Toll-like receptor (TLR) expression on CD4+ and CD8+ T-cells in patients chronically infected with hepatitis C virus

Toll-like receptor (TLR) expression on T-cells and the signalling pathways that lead to the production of cytokines may limit antigen-specific T-cell responses. Here, expression of TLR and retinoic acid inducible gene I (RIG-I) on T-cells were evaluated in patients chronically infected with hepatiti...

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Main Authors: Hammond, T., Lee, S., Watson, M., Flexman, J., Cheng, W., Fernandez, S., Price, Patricia
Format: Journal Article
Published: 2010
Online Access:http://hdl.handle.net/20.500.11937/47028
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author Hammond, T.
Lee, S.
Watson, M.
Flexman, J.
Cheng, W.
Fernandez, S.
Price, Patricia
author_facet Hammond, T.
Lee, S.
Watson, M.
Flexman, J.
Cheng, W.
Fernandez, S.
Price, Patricia
author_sort Hammond, T.
building Curtin Institutional Repository
collection Online Access
description Toll-like receptor (TLR) expression on T-cells and the signalling pathways that lead to the production of cytokines may limit antigen-specific T-cell responses. Here, expression of TLR and retinoic acid inducible gene I (RIG-I) on T-cells were evaluated in patients chronically infected with hepatitis C virus (HCV), before and during pegylated interferon-a and ribavirin therapy. Expression of TLR2,3,4,7,9 and retinoic acid inducible gene (RIG)-I on different CD4+ and CD8+ T-cell sub-populations (naïve: CD45RA+CD57-; central memory: TCM CD45RA-CD57-; effector memory: TEM CD45RA-CD57+ and terminally differentiated effector memory: TEMRA CD45RA+CD57+) were measured by flow cytometry. TLR7, TLR9 and RIG-I expression on CD4+ T-cells and RIG-I expression on CD8+ T-cells was higher in patients than healthy controls. Therapy increased expression of TLR2, TLR4 and TLR9 and this was observed for all T-cell sub-populations. Evaluation of TLR expression at baseline did not identify patients able to achieve sustained virological response following therapy. © 2010.
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spelling curtin-20.500.11937-470282017-09-13T14:28:07Z Toll-like receptor (TLR) expression on CD4+ and CD8+ T-cells in patients chronically infected with hepatitis C virus Hammond, T. Lee, S. Watson, M. Flexman, J. Cheng, W. Fernandez, S. Price, Patricia Toll-like receptor (TLR) expression on T-cells and the signalling pathways that lead to the production of cytokines may limit antigen-specific T-cell responses. Here, expression of TLR and retinoic acid inducible gene I (RIG-I) on T-cells were evaluated in patients chronically infected with hepatitis C virus (HCV), before and during pegylated interferon-a and ribavirin therapy. Expression of TLR2,3,4,7,9 and retinoic acid inducible gene (RIG)-I on different CD4+ and CD8+ T-cell sub-populations (naïve: CD45RA+CD57-; central memory: TCM CD45RA-CD57-; effector memory: TEM CD45RA-CD57+ and terminally differentiated effector memory: TEMRA CD45RA+CD57+) were measured by flow cytometry. TLR7, TLR9 and RIG-I expression on CD4+ T-cells and RIG-I expression on CD8+ T-cells was higher in patients than healthy controls. Therapy increased expression of TLR2, TLR4 and TLR9 and this was observed for all T-cell sub-populations. Evaluation of TLR expression at baseline did not identify patients able to achieve sustained virological response following therapy. © 2010. 2010 Journal Article http://hdl.handle.net/20.500.11937/47028 10.1016/j.cellimm.2010.06.001 restricted
spellingShingle Hammond, T.
Lee, S.
Watson, M.
Flexman, J.
Cheng, W.
Fernandez, S.
Price, Patricia
Toll-like receptor (TLR) expression on CD4+ and CD8+ T-cells in patients chronically infected with hepatitis C virus
title Toll-like receptor (TLR) expression on CD4+ and CD8+ T-cells in patients chronically infected with hepatitis C virus
title_full Toll-like receptor (TLR) expression on CD4+ and CD8+ T-cells in patients chronically infected with hepatitis C virus
title_fullStr Toll-like receptor (TLR) expression on CD4+ and CD8+ T-cells in patients chronically infected with hepatitis C virus
title_full_unstemmed Toll-like receptor (TLR) expression on CD4+ and CD8+ T-cells in patients chronically infected with hepatitis C virus
title_short Toll-like receptor (TLR) expression on CD4+ and CD8+ T-cells in patients chronically infected with hepatitis C virus
title_sort toll-like receptor (tlr) expression on cd4+ and cd8+ t-cells in patients chronically infected with hepatitis c virus
url http://hdl.handle.net/20.500.11937/47028