Effect of carbon disulfide on pyrogallol-luminol auto-oxidation chemiluminescence system and superoxide anion

OBJECTIVE: To study the effect of carbon disulfide (CS(2)) on pyrogallol-luminol auto-oxidation chemiluminescence (PA-L) system and production of superoxide anion. METHODS: Luminescence dynamic curve of CS(2) and inhibition effect of superoxide dismutase (SOD) on intensity of chemiluminescence induc...

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Main Authors: Jian, Le, Chen, Q.
Format: Journal Article
Published: Chinese Medical Association 2000
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/47578
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author Jian, Le
Chen, Q.
author_facet Jian, Le
Chen, Q.
author_sort Jian, Le
building Curtin Institutional Repository
collection Online Access
description OBJECTIVE: To study the effect of carbon disulfide (CS(2)) on pyrogallol-luminol auto-oxidation chemiluminescence (PA-L) system and production of superoxide anion. METHODS: Luminescence dynamic curve of CS(2) and inhibition effect of superoxide dismutase (SOD) on intensity of chemiluminescence induced by CS(2) were studied with PA-L chemiluminescence system. RESULTS: Different concentrations of CS(2) (10, 40, 80 mg/ml) could enhance the emission intensity of chemiluminescence of PA-L system and delayed its peak time, with a significant dose-effect relationship, as compared with that of solvent ethanol. Peak of chemiluminescence induced by 80 mg/ml of CS(2) was higher than its background value without solvent ethanol. Chemiluminescence induced by CS(2) could be inhibited by SOD. CONCLUSION: CS(2) can generate superoxide anion in PA-L system and delay the peak time of chemiluminescence emission dynamic curve.
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institution Curtin University Malaysia
institution_category Local University
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publishDate 2000
publisher Chinese Medical Association
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spelling curtin-20.500.11937-475782017-01-30T15:34:17Z Effect of carbon disulfide on pyrogallol-luminol auto-oxidation chemiluminescence system and superoxide anion Jian, Le Chen, Q. Carbon disulfide Pyrogallol Anions Superoxide dismutase Chemiluminescence OBJECTIVE: To study the effect of carbon disulfide (CS(2)) on pyrogallol-luminol auto-oxidation chemiluminescence (PA-L) system and production of superoxide anion. METHODS: Luminescence dynamic curve of CS(2) and inhibition effect of superoxide dismutase (SOD) on intensity of chemiluminescence induced by CS(2) were studied with PA-L chemiluminescence system. RESULTS: Different concentrations of CS(2) (10, 40, 80 mg/ml) could enhance the emission intensity of chemiluminescence of PA-L system and delayed its peak time, with a significant dose-effect relationship, as compared with that of solvent ethanol. Peak of chemiluminescence induced by 80 mg/ml of CS(2) was higher than its background value without solvent ethanol. Chemiluminescence induced by CS(2) could be inhibited by SOD. CONCLUSION: CS(2) can generate superoxide anion in PA-L system and delay the peak time of chemiluminescence emission dynamic curve. 2000 Journal Article http://hdl.handle.net/20.500.11937/47578 Chinese Medical Association restricted
spellingShingle Carbon disulfide
Pyrogallol
Anions
Superoxide dismutase
Chemiluminescence
Jian, Le
Chen, Q.
Effect of carbon disulfide on pyrogallol-luminol auto-oxidation chemiluminescence system and superoxide anion
title Effect of carbon disulfide on pyrogallol-luminol auto-oxidation chemiluminescence system and superoxide anion
title_full Effect of carbon disulfide on pyrogallol-luminol auto-oxidation chemiluminescence system and superoxide anion
title_fullStr Effect of carbon disulfide on pyrogallol-luminol auto-oxidation chemiluminescence system and superoxide anion
title_full_unstemmed Effect of carbon disulfide on pyrogallol-luminol auto-oxidation chemiluminescence system and superoxide anion
title_short Effect of carbon disulfide on pyrogallol-luminol auto-oxidation chemiluminescence system and superoxide anion
title_sort effect of carbon disulfide on pyrogallol-luminol auto-oxidation chemiluminescence system and superoxide anion
topic Carbon disulfide
Pyrogallol
Anions
Superoxide dismutase
Chemiluminescence
url http://hdl.handle.net/20.500.11937/47578