Cousins, siblings, or copies: the genomics of recurrent Staphylococcus aureus infections in chronic rhinosinusitis
BackgroundStaphylococcus aureus infection is known to play a role in recalcitrant chronic rhinosinusitis (CRS). However, it is unknown if recurrent S. aureus infections are caused by the same strain or are due to independent acquisitions of different strains.MethodsSamples were collected from patien...
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| Format: | Journal Article |
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Wiley-Blackwell
2014
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| Online Access: | http://hdl.handle.net/20.500.11937/46660 |
| _version_ | 1848757621595570176 |
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| author | Drilling, A. Coombs, Geoffrey Tan, H. Pearson, J. Boase, S. Psaltis, A. Speck, P. Vreugde, S. Wormald, P. |
| author_facet | Drilling, A. Coombs, Geoffrey Tan, H. Pearson, J. Boase, S. Psaltis, A. Speck, P. Vreugde, S. Wormald, P. |
| author_sort | Drilling, A. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | BackgroundStaphylococcus aureus infection is known to play a role in recalcitrant chronic rhinosinusitis (CRS). However, it is unknown if recurrent S. aureus infections are caused by the same strain or are due to independent acquisitions of different strains.MethodsSamples were collected from patients with CRS from July 2011 to August 2012. S. aureus was isolated from mucosal swabs and tissue specimens from patients who underwent surgery during the study period, or from swabs of areas of purulence taken in the postoperative period under endoscopic guidance. Pulsed-field gel electrophoresis was used to characterize S. aureus isolates.ResultsThirty-four patients were included in the study; 79% showed persistence of the same S. aureus strain in their paranasal sinuses (p = 0.001; H1 ? 50%). Furthermore, a significantly high frequency of patients with known biofilm status were positive for S. aureus biofilm (p = 0.002; H1 ? 50%). When patients were stratified according to disease evolution postsurgery, certain strains appeared to be more commonly associated with symptom persistence.ConclusionThe same S. aureus strain appears to persist in the paranasal sinuses of CRS patients despite multiple courses of culture-directed antibiotics. This suggests that conventional antimicrobial therapies in patients with CRS may not eliminate the organism. This may be partly explained by the formation of biofilms in the paranasal sinus region. |
| first_indexed | 2025-11-14T09:31:00Z |
| format | Journal Article |
| id | curtin-20.500.11937-46660 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:31:00Z |
| publishDate | 2014 |
| publisher | Wiley-Blackwell |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-466602017-09-13T14:08:47Z Cousins, siblings, or copies: the genomics of recurrent Staphylococcus aureus infections in chronic rhinosinusitis Drilling, A. Coombs, Geoffrey Tan, H. Pearson, J. Boase, S. Psaltis, A. Speck, P. Vreugde, S. Wormald, P. pulsed-field gel electrophoresis chronic rhinosinusitis persistent infection biofilms intracellular Staphylococcus aureus BackgroundStaphylococcus aureus infection is known to play a role in recalcitrant chronic rhinosinusitis (CRS). However, it is unknown if recurrent S. aureus infections are caused by the same strain or are due to independent acquisitions of different strains.MethodsSamples were collected from patients with CRS from July 2011 to August 2012. S. aureus was isolated from mucosal swabs and tissue specimens from patients who underwent surgery during the study period, or from swabs of areas of purulence taken in the postoperative period under endoscopic guidance. Pulsed-field gel electrophoresis was used to characterize S. aureus isolates.ResultsThirty-four patients were included in the study; 79% showed persistence of the same S. aureus strain in their paranasal sinuses (p = 0.001; H1 ? 50%). Furthermore, a significantly high frequency of patients with known biofilm status were positive for S. aureus biofilm (p = 0.002; H1 ? 50%). When patients were stratified according to disease evolution postsurgery, certain strains appeared to be more commonly associated with symptom persistence.ConclusionThe same S. aureus strain appears to persist in the paranasal sinuses of CRS patients despite multiple courses of culture-directed antibiotics. This suggests that conventional antimicrobial therapies in patients with CRS may not eliminate the organism. This may be partly explained by the formation of biofilms in the paranasal sinus region. 2014 Journal Article http://hdl.handle.net/20.500.11937/46660 10.1002/alr.21423 Wiley-Blackwell restricted |
| spellingShingle | pulsed-field gel electrophoresis chronic rhinosinusitis persistent infection biofilms intracellular Staphylococcus aureus Drilling, A. Coombs, Geoffrey Tan, H. Pearson, J. Boase, S. Psaltis, A. Speck, P. Vreugde, S. Wormald, P. Cousins, siblings, or copies: the genomics of recurrent Staphylococcus aureus infections in chronic rhinosinusitis |
| title | Cousins, siblings, or copies: the genomics of recurrent Staphylococcus aureus infections in chronic rhinosinusitis |
| title_full | Cousins, siblings, or copies: the genomics of recurrent Staphylococcus aureus infections in chronic rhinosinusitis |
| title_fullStr | Cousins, siblings, or copies: the genomics of recurrent Staphylococcus aureus infections in chronic rhinosinusitis |
| title_full_unstemmed | Cousins, siblings, or copies: the genomics of recurrent Staphylococcus aureus infections in chronic rhinosinusitis |
| title_short | Cousins, siblings, or copies: the genomics of recurrent Staphylococcus aureus infections in chronic rhinosinusitis |
| title_sort | cousins, siblings, or copies: the genomics of recurrent staphylococcus aureus infections in chronic rhinosinusitis |
| topic | pulsed-field gel electrophoresis chronic rhinosinusitis persistent infection biofilms intracellular Staphylococcus aureus |
| url | http://hdl.handle.net/20.500.11937/46660 |