Mannosylation of mutated MBP83-99 peptides diverts immune responses from Th1 to Th2
Multiple sclerosis (MS) is an autoimmune demyelinating disease mediated primarily by CD4+ T cells. The design of peptide mutants of disease-associated myelin epitopes to alter immune responses offers a promising avenue for the treatment of MS. We designed and synthesized a number of peptide analogs...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Published: |
2008
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| Online Access: | http://hdl.handle.net/20.500.11937/47052 |
| _version_ | 1848757728853360640 |
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| author | Katsara, M. Yuriev, E. Ramsland, Paul Deraos, G. Tselios, T. Matsoukas, J. Apostolopoulos, V. |
| author_facet | Katsara, M. Yuriev, E. Ramsland, Paul Deraos, G. Tselios, T. Matsoukas, J. Apostolopoulos, V. |
| author_sort | Katsara, M. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Multiple sclerosis (MS) is an autoimmune demyelinating disease mediated primarily by CD4+ T cells. The design of peptide mutants of disease-associated myelin epitopes to alter immune responses offers a promising avenue for the treatment of MS. We designed and synthesized a number of peptide analogs by mutating the principal TCR contact residue based on MBP83-99 epitope and these peptides were conjugated to reduced mannan. Immune responses were diverted from Th1 to Th2 in SJL/J mice and generated antibodies which did not cross-react with native MBP protein. Peptide [Y91]MBP83-99 gave the best cytokine and antibody profile and constitutes a promising candidate peptide for immunotherapy of MS. Structural alignment of existing crystal structures revealed the peptide binding motif of I-As. Molecular modeling was used to identify H-bonding and van der Waals interactions between peptides and MHC (I-As). © 2008 Elsevier Ltd. All rights reserved. |
| first_indexed | 2025-11-14T09:32:43Z |
| format | Journal Article |
| id | curtin-20.500.11937-47052 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:32:43Z |
| publishDate | 2008 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-470522017-09-13T14:27:26Z Mannosylation of mutated MBP83-99 peptides diverts immune responses from Th1 to Th2 Katsara, M. Yuriev, E. Ramsland, Paul Deraos, G. Tselios, T. Matsoukas, J. Apostolopoulos, V. Multiple sclerosis (MS) is an autoimmune demyelinating disease mediated primarily by CD4+ T cells. The design of peptide mutants of disease-associated myelin epitopes to alter immune responses offers a promising avenue for the treatment of MS. We designed and synthesized a number of peptide analogs by mutating the principal TCR contact residue based on MBP83-99 epitope and these peptides were conjugated to reduced mannan. Immune responses were diverted from Th1 to Th2 in SJL/J mice and generated antibodies which did not cross-react with native MBP protein. Peptide [Y91]MBP83-99 gave the best cytokine and antibody profile and constitutes a promising candidate peptide for immunotherapy of MS. Structural alignment of existing crystal structures revealed the peptide binding motif of I-As. Molecular modeling was used to identify H-bonding and van der Waals interactions between peptides and MHC (I-As). © 2008 Elsevier Ltd. All rights reserved. 2008 Journal Article http://hdl.handle.net/20.500.11937/47052 10.1016/j.molimm.2008.04.024 restricted |
| spellingShingle | Katsara, M. Yuriev, E. Ramsland, Paul Deraos, G. Tselios, T. Matsoukas, J. Apostolopoulos, V. Mannosylation of mutated MBP83-99 peptides diverts immune responses from Th1 to Th2 |
| title | Mannosylation of mutated MBP83-99 peptides diverts immune responses from Th1 to Th2 |
| title_full | Mannosylation of mutated MBP83-99 peptides diverts immune responses from Th1 to Th2 |
| title_fullStr | Mannosylation of mutated MBP83-99 peptides diverts immune responses from Th1 to Th2 |
| title_full_unstemmed | Mannosylation of mutated MBP83-99 peptides diverts immune responses from Th1 to Th2 |
| title_short | Mannosylation of mutated MBP83-99 peptides diverts immune responses from Th1 to Th2 |
| title_sort | mannosylation of mutated mbp83-99 peptides diverts immune responses from th1 to th2 |
| url | http://hdl.handle.net/20.500.11937/47052 |