Cytochemistry of leukocytes from the family Macropodidae

The cytochemical staining characteristics of leukocytes from four species of Macropodidae and one species of Potoroidae were studied to investigate the cellular composition of leukocytes within the Macropodidae family and determine markers that may be useful for identifying cell lineage. Blood smear...

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Main Authors: Hulme-Moir, K., Clark, Phillip
Format: Journal Article
Published: Springer 2012
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/49348
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author Hulme-Moir, K.
Clark, Phillip
author_facet Hulme-Moir, K.
Clark, Phillip
author_sort Hulme-Moir, K.
building Curtin Institutional Repository
collection Online Access
description The cytochemical staining characteristics of leukocytes from four species of Macropodidae and one species of Potoroidae were studied to investigate the cellular composition of leukocytes within the Macropodidae family and determine markers that may be useful for identifying cell lineage. Blood smears from the tammar wallaby (Macropus eugenii), the western grey kangaroo (Macropus fuliginosis), the red kangaroo (Macropus rufus), quokka (Setonix brachyurus) and a potoroid species, the woylie (Bettongia pencillata) were examined following reaction for Sudan Black B (SBB), peroxidase (PER), chloracetate esterase (CAE), a-naphthyl butyrate esterase (NBE) and alkaline phosphatase (ALP). Similar to domestic animal species, the neutrophils and eosinophils of macropodid species stained for both SBB and PER while monocytes and lymphocytes showed little or no reaction. CAE and NBE, however, were not useful as markers for macropodid neutrophils and monocytes, respectively. Significant variation between species was seen in ALP content.
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spelling curtin-20.500.11937-493482018-05-02T03:57:54Z Cytochemistry of leukocytes from the family Macropodidae Hulme-Moir, K. Clark, Phillip Marsupial . Macropodidae . Leukocytes . Cytochemistry The cytochemical staining characteristics of leukocytes from four species of Macropodidae and one species of Potoroidae were studied to investigate the cellular composition of leukocytes within the Macropodidae family and determine markers that may be useful for identifying cell lineage. Blood smears from the tammar wallaby (Macropus eugenii), the western grey kangaroo (Macropus fuliginosis), the red kangaroo (Macropus rufus), quokka (Setonix brachyurus) and a potoroid species, the woylie (Bettongia pencillata) were examined following reaction for Sudan Black B (SBB), peroxidase (PER), chloracetate esterase (CAE), a-naphthyl butyrate esterase (NBE) and alkaline phosphatase (ALP). Similar to domestic animal species, the neutrophils and eosinophils of macropodid species stained for both SBB and PER while monocytes and lymphocytes showed little or no reaction. CAE and NBE, however, were not useful as markers for macropodid neutrophils and monocytes, respectively. Significant variation between species was seen in ALP content. 2012 Journal Article http://hdl.handle.net/20.500.11937/49348 10.1007/s00580-010-1071-9 Springer restricted
spellingShingle Marsupial . Macropodidae . Leukocytes . Cytochemistry
Hulme-Moir, K.
Clark, Phillip
Cytochemistry of leukocytes from the family Macropodidae
title Cytochemistry of leukocytes from the family Macropodidae
title_full Cytochemistry of leukocytes from the family Macropodidae
title_fullStr Cytochemistry of leukocytes from the family Macropodidae
title_full_unstemmed Cytochemistry of leukocytes from the family Macropodidae
title_short Cytochemistry of leukocytes from the family Macropodidae
title_sort cytochemistry of leukocytes from the family macropodidae
topic Marsupial . Macropodidae . Leukocytes . Cytochemistry
url http://hdl.handle.net/20.500.11937/49348