Phospholipase C?1 is required for metastasis development and progression

Cell motility and invasion play an essential role in the development of metastasis. Evidence suggests that the enzyme phospholipase C?1 (PLC?1) may be involved in tumor progression and possibly development of metastasis. In this study, we show that down-regulation of PLC?1 expression severely impair...

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Main Authors: Sala, G., Dituri, F., Raimondi, C., Previdi, S., Maffucci, T., Mazzoletti, M., Rossi, C., Iezzi, M., Lattanzio, R., Piantelli, M., Iacobelli, S., Broggini, M., Falasca, Marco
Format: Journal Article
Published: American association for Cancer Research 2008
Online Access:http://hdl.handle.net/20.500.11937/48174
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author Sala, G.
Dituri, F.
Raimondi, C.
Previdi, S.
Maffucci, T.
Mazzoletti, M.
Rossi, C.
Iezzi, M.
Lattanzio, R.
Piantelli, M.
Iacobelli, S.
Broggini, M.
Falasca, Marco
author_facet Sala, G.
Dituri, F.
Raimondi, C.
Previdi, S.
Maffucci, T.
Mazzoletti, M.
Rossi, C.
Iezzi, M.
Lattanzio, R.
Piantelli, M.
Iacobelli, S.
Broggini, M.
Falasca, Marco
author_sort Sala, G.
building Curtin Institutional Repository
collection Online Access
description Cell motility and invasion play an essential role in the development of metastasis. Evidence suggests that the enzyme phospholipase C?1 (PLC?1) may be involved in tumor progression and possibly development of metastasis. In this study, we show that down-regulation of PLC?1 expression severely impairs activation of the small GTP-binding protein Rac and cell invasion in breast cancer cell lines and U87 in vitro. Experimental metastasis assays in nude mice show that inducible knockdown of PLC?1 strongly inhibits development of MDA-MB-231-derived lung metastasis and reverts metastasis formation. In addition, analysis of 60 breast cancer patients' tissues revealed an increase of PLC?1 expression in metastasis compared with the primary tumor in 50% of tissues analyzed. These data showa critical role of PLC?1 in the metastatic potential of cancer cells, and they further indicate that PLC?1 inhibition has a therapeutic potential in the treatment of metastasis dissemination. ©2008 American Association for Cancer Research.
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format Journal Article
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institution Curtin University Malaysia
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last_indexed 2025-11-14T09:37:37Z
publishDate 2008
publisher American association for Cancer Research
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spelling curtin-20.500.11937-481742017-09-13T14:22:15Z Phospholipase C?1 is required for metastasis development and progression Sala, G. Dituri, F. Raimondi, C. Previdi, S. Maffucci, T. Mazzoletti, M. Rossi, C. Iezzi, M. Lattanzio, R. Piantelli, M. Iacobelli, S. Broggini, M. Falasca, Marco Cell motility and invasion play an essential role in the development of metastasis. Evidence suggests that the enzyme phospholipase C?1 (PLC?1) may be involved in tumor progression and possibly development of metastasis. In this study, we show that down-regulation of PLC?1 expression severely impairs activation of the small GTP-binding protein Rac and cell invasion in breast cancer cell lines and U87 in vitro. Experimental metastasis assays in nude mice show that inducible knockdown of PLC?1 strongly inhibits development of MDA-MB-231-derived lung metastasis and reverts metastasis formation. In addition, analysis of 60 breast cancer patients' tissues revealed an increase of PLC?1 expression in metastasis compared with the primary tumor in 50% of tissues analyzed. These data showa critical role of PLC?1 in the metastatic potential of cancer cells, and they further indicate that PLC?1 inhibition has a therapeutic potential in the treatment of metastasis dissemination. ©2008 American Association for Cancer Research. 2008 Journal Article http://hdl.handle.net/20.500.11937/48174 10.1158/0008-5472.CAN-08-1181 American association for Cancer Research unknown
spellingShingle Sala, G.
Dituri, F.
Raimondi, C.
Previdi, S.
Maffucci, T.
Mazzoletti, M.
Rossi, C.
Iezzi, M.
Lattanzio, R.
Piantelli, M.
Iacobelli, S.
Broggini, M.
Falasca, Marco
Phospholipase C?1 is required for metastasis development and progression
title Phospholipase C?1 is required for metastasis development and progression
title_full Phospholipase C?1 is required for metastasis development and progression
title_fullStr Phospholipase C?1 is required for metastasis development and progression
title_full_unstemmed Phospholipase C?1 is required for metastasis development and progression
title_short Phospholipase C?1 is required for metastasis development and progression
title_sort phospholipase c?1 is required for metastasis development and progression
url http://hdl.handle.net/20.500.11937/48174