Review of fluorescent standards for calibration of in situ fluorometers: Recommendationsapplied in coastal and ocean observing programs
Fluorometers are widely used in ecosystem observing to monitor fluorescence signals from organic compounds, as well as to infer geophysical parameters such as chlorophyll or CDOM concentration, butmeasurements are susceptible to variation caused by biofouling, instrument design, sensor drift, operat...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Published: |
Optical Society of America
2011
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| Online Access: | http://hdl.handle.net/20.500.11937/49311 |
| _version_ | 1848758213027037184 |
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| author | Earp, A. Hanson, C. Ralph, P. Brando, V. Allen, S. Baird, M. Clementson, L. Daniel, P. Dekker, A. Fearns, Peter Parslow, J. Strutton, P. Thompson, P. Underwood, M. Weeks, S. Doblin, M. |
| author_facet | Earp, A. Hanson, C. Ralph, P. Brando, V. Allen, S. Baird, M. Clementson, L. Daniel, P. Dekker, A. Fearns, Peter Parslow, J. Strutton, P. Thompson, P. Underwood, M. Weeks, S. Doblin, M. |
| author_sort | Earp, A. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Fluorometers are widely used in ecosystem observing to monitor fluorescence signals from organic compounds, as well as to infer geophysical parameters such as chlorophyll or CDOM concentration, butmeasurements are susceptible to variation caused by biofouling, instrument design, sensor drift, operating environment, and calibration rigor. To collect high quality data, such sensors need frequent checking and regular calibration. In this study, a wide variety of both liquid and solid fluorescentmaterials were trialed to assess their suitability as reference standards for performance assessment of in situ fluorometers. Criteria used to evaluate the standards included the spectral excitation/emission responses of the materials relative to fluorescence sensors and to targeted ocean properties, the linearity of the fluorometer’s optical response with increasing concentration, stability and consistency, availability and ease of use, as well as cost. Findings are summarized as a series of recommended reference standards for sensors deployed on stationary and mobile platforms, to suit avariety of in situ coastal to ocean sensor configurations. Repeated determinations of chlorophyll scale factor using the recommended liquid standard, Fluorescein, achieved an accuracy of 2.5%. Repeatedmeasurements with the recommended solid standard, Plexiglas Satinice® plum 4H01 DC (polymethylmethacrylate), over an 18 day period varied from the mean value by 1.0% for chlorophyll sensors and 3.3% for CDOM sensors. |
| first_indexed | 2025-11-14T09:40:24Z |
| format | Journal Article |
| id | curtin-20.500.11937-49311 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:40:24Z |
| publishDate | 2011 |
| publisher | Optical Society of America |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-493112017-03-15T22:56:20Z Review of fluorescent standards for calibration of in situ fluorometers: Recommendationsapplied in coastal and ocean observing programs Earp, A. Hanson, C. Ralph, P. Brando, V. Allen, S. Baird, M. Clementson, L. Daniel, P. Dekker, A. Fearns, Peter Parslow, J. Strutton, P. Thompson, P. Underwood, M. Weeks, S. Doblin, M. Fluorometers are widely used in ecosystem observing to monitor fluorescence signals from organic compounds, as well as to infer geophysical parameters such as chlorophyll or CDOM concentration, butmeasurements are susceptible to variation caused by biofouling, instrument design, sensor drift, operating environment, and calibration rigor. To collect high quality data, such sensors need frequent checking and regular calibration. In this study, a wide variety of both liquid and solid fluorescentmaterials were trialed to assess their suitability as reference standards for performance assessment of in situ fluorometers. Criteria used to evaluate the standards included the spectral excitation/emission responses of the materials relative to fluorescence sensors and to targeted ocean properties, the linearity of the fluorometer’s optical response with increasing concentration, stability and consistency, availability and ease of use, as well as cost. Findings are summarized as a series of recommended reference standards for sensors deployed on stationary and mobile platforms, to suit avariety of in situ coastal to ocean sensor configurations. Repeated determinations of chlorophyll scale factor using the recommended liquid standard, Fluorescein, achieved an accuracy of 2.5%. Repeatedmeasurements with the recommended solid standard, Plexiglas Satinice® plum 4H01 DC (polymethylmethacrylate), over an 18 day period varied from the mean value by 1.0% for chlorophyll sensors and 3.3% for CDOM sensors. 2011 Journal Article http://hdl.handle.net/20.500.11937/49311 Optical Society of America restricted |
| spellingShingle | Earp, A. Hanson, C. Ralph, P. Brando, V. Allen, S. Baird, M. Clementson, L. Daniel, P. Dekker, A. Fearns, Peter Parslow, J. Strutton, P. Thompson, P. Underwood, M. Weeks, S. Doblin, M. Review of fluorescent standards for calibration of in situ fluorometers: Recommendationsapplied in coastal and ocean observing programs |
| title | Review of fluorescent standards for calibration of in situ fluorometers: Recommendationsapplied in coastal and ocean observing programs |
| title_full | Review of fluorescent standards for calibration of in situ fluorometers: Recommendationsapplied in coastal and ocean observing programs |
| title_fullStr | Review of fluorescent standards for calibration of in situ fluorometers: Recommendationsapplied in coastal and ocean observing programs |
| title_full_unstemmed | Review of fluorescent standards for calibration of in situ fluorometers: Recommendationsapplied in coastal and ocean observing programs |
| title_short | Review of fluorescent standards for calibration of in situ fluorometers: Recommendationsapplied in coastal and ocean observing programs |
| title_sort | review of fluorescent standards for calibration of in situ fluorometers: recommendationsapplied in coastal and ocean observing programs |
| url | http://hdl.handle.net/20.500.11937/49311 |