High levels of malic acid production by the bioconversion of corn straw hydrolyte using an isolated Rhizopus delemar strain
The microbial fermentation of malic acid, which is one of the most important organic acid platforms used widely in food and chemical engineering, has attracted considerable interest. A malate production strain was isolated, a mutation was induced, and regulation of the metabolic network was then con...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal Article |
| Published: |
Korean Society for Biotechnology and Bioengineering
2014
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/46759 |
| _version_ | 1848757649967939584 |
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| author | Li, Xingjiang Liu, Y. Yang, Y. Zhang, H. Wang, H. Wu, Y. Zhang, M. Sun, T. Cheng, J. Wu, X. Pan, L. Jiang, S. Wu, Hongwei |
| author_facet | Li, Xingjiang Liu, Y. Yang, Y. Zhang, H. Wang, H. Wu, Y. Zhang, M. Sun, T. Cheng, J. Wu, X. Pan, L. Jiang, S. Wu, Hongwei |
| author_sort | Li, Xingjiang |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The microbial fermentation of malic acid, which is one of the most important organic acid platforms used widely in food and chemical engineering, has attracted considerable interest. A malate production strain was isolated, a mutation was induced, and regulation of the metabolic network was then conducted. The identification results showed that the malic acid production strain, HF- 119, belonged to Rhizopus delemar. An analysis of the metabolic pathway showed that the malic acid flux of this strain occurred through three main pathways, and many byproducts, such as succinic acid, fumaric acid and ethanol, were produced. Although corn straw hydrolyte was used, the metabolism of xylose was not as rapid as that of glucose. Subsequently, breeding of the strains and regulation of the metabolic network resulted in an increase in malate yield, and the strain HF-121 produced more than 120 g/L malic acid within 60 h. The ability to produce malic acid from biomass hydrolyte highlights the industrial development potential of this strain. |
| first_indexed | 2025-11-14T09:31:27Z |
| format | Journal Article |
| id | curtin-20.500.11937-46759 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:31:27Z |
| publishDate | 2014 |
| publisher | Korean Society for Biotechnology and Bioengineering |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-467592017-09-13T14:08:48Z High levels of malic acid production by the bioconversion of corn straw hydrolyte using an isolated Rhizopus delemar strain Li, Xingjiang Liu, Y. Yang, Y. Zhang, H. Wang, H. Wu, Y. Zhang, M. Sun, T. Cheng, J. Wu, X. Pan, L. Jiang, S. Wu, Hongwei metabolic network Rhizopus Delemar biomass hydrolyte malic acid The microbial fermentation of malic acid, which is one of the most important organic acid platforms used widely in food and chemical engineering, has attracted considerable interest. A malate production strain was isolated, a mutation was induced, and regulation of the metabolic network was then conducted. The identification results showed that the malic acid production strain, HF- 119, belonged to Rhizopus delemar. An analysis of the metabolic pathway showed that the malic acid flux of this strain occurred through three main pathways, and many byproducts, such as succinic acid, fumaric acid and ethanol, were produced. Although corn straw hydrolyte was used, the metabolism of xylose was not as rapid as that of glucose. Subsequently, breeding of the strains and regulation of the metabolic network resulted in an increase in malate yield, and the strain HF-121 produced more than 120 g/L malic acid within 60 h. The ability to produce malic acid from biomass hydrolyte highlights the industrial development potential of this strain. 2014 Journal Article http://hdl.handle.net/20.500.11937/46759 10.1007/s12257-014-0047-z Korean Society for Biotechnology and Bioengineering restricted |
| spellingShingle | metabolic network Rhizopus Delemar biomass hydrolyte malic acid Li, Xingjiang Liu, Y. Yang, Y. Zhang, H. Wang, H. Wu, Y. Zhang, M. Sun, T. Cheng, J. Wu, X. Pan, L. Jiang, S. Wu, Hongwei High levels of malic acid production by the bioconversion of corn straw hydrolyte using an isolated Rhizopus delemar strain |
| title | High levels of malic acid production by the bioconversion of corn straw hydrolyte using an isolated Rhizopus delemar strain |
| title_full | High levels of malic acid production by the bioconversion of corn straw hydrolyte using an isolated Rhizopus delemar strain |
| title_fullStr | High levels of malic acid production by the bioconversion of corn straw hydrolyte using an isolated Rhizopus delemar strain |
| title_full_unstemmed | High levels of malic acid production by the bioconversion of corn straw hydrolyte using an isolated Rhizopus delemar strain |
| title_short | High levels of malic acid production by the bioconversion of corn straw hydrolyte using an isolated Rhizopus delemar strain |
| title_sort | high levels of malic acid production by the bioconversion of corn straw hydrolyte using an isolated rhizopus delemar strain |
| topic | metabolic network Rhizopus Delemar biomass hydrolyte malic acid |
| url | http://hdl.handle.net/20.500.11937/46759 |