstrate the utility of Bayesian species delimitation methods and suggest that wider application of these techniques will readily uncover new species in other cryptic fungal lineages. |
| first_indexed | 2025-11-14T09:43:48Z |
| format | Journal Article |
| id | curtin-20.500.11937-50227 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:43:48Z |
| publishDate | 2017 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-502272017-09-13T15:41:23Z Evaluating multilocus Bayesian species delimitation for discovery of cryptic mycorrhizal diversity Whitehead, M. Catullo, R. Ruibal, M. Dixon, Kingsley Peakall, R. Linde, C. The increasing availability of DNA sequence data enables exciting new opportunities for fungal ecology. However, it amplifies the challenge of how to objectively classify the diversity of fungal sequences into meaningful units, often in the absence of morphological characters. Here, we test the utility of modern multilocus Bayesian coalescent-based methods for delimiting cryptic fungal diversity in the orchid mycorrhiza morphospecies Serendipita vermifera. We obtained 147 fungal isolates from Caladenia, a speciose clade of Australian orchids known to associate with Serendipita fungi. DNA sequence data for 7 nuclear and mtDNA loci were used to erect competing species hypotheses by clustering isolates based on: (a) ITS sequence divergence, (b) Bayesian admixture analysis, and (c) mtDNA variation. We implemented two coalescent-based Bayesian methods to determine which species hypothesis best fitted our data. Both methods found strong support for eight species of Serendipita among our isolates, supporting species boundaries reflected in ITS divergence. Patterns of host plant association showed evidence for both generalist and specialist associations within the host genus Caladenia. Our findings demonstrate the utility of Bayesian species delimitation methods and suggest that wider application of these techniques will readily uncover new species in other cryptic fungal lineages. 2017 Journal Article http://hdl.handle.net/20.500.11937/50227 10.1016/j.funeco.2016.11.009 restricted |
| spellingShingle | Whitehead, M. Catullo, R. Ruibal, M. Dixon, Kingsley Peakall, R. Linde, C. Evaluating multilocus Bayesian species delimitation for discovery of cryptic mycorrhizal diversity |
| title | Evaluating multilocus Bayesian species delimitation for discovery of cryptic mycorrhizal diversity |
| title_full | Evaluating multilocus Bayesian species delimitation for discovery of cryptic mycorrhizal diversity |
| title_fullStr | Evaluating multilocus Bayesian species delimitation for discovery of cryptic mycorrhizal diversity |
| title_full_unstemmed | Evaluating multilocus Bayesian species delimitation for discovery of cryptic mycorrhizal diversity |
| title_short | Evaluating multilocus Bayesian species delimitation for discovery of cryptic mycorrhizal diversity |
| title_sort | evaluating multilocus bayesian species delimitation for discovery of cryptic mycorrhizal diversity |
| url | http://hdl.handle.net/20.500.11937/50227 |